Recombinant DNA Technology

<p>To create complementary sticky ends in the vector and DNA fragment (C)</p> Signup and view all the answers

<p>To introduce the recombinant DNA molecule into the host cell (C)</p> Signup and view all the answers

<p>The recombinant protein may not be produced. (D)</p> Signup and view all the answers

<p>To prevent the growth of non-transformed cells. (B)</p> Signup and view all the answers

<p>The use of an antibiotic for cell selection. (C)</p> Signup and view all the answers

<p>Grow in both ampicillin and tetracycline containing medium. (B)</p> Signup and view all the answers

<p>Screening (B)</p> Signup and view all the answers

<p>To increase the number of copies of the recombinant molecule (D)</p> Signup and view all the answers

<p>Transformed cells can grow in the presence of antibiotics while non-transformed cells cannot. (A)</p> Signup and view all the answers

<p>Screening (B)</p> Signup and view all the answers

<p>To eliminate colonies that do not contain the recombinant molecule (D)</p> Signup and view all the answers

<p>To produce the desired protein or other product (A)</p> Signup and view all the answers

<p>Replication (D)</p> Signup and view all the answers

<p>To confirm that the DNA insert is present in the recombinant molecule (B)</p> Signup and view all the answers

<p>Screening identifies colonies that express the recombinant DNA (D)</p> Signup and view all the answers

<p>SV40 (C)</p> Signup and view all the answers

<p>It can replicate in multiple host organisms (A)</p> Signup and view all the answers

<p>Baculovirus (A), SV40 (C), λgt11 (D)</p> Signup and view all the answers

<p>Baculovirus (B)</p> Signup and view all the answers

<p>λgt11 (D)</p> Signup and view all the answers

<p>To allow the vector to replicate independently of the host genome (B)</p> Signup and view all the answers

<p>To create a single, specific cut in the DNA sequence (C)</p> Signup and view all the answers

<p>To control the expression of cloned genes (C)</p> Signup and view all the answers

<p>It can produce high yields of proteins (A)</p> Signup and view all the answers

<p>The DNA sequence of the insert (D)</p> Signup and view all the answers

<p>To determine the linear order of nucleotide bases in DNA (C)</p> Signup and view all the answers

<p>Recombinant DNA technology provided the means to isolate and amplify specific DNA fragments, making sequencing more efficient and accurate. (A)</p> Signup and view all the answers

<p>All of the above (D)</p> Signup and view all the answers

<p>The host cell gene is activated and starts expressing its protein. (B)</p> Signup and view all the answers

<p>It disrupts the function of a gene involved in regulating gene expression. (C)</p> Signup and view all the answers

<p>Mid 1970s (C)</p> Signup and view all the answers

<p>The determination of the linear order of nucleotide bases in DNA (A)</p> Signup and view all the answers

<p>It encodes the genetic information for an organism (D)</p> Signup and view all the answers

<p>1978 (D)</p> Signup and view all the answers

<p>Artificially created DNA (D)</p> Signup and view all the answers

<p>All of the above. (D)</p> Signup and view all the answers

<p>A foreign DNA fragment and a plasmid vector (C)</p> Signup and view all the answers

<p>The foreign DNA fragment disrupts the expression of the gene. (C)</p> Signup and view all the answers

<p>Producing multiple identical copies of a specific DNA fragment. (A)</p> Signup and view all the answers

<p>They cleave DNA at specific sequences, creating fragments suitable for recombination. (C)</p> Signup and view all the answers

<p>To join two DNA fragments together, creating a phosphodiester bond. (D)</p> Signup and view all the answers

<p>A DNA molecule containing sequences from multiple distinct sources. (B)</p> Signup and view all the answers

<p>All of the above. (D)</p> Signup and view all the answers

<p>It recognizes a specific sequence of nucleotides and cuts the DNA at a specific site. (A)</p> Signup and view all the answers

<p>Blunt ends, which have no overhangs. (A)</p> Signup and view all the answers

<p>It joins two compatible ends of DNA fragments, creating a phosphodiester bond. (A)</p> Signup and view all the answers

<p>The use of DNA ligase to join two DNA fragments. (B)</p> Signup and view all the answers

<p>Phosphodiester bond (D)</p> Signup and view all the answers

<p>It stimulated discussion and development of guidelines for responsible use of recombinant DNA technology. (B)</p> Signup and view all the answers

A DNA molecule used to carry foreign DNA into a host cell.

A specific piece of DNA that is to be cloned.

The process of joining two DNA fragments together using ligase enzyme.

The introduction of recombinant DNA into a host organism.

Identifying host organisms that successfully incorporated the recombinant DNA.

DNA that has been artificially formed by combining constituents from different organisms.

A cell that supports the replication of a recombinant molecule.

A group of cells that have successfully taken up recombinant DNA.

The process of identifying cells that contain desired recombinant DNA.

A segment of DNA introduced into a host organism's genome.

The process by which the inserted DNA is transcribed and translated into proteins.

The examination of recombinant molecules to verify their properties and functionality.

The process by which recombinant DNA is copied within a host cell.

The process where foreign DNA is expressed in living cells to produce proteins.

Colonies of bacteria that have successfully incorporated recombinant DNA and can grow in selective media.

Growth medium containing antibiotics like ampicillin or tetracycline to identify transformed organisms.

Specialized plasmids used to promote high levels of protein production from foreign DNA.

The process of creating proteins from introduced recombinant DNA within host organisms.

A method of combining DNA from different sources to create new genetic sequences.

Researchers who first produced a recombinant DNA molecule, awarded the Nobel Prize in Chemistry in 1980.

The process of making identical copies of a specific DNA fragment.

A small circular DNA molecule within bacteria that can replicate independently.

An enzyme that cuts DNA at specific nucleotide sequences to facilitate cloning.

DNA fragments with no overhangs, cut straight across by restriction enzymes.

DNA fragments with short overhangs, allowing them to stick together.

Making multiple identical copies of a specific gene.

An enzyme that joins two pieces of DNA together by forming phosphodiester bonds.

Award given to Cohen and Boyer for their work on recombinant DNA.

A 1975 meeting that established guidelines for recombinant DNA research.

In 1978, the first human hormone (insulin) produced using recombinant DNA technology.

Genes often carried by plasmids that confer resistance to antibiotics.

DNA formed from genetic material of two different species.

The process of combining genetic material from multiple sources to create new DNA sequences.

A tool used to insert foreign DNA into a host organism for replication.

A shuttle vector that can replicate in E. coli and Saccharomyces cerevisiae.

Vectors derived from viruses designed to infect host cells for gene expression.

An expression vector derived from bacteriophage λ, used for gene cloning in E. coli.

A virus that infects mammalian cells, used as a vector for gene cloning.

A viral vector that infects insect cells for high protein expression.

The protocol involves digesting DNA, using vectors, and inserting foreign genes.

DNA that is introduced into a host organism for cloning or expression.

A region of DNA that initiates transcription of a gene.

Disruption of a gene's expression by inserting foreign DNA.

A gene that is not expressed or is inactive in a certain condition.

The process of determining the order of nucleotides in DNA.

The building blocks of DNA; adenine, thymine, cytosine, guanine.

The development of methods to sequence DNA began in the 1970s.