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Recombinant DNA Technology Fundamentals

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Questions and Answers

What is the fundamental goal of laboratory geneticists?

To clone and create chimeric genes

To produce artificial DNA

To isolate, characterize, and manipulate genes (correct)

To create recombinant organisms

What is a recombinant organism?

An organism produced via artificial means

An organism with a different combination of alleles from its parents (correct)

An organism formed by recombining genetic material

An organism with artificially created DNA

What is the definition of recombinant DNA?

Artificial DNA created by combining two or more sequences that would not normally occur together (correct)

DNA containing a different combination of alleles from its parents

DNA produced via artificial means

DNA formed by recombining genetic material

What does recombinant DNA technology involve?

Inserting DNA molecules from two different species into a host organism Signup and view all the answers

What is the procedure used in recombinant DNA technology mainly used for?

Finding and manipulating specific genes Signup and view all the answers

In the context of genetics, why can finding a specific gene within a DNA sample be compared to finding a needle in a haystack?

Because the process of gene isolation is extremely time-consuming and challenging Signup and view all the answers

Who was amongst the first to produce a recombinant DNA molecule?

All of the above Signup and view all the answers

Which researchers applied for and were granted the first patent on recombinant DNA technology?

Herbert Boyer and Stanley N. Cohen Signup and view all the answers

What did Paul Berg receive a Nobel Prize in Chemistry for?

His fundamental studies of the biochemistry of nucleic acids Signup and view all the answers

What is the process known as when artificially-created DNA is reproduced?

DNA cloning Signup and view all the answers

What was the first human hormone produced by recombinant DNA technology?

Insulin Signup and view all the answers

What mechanism can plasmids use to distinguish between host cells that carry the vectors and those that do not?

Carrying antibiotic resistance genes Signup and view all the answers

What is the size range of most bacteria's circular DNA chromosome?

$1,000$ to $8,000$ kilobase pairs Signup and view all the answers

What technique is used in the construction of recombinant DNA to insert a foreign DNA fragment into a plasmid vector?

$DNA ligase$ Signup and view all the answers

True or False: Recombinant DNA only becomes useful when artificially-created DNA is not reproduced.

False Signup and view all the answers

What conference discussed the development of experimental guidelines for recombinant DNA technology?

International Conference on Recombinant DNA Molecules Signup and view all the answers