Recombinant DNA & Biotechnology Applications

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Questions and Answers

Which technique separates DNA fragments based on their size using an electric current?

  • Gene Therapy
  • DNA Fingerprinting
  • Transformation
  • Gel Electrophoresis (correct)

What is the purpose of transformation in the context of recombinant DNA technology?

  • Amplifying DNA fragments.
  • Separating DNA fragments by size.
  • Cutting DNA at specific sequences.
  • Introducing recombinant DNA into a host cell. (correct)

In gene therapy, what is the primary goal?

  • Modifying crops to increase yield.
  • Producing large quantities of specific proteins.
  • Replacing faulty genes with healthy ones. (correct)
  • Introducing new traits into an organism's offspring.

Which of the following is NOT a common application of recombinant DNA technology in medicine?

<p>Development of antibiotics (A)</p> Signup and view all the answers

What is a key benefit commonly associated with genetically modified (GM) crops?

<p>Better yield and pest resistance (D)</p> Signup and view all the answers

How is DNA fingerprinting, an application of recombinant DNA technology, primarily used?

<p>For criminal investigations. (D)</p> Signup and view all the answers

In environmental science, what is a potential application of genetically engineered bacteria?

<p>Waste cleanup. (C)</p> Signup and view all the answers

What was the purpose of inserting the Green Fluorescent Protein (GFP) gene from jellyfish into mosquito larvae?

<p>To make the larvae glow under UV light for tracking. (A)</p> Signup and view all the answers

A scientist is using gel electrophoresis. What result would they expect to see?

<p>Smaller DNA fragments migrate further than larger fragments. (A)</p> Signup and view all the answers

If a researcher aims to introduce a specific gene into plant cells to enhance drought resistance, which technique should they employ?

<p>Transformation (D)</p> Signup and view all the answers

Flashcards

Gel Electrophoresis

Separates DNA fragments by size using an electric current.

Transformation

Introducing recombinant DNA into a host cell.

Gene Therapy

Replacing faulty genes with healthy ones to treat diseases.

Bioremediation

Using genetically engineered organisms for waste cleanup.

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Forensic Applications

DNA fingerprinting in legal contexts.

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Genetically Modified (GM) Crops

Crops modified for increased yield or pest resistance.

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Green Fluorescent Protein (GFP)

Gene from jellyfish inserted into mosquito larvae makes them glow under UV light, for tracking modifications.

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Study Notes

  • Gel Electrophoresis separates DNA fragments by size using an electric current.
  • Transformation introduces recombinant DNA into a host cell.
  • Gene Therapy replaces faulty genes with healthy ones to treat genetic diseases.

Applications of Recombinant DNA & Biotechnology

  • Medicine uses recombinant DNA technology for insulin and hormone production.
  • Vaccines and gene therapy are results of applications of recombinant DNA tech.
  • Agriculture uses genetically modified (GM) crops for improved yield and pest resistance.
  • Forensics applies DNA fingerprinting for criminal investigations.
  • Environmental Science uses genetically engineered bacteria for waste cleanup.

Real-World Example

  • Green Fluorescent Protein (GFP) from jellyfish is inserted into mosquito larvae.
  • Modified larvae glow under UV light.
  • This process allows researchers to track genetic modifications effectively.

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