17 Questions
What is the nomenclature for a sequence of three nucleotides?
Trinucleotide
Which type of nucleotides are present in oligodeoxyribonucleotides (ODNs)?
T A C G
What is the main difference between oligodeoxyribonucleotides and oligoribonucleotides?
Presence of thymine vs. uracil
Which technology is used at the RNA level?
Antisense technology
What is the main application of nucleic acid technology for studying diseases and genetic disorders?
Transgenesis
What are aptamers used to target at the protein level?
Enzymes
What is the function of ribozymes in nucleic acid technology?
Splicing
What is the length of oligonucleotides?
100 or fewer bases long
What does U replace in oligoribonucleotides?
T
What is the main purpose of using microarray in nucleic acid technology?
Diagnostics
What does the term 'oligos' refer to?
A polymer chain
What is the name given to a 21-base containing oligonucleotide?
21-mer
Why are oligodeoxyribonucleotides and oligoribonucleotides highly susceptible to rapid degradation by structurally nonspecific intracellular nucleases?
They possess a negative charge
How do natural oligonucleotides accumulate in cells?
Receptor-mediated endocytosis
What is the backbone of a peptide nucleic acid (PNA)?
Aminoethyl glycine units
What is the technology in which short oligodeoxyribonucleotides can bind sequence-specifically to complementary segments of duplex DNA?
Triplex Technology
At which level does antisense oligonucleotide interruption of the flow of genetic information occur?
mRNA level in the cytoplasm
Test your knowledge of recombinant DNA applications, including the structural and physico-chemical properties of oligonucleotides, as well as anti-sense technology, triplex technology, aptamers, and ribozymes. This quiz covers techniques used in Pharmacy and Medicine.
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