Recombinant DNA Applications Quiz

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Questions and Answers

What is the nomenclature for a sequence of three nucleotides?

  • Trinucleotide (correct)
  • Oligonucleotide
  • Dinucleotide
  • Mononucleotide

Which type of nucleotides are present in oligodeoxyribonucleotides (ODNs)?

  • T A U G
  • U A C G
  • T A C G (correct)
  • C G A T

What is the main difference between oligodeoxyribonucleotides and oligoribonucleotides?

  • Function in protein synthesis
  • Structure of the sugar molecule
  • Presence of thymine vs. uracil (correct)
  • Number of nucleotides

Which technology is used at the RNA level?

<p>Antisense technology (C)</p> Signup and view all the answers

What is the main application of nucleic acid technology for studying diseases and genetic disorders?

<p>Transgenesis (D)</p> Signup and view all the answers

What are aptamers used to target at the protein level?

<p>Enzymes (A)</p> Signup and view all the answers

What is the function of ribozymes in nucleic acid technology?

<p>Splicing (A)</p> Signup and view all the answers

What is the length of oligonucleotides?

<p>100 or fewer bases long (D)</p> Signup and view all the answers

What does U replace in oligoribonucleotides?

<p>T (B)</p> Signup and view all the answers

What is the main purpose of using microarray in nucleic acid technology?

<p>Diagnostics (A)</p> Signup and view all the answers

What does the term 'oligos' refer to?

<p>A polymer chain (D)</p> Signup and view all the answers

What is the name given to a 21-base containing oligonucleotide?

<p>21-mer (D)</p> Signup and view all the answers

Why are oligodeoxyribonucleotides and oligoribonucleotides highly susceptible to rapid degradation by structurally nonspecific intracellular nucleases?

<p>They possess a negative charge (B)</p> Signup and view all the answers

How do natural oligonucleotides accumulate in cells?

<p>Receptor-mediated endocytosis (B)</p> Signup and view all the answers

What is the backbone of a peptide nucleic acid (PNA)?

<p>Aminoethyl glycine units (D)</p> Signup and view all the answers

What is the technology in which short oligodeoxyribonucleotides can bind sequence-specifically to complementary segments of duplex DNA?

<p>Triplex Technology (C)</p> Signup and view all the answers

At which level does antisense oligonucleotide interruption of the flow of genetic information occur?

<p>mRNA level in the cytoplasm (A)</p> Signup and view all the answers

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