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Nucleic Acid Technology Quiz

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Questions and Answers

What are the structural and physico-chemical properties of oligonucleotides?

They are double-stranded RNA segments with no specific length.

They are long polymeric segments of DNA or RNA, generally more than 100 bases long.

They are single-stranded DNA segments with no specific length.

They are short polymeric segments of DNA or RNA, generally 100 or fewer bases long. (correct)

What is the nomenclature for a segment with 5 monomers?

Pentanucleotide (correct)

Quintanucleotide

Quinquenucleotide

Pentanucleoside

Which technology is used at the RNA level in nucleic acid technology?

Ribozyme technology

Anti-sense technology (correct)

Triplex technology

Aptamer technology

What is the substitution for thymine (T) in oligoribonucleotides?

Uracil (U) Signup and view all the answers

What is the name given to a 21-base containing oligonucleotide?

21-mer Signup and view all the answers

What is the function of peptide nucleic acid (PNA) in relation to oligonucleotides?

Serve as the backbone of the polymer with improved physiochemical characteristics Signup and view all the answers

What is the main feature of alkyl phosphonate in the context of oligonucleotides?

It enhances the stability of oligonucleotides Signup and view all the answers

What is the therapeutic application of Fomivirsen (Vitravene)?

Treatment of human cytomegalovirus (CMV) induced retinitis in AIDS patients Signup and view all the answers

What is the function of aptamers?

Bind to proteins or other small molecules Signup and view all the answers

What is the primary function of ribozymes?

Cleave covalent bonds, generally degrading a target RNA Signup and view all the answers

What is the mechanism of action of antisense technology at the mRNA level in the cytoplasm?

Masking the ribosome binding site Signup and view all the answers

What is the classification of Pegaptanib sodium injection (Macugen)?

Aptamer Signup and view all the answers

What is the role of natural ribozymes in the context of RNA translation?

Functional part of the ribosome, composed of RNA tertiary structural motifs Signup and view all the answers

What is the function of triplex technology in relation to DNA?

Short oligodeoxyribonucleotides bind sequence-specifically to complementary segments of duplex DNA Signup and view all the answers

What is the primary function of antisense DNA in the context of mRNA?

Complementary to mRNA and induce ribonuclease H (RNase H) action on RNA-RNA / DNA-RNA duplex Signup and view all the answers