Real-Time PCR Optimization
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Questions and Answers

What is one way to optimize the efficiency of qPCR?

  • Amplify long targets
  • Amplify targets with significant GC stretches
  • Amplify targets with high GC content
  • Amplify short targets (correct)

    • What is the ideal GC content for amplicons in qPCR optimization?

  • Close to 70%
  • Close to 50% (correct)
  • Close to 90%
  • Close to 30%

    • What should be considered when designing primers for qPCR?

  • Binding sites with high GC content
  • Unique binding sites for the genome (correct)
  • Binding sites within introns
  • Binding sites with high similarity to other genes

    • What can cause false positive results in qPCR?

    <p>Dimerization and self-folding of primers and probes</p> Signup and view all the answers

    How can primer-dimers be detected in qPCR optimization?

    <p>Gel electrophoresis</p> Signup and view all the answers

    What is the ideal amplicon size range for qPCR optimization?

    <p>Between 60 and 200bp</p> Signup and view all the answers

    What is the ideal GC content for amplicons in qPCR optimization?

    <p>Close to 50%</p> Signup and view all the answers

    What should be considered when designing primers for qPCR?

    <p>Unique binding sites for the genome</p> Signup and view all the answers

    What can cause false positive results in qPCR?

    <p>Dimerization and self-folding of primers and probes</p> Signup and view all the answers

    How can primer-dimers be detected in qPCR optimization?

    <p>Gel electrophoresis</p> Signup and view all the answers

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