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Questions and Answers
What is one way to optimize the efficiency of qPCR?
What is one way to optimize the efficiency of qPCR?
- Amplify long targets
- Amplify targets with significant GC stretches
- Amplify targets with high GC content
- Amplify short targets (correct)
What is the ideal GC content for amplicons in qPCR optimization?
What is the ideal GC content for amplicons in qPCR optimization?
- Close to 70%
- Close to 50% (correct)
- Close to 90%
- Close to 30%
What should be considered when designing primers for qPCR?
What should be considered when designing primers for qPCR?
- Binding sites with high GC content
- Unique binding sites for the genome (correct)
- Binding sites within introns
- Binding sites with high similarity to other genes
What can cause false positive results in qPCR?
What can cause false positive results in qPCR?
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How can primer-dimers be detected in qPCR optimization?
How can primer-dimers be detected in qPCR optimization?
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What is the ideal amplicon size range for qPCR optimization?
What is the ideal amplicon size range for qPCR optimization?
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What is the ideal GC content for amplicons in qPCR optimization?
What is the ideal GC content for amplicons in qPCR optimization?
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What should be considered when designing primers for qPCR?
What should be considered when designing primers for qPCR?
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What can cause false positive results in qPCR?
What can cause false positive results in qPCR?
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How can primer-dimers be detected in qPCR optimization?
How can primer-dimers be detected in qPCR optimization?
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