Podcast
Questions and Answers
What is the main principle behind the bilirubin test using reagent strips?
What is the main principle behind the bilirubin test using reagent strips?
- Reduction of bilirubin by ascorbic acid
- Hydrolysis of bilirubin glucuronide in urine
- Oxidation reaction of bilirubin in alkaline medium
- Coupling reaction of bilirubin with a diazonium salt in acid medium (correct)
What color change does Multistix exhibit when reacting with bilirubin?
What color change does Multistix exhibit when reacting with bilirubin?
- Yellow to orange
- Cream-buff to tan (correct)
- Violet to black
- Light pink to dark blue
How much bilirubin can Chemstrip detect in urine?
How much bilirubin can Chemstrip detect in urine?
- 0.8 mg per deciliter
- 0.2 mg per deciliter
- 0.5 mg per deciliter (correct)
- 1.0 mg per deciliter
What can cause false-negative results in bilirubin testing using reagent strips?
What can cause false-negative results in bilirubin testing using reagent strips?
Which substance does NOT interfere with bilirubin testing results?
Which substance does NOT interfere with bilirubin testing results?
What color change occurs with Chemstrip when bilirubin is present?
What color change occurs with Chemstrip when bilirubin is present?
Which of the following statements about the bilirubin test is NOT correct?
Which of the following statements about the bilirubin test is NOT correct?
Flashcards
Reagent Strip Bilirubin Test
Reagent Strip Bilirubin Test
A laboratory test used to detect bilirubin in urine.
Bilirubin
Bilirubin
A substance that is a breakdown product of heme, the iron-containing part of hemoglobin.
Diazonium Salt
Diazonium Salt
A chemical compound used in the reagent strip bilirubin test to react with bilirubin and produce a color change.
Coupling Reaction
Coupling Reaction
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Detection Limit
Detection Limit
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Ascorbic Acid
Ascorbic Acid
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Rifampin
Rifampin
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Study Notes
Reagent Strip Method for Bilirubin Detection
- Bilirubin detection relies on a coupling reaction with a diazonium salt in an acidic environment.
- Normal urine does not contain detectable bilirubin.
- Different reagent strip tests utilize varying diazonium salts, leading to different color changes and detection limits.
- Multistix uses diazotized 2,4-dichloroaniline, changing from cream-buff to tan within 20 seconds. This method detects 0.8 mg/dL of bilirubin in urine.
- Chemstrip employs 2,6-dichlorobenzene-diazonium tetrafluoroborate, exhibiting a pink to violet color change in 30–60 seconds. This test detects 0.5 mg/dL of bilirubin in urine.
- Urine samples need to be fresh; bilirubin glucuronide hydrolyzes rapidly to less reactive free bilirubin.
- Oxidation of bilirubin, particularly with exposure to light, can lead to false-negative results.
- High levels of ascorbic acid and nitrite can decrease bilirubin readings.
- Certain drug metabolites, like phenazopyridine (Pyridium), create a reddish color mimicking bilirubin, interfering with the test.
- Rifampin and high concentrations of chlorpromazine metabolites yield false-positive results, while salicylates do not affect the test.
- Urobilinogen does not influence the bilirubin test results.
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