Reagent Strip Method for Bilirubin Detection
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Questions and Answers

What is the main principle behind the bilirubin test using reagent strips?

  • Reduction of bilirubin by ascorbic acid
  • Hydrolysis of bilirubin glucuronide in urine
  • Oxidation reaction of bilirubin in alkaline medium
  • Coupling reaction of bilirubin with a diazonium salt in acid medium (correct)
  • What color change does Multistix exhibit when reacting with bilirubin?

  • Yellow to orange
  • Cream-buff to tan (correct)
  • Violet to black
  • Light pink to dark blue
  • How much bilirubin can Chemstrip detect in urine?

  • 0.8 mg per deciliter
  • 0.2 mg per deciliter
  • 0.5 mg per deciliter (correct)
  • 1.0 mg per deciliter
  • What can cause false-negative results in bilirubin testing using reagent strips?

    <p>Oxidation of bilirubin due to prolonged exposure to light</p> Signup and view all the answers

    Which substance does NOT interfere with bilirubin testing results?

    <p>Salicylates</p> Signup and view all the answers

    What color change occurs with Chemstrip when bilirubin is present?

    <p>Pink to violet at a temperature of 30–60 seconds</p> Signup and view all the answers

    Which of the following statements about the bilirubin test is NOT correct?

    <p>Bilirubin testing can be performed on aged urine samples.</p> Signup and view all the answers

    Study Notes

    Reagent Strip Method for Bilirubin Detection

    • Bilirubin detection relies on a coupling reaction with a diazonium salt in an acidic environment.
    • Normal urine does not contain detectable bilirubin.
    • Different reagent strip tests utilize varying diazonium salts, leading to different color changes and detection limits.
    • Multistix uses diazotized 2,4-dichloroaniline, changing from cream-buff to tan within 20 seconds. This method detects 0.8 mg/dL of bilirubin in urine.
    • Chemstrip employs 2,6-dichlorobenzene-diazonium tetrafluoroborate, exhibiting a pink to violet color change in 30–60 seconds. This test detects 0.5 mg/dL of bilirubin in urine.
    • Urine samples need to be fresh; bilirubin glucuronide hydrolyzes rapidly to less reactive free bilirubin.
    • Oxidation of bilirubin, particularly with exposure to light, can lead to false-negative results.
    • High levels of ascorbic acid and nitrite can decrease bilirubin readings.
    • Certain drug metabolites, like phenazopyridine (Pyridium), create a reddish color mimicking bilirubin, interfering with the test.
    • Rifampin and high concentrations of chlorpromazine metabolites yield false-positive results, while salicylates do not affect the test.
    • Urobilinogen does not influence the bilirubin test results.

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    Description

    This quiz explores the reagent strip method used for bilirubin detection in urine. It covers various diazonium salts employed in different tests and their corresponding colorimetric changes. Understand the implications of sample freshness and possible interferences in bilirubin readings.

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