Reading Volume on the He Micropipette: Accuracy and Precision

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Questions and Answers

What is the correct way to remove a tip from the sample liquid when operating a micropipette?

  • Withdraw the pipette with the plunger fully depressed (correct)
  • Allow residual liquid to remain on the outside of the tip
  • Remove the tip before withdrawing from the sample liquid
  • Depress the plunger to the second stop

What should you do after attaching the disposable tip to the micropipette?

  • Depress the plunger to the second stop
  • Start dispensing the sample (correct)
  • Touch the tip end to the side wall of the receiving vessel
  • Attach a colored tip for accurate measurements

What should be done to ensure there is no liquid remaining on the outside of the tip after withdrawing it from the sample liquid?

  • Withdraw the tip before removing it from the sample liquid
  • Depress the plunger to the first stop
  • Properly depress the plunger to expel any residual liquid (correct)
  • Remove the tip slowly to avoid liquid spillage

What should be done before depressing the plunger to dispense the sample when operating a micropipette?

<p>Touch the tip end to the side wall of the receiving vessel (A)</p> Signup and view all the answers

What is the purpose of allowing a few seconds pause after drawing up the sample into the plastic tip?

<p>To ensure that full volume of sample is drawn into the plastic tip (C)</p> Signup and view all the answers

What is the correct step for proper droplet removal when operating a micropipette?

<p>Depressing to expel residual liquid at second stop (D)</p> Signup and view all the answers

What action should be taken after dispense and before withdrawing the pipette from a receiving vessel when operating a micropipette?

<p>Depressing to expel any residual liquid in the tip (D)</p> Signup and view all the answers

When should you discard a tip used in sampling with a micropipette?

<p>After properly withdrawing from a receiving vessel (B)</p> Signup and view all the answers

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