Reading Volume on the He Micropipette: Accuracy and Precision

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What is the correct way to remove a tip from the sample liquid when operating a micropipette?

Withdraw the pipette with the plunger fully depressed

What should you do after attaching the disposable tip to the micropipette?

Start dispensing the sample

What should be done to ensure there is no liquid remaining on the outside of the tip after withdrawing it from the sample liquid?

Properly depress the plunger to expel any residual liquid

What should be done before depressing the plunger to dispense the sample when operating a micropipette?

Touch the tip end to the side wall of the receiving vessel

What is the purpose of allowing a few seconds pause after drawing up the sample into the plastic tip?

To ensure that full volume of sample is drawn into the plastic tip

What is the correct step for proper droplet removal when operating a micropipette?

Depressing to expel residual liquid at second stop

What action should be taken after dispense and before withdrawing the pipette from a receiving vessel when operating a micropipette?

Depressing to expel any residual liquid in the tip

When should you discard a tip used in sampling with a micropipette?

After properly withdrawing from a receiving vessel

Learn about reading the volume on a He micropipette, as well as understanding accuracy and precision in pipetting. Explore how accuracy is determined by calculating the average value of replicates and specified as Mean Absolute Percentage Error (M).

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