Podcast
Questions and Answers
Which of the following characteristics distinguishes rabies virus?
Which of the following characteristics distinguishes rabies virus?
- It is a naked DNA virus.
- It is a naked RNA virus.
- It is a non-enveloped DNA virus.
- It is an enveloped RNA virus. (correct)
Rabies virus is transmitted through direct contact with saliva from an infected animal.
Rabies virus is transmitted through direct contact with saliva from an infected animal.
True (A)
What is the scientific name of the family to which rabies virus belongs?
What is the scientific name of the family to which rabies virus belongs?
Rhabdoviridae
Rabies is a ______ disease, meaning it can be transmitted from animals to humans.
Rabies is a ______ disease, meaning it can be transmitted from animals to humans.
Match the following aspects of rabies virus with their corresponding descriptions:
Match the following aspects of rabies virus with their corresponding descriptions:
Which of the following is NOT a method commonly used for rabies virus diagnosis?
Which of the following is NOT a method commonly used for rabies virus diagnosis?
Rabies virus can be cultivated in cell cultures.
Rabies virus can be cultivated in cell cultures.
What does PEP stand for in the context of rabies prevention?
What does PEP stand for in the context of rabies prevention?
What is the primary method for preventing rabies transmission in both animals and humans?
What is the primary method for preventing rabies transmission in both animals and humans?
What are the primary hosts for rabies virus infections?
What are the primary hosts for rabies virus infections?
Once rabies symptoms develop, there is a highly effective cure available.
Once rabies symptoms develop, there is a highly effective cure available.
What are the two primary components of post-exposure prophylaxis (PEP) for rabies?
What are the two primary components of post-exposure prophylaxis (PEP) for rabies?
The rabies virus can survive for extended periods outside of a living host.
The rabies virus can survive for extended periods outside of a living host.
What is the key role of the rabies virus' glycoprotein?
What is the key role of the rabies virus' glycoprotein?
The rabies virus, a member of the ______ family, is known for its neurotropic nature.
The rabies virus, a member of the ______ family, is known for its neurotropic nature.
Match the following terms related to rabies with their descriptions:
Match the following terms related to rabies with their descriptions:
The [BLANK] protein of the rabies virus is crucial for the assembly and budding process of new viral particles.
The [BLANK] protein of the rabies virus is crucial for the assembly and budding process of new viral particles.
Match the following cell types with their roles in rabies virus infection.
Match the following cell types with their roles in rabies virus infection.
What is the primary method of preventing rabies in humans?
What is the primary method of preventing rabies in humans?
Which of the following environmental factors can inactivate the rabies virus?
Which of the following environmental factors can inactivate the rabies virus?
Once clinical symptoms of rabies appear, the disease is nearly always fatal.
Once clinical symptoms of rabies appear, the disease is nearly always fatal.
The rabies virus is highly stable outside the host and can withstand harsh environmental conditions.
The rabies virus is highly stable outside the host and can withstand harsh environmental conditions.
What is the most common mode of rabies transmission?
What is the most common mode of rabies transmission?
Describe the role of the World Health Organisation (WHO) in combating rabies.
Describe the role of the World Health Organisation (WHO) in combating rabies.
The rabies virus exhibits a unique pathogenicity known as ______, which means it targets the nervous system.
The rabies virus exhibits a unique pathogenicity known as ______, which means it targets the nervous system.
Match the following viral proteins with their primary function:
Match the following viral proteins with their primary function:
Which of the following is NOT a mode of rabies transmission?
Which of the following is NOT a mode of rabies transmission?
The glycoprotein (G) is a major target for the immune system and is a key component of rabies vaccines.
The glycoprotein (G) is a major target for the immune system and is a key component of rabies vaccines.
What is the role of the Large protein (L) in the rabies virus?
What is the role of the Large protein (L) in the rabies virus?
Rabies virus exhibits a strong affinity for neurons, infecting and destroying them.
Rabies virus exhibits a strong affinity for neurons, infecting and destroying them.
Which of the following is NOT a method for cultivating rabies virus?
Which of the following is NOT a method for cultivating rabies virus?
The time between infection and the onset of rabies symptoms is called the ______ period.
The time between infection and the onset of rabies symptoms is called the ______ period.
What is the primary receptor on host cells that rabies virus attaches to?
What is the primary receptor on host cells that rabies virus attaches to?
Match the following viral proteins involved in rabies replication with their corresponding functions:
Match the following viral proteins involved in rabies replication with their corresponding functions:
How does rabies virus enter the host cell?
How does rabies virus enter the host cell?
The rabies virus can evade the host immune response, which contributes to its ability to spread within the nervous system.
The rabies virus can evade the host immune response, which contributes to its ability to spread within the nervous system.
What are two key steps in the replication of rabies virus after it enters a host cell?
What are two key steps in the replication of rabies virus after it enters a host cell?
Which of the following viruses is NOT part of the Lyssavirus genus?
Which of the following viruses is NOT part of the Lyssavirus genus?
Lyssaviruses are known for their ability to infect a wide range of animals, but they cannot infect humans.
Lyssaviruses are known for their ability to infect a wide range of animals, but they cannot infect humans.
What is the primary characteristic that gives lyssaviruses their name and describes their main target in the body?
What is the primary characteristic that gives lyssaviruses their name and describes their main target in the body?
The rabies virus has a distinctive ______ shape.
The rabies virus has a distinctive ______ shape.
Match the following rabies virus proteins with their respective functions:
Match the following rabies virus proteins with their respective functions:
Which of the following is NOT a distinguishable characteristic of the rabies virus?
Which of the following is NOT a distinguishable characteristic of the rabies virus?
The rabies virus is surrounded by an envelope that is derived from the host cell membrane.
The rabies virus is surrounded by an envelope that is derived from the host cell membrane.
What is the approximate size of the rabies virus genome in nucleotides?
What is the approximate size of the rabies virus genome in nucleotides?
Flashcards
Rabies Virus Infection Process
Rabies Virus Infection Process
Attachment to host cell receptors initiates rabies infection.
Viral RNA-dependent RNA Polymerase
Viral RNA-dependent RNA Polymerase
Enzyme responsible for viral RNA transcription and replication.
Rabies Virus Stability
Rabies Virus Stability
Rabies virus is fragile outside the host, sensitive to environment.
Heat Sensitivity
Heat Sensitivity
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Drying Effect
Drying Effect
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Modes of Transmission
Modes of Transmission
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Neurotropism
Neurotropism
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Environmental Inactivation
Environmental Inactivation
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Widespread Vaccination
Widespread Vaccination
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Vaccination of Domestic Animals
Vaccination of Domestic Animals
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Post-Exposure Prophylaxis (PEP)
Post-Exposure Prophylaxis (PEP)
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Wound Care
Wound Care
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Rabies Immunoglobulin (RIG)
Rabies Immunoglobulin (RIG)
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Public Health Education
Public Health Education
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No Cure for Established Infection
No Cure for Established Infection
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Neurological Damage
Neurological Damage
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Rabies Virus
Rabies Virus
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First Rabies Vaccination
First Rabies Vaccination
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Rhabdoviruses
Rhabdoviruses
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Rabies Virus Glycoprotein
Rabies Virus Glycoprotein
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Rabies Diagnosis
Rabies Diagnosis
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Human Rabies Immunoglobulin (HRIG)
Human Rabies Immunoglobulin (HRIG)
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Immune Escape Strategy
Immune Escape Strategy
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Rabies Virus Life Cycle
Rabies Virus Life Cycle
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Rabies virus transmission
Rabies virus transmission
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Long incubation period
Long incubation period
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Progressive neurological disease
Progressive neurological disease
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Immune evasion
Immune evasion
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Vero cells
Vero cells
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Animal models for cultivation
Animal models for cultivation
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Viral replication steps
Viral replication steps
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mRNA translation
mRNA translation
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Zoonotic potential
Zoonotic potential
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Rabies lyssavirus
Rabies lyssavirus
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Bullet-shaped virion
Bullet-shaped virion
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Negative-sense RNA
Negative-sense RNA
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Glycoprotein spikes
Glycoprotein spikes
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Nucleoprotein (N)
Nucleoprotein (N)
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Matrix protein (M)
Matrix protein (M)
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Lyssavirus
Lyssavirus
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Vaccine-preventable
Vaccine-preventable
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Zoonotic disease
Zoonotic disease
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Central Nervous System (CNS)
Central Nervous System (CNS)
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Symptoms of rabies
Symptoms of rabies
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Diagnosis Techniques
Diagnosis Techniques
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Study Notes
Rabies Virus (Rhabdovirus)
- Rabies is a zoonotic, vaccine-preventable viral disease affecting the central nervous system.
- Transmission is commonly through animal bites (typically by dogs).
- The virus infects mammals, including dogs, cats, livestock, and wildlife.
- Rabies is nearly always fatal once clinical symptoms appear.
- Symptoms appear in varying timescales (from a few days to several years).
Classification
- Rabies virus belongs to the Mononegavirales order.
- Viruses within that order have a negative-sense, single-stranded RNA genome.
- Rabies virus is a Lyssavirus within the Rhabdoviridae family, distinguishable by its bullet-shaped virions.
Structure and Morphology
- Rabies virions have a bullet-like shape.
- They are enveloped viruses with glycoprotein spikes crucial for attachment to host cells.
- The virus contains a tightly coiled RNA genome within a nucleocapsid.
Characteristics
- Stability: Sensitive to heat and desiccation (drying).
- Transmission: Primarily through animal bites (saliva).
- Neurotropism: Highly infective to the nervous system.
- Incubation: The time varies significantly.
- High Mortality: Almost always fatal without intervention.
Cultivation
- Methods used for cultivation include cell culture (e.g., Vero cells) and animal models (e.g., mice).
- Cell culture is more commonly used in modern practice.
Replication
- Attachment and Entry: The virus attaches to host cell receptors and enters through endocytosis.
- Uncoating: The viral envelope fuses with the host cell, releasing nucleocapsids.
- Transcription and Translation: The virus's RNA polymerase creates mRNA from the negative-sense RNA genome to translate viral proteins.
- Replication and Assembly: Viral protein and RNA genomes assemble into new virus particles.
- Budding: The new particles bud from the host cell to complete the cycle.
Diagnosis
- Diagnosis involves detecting the virus or viral antigens in infected tissues using techniques such as:
- Direct Fluorescent Antibody (DFA)
- Direct Rapid Immunohistochemistry (dRIT)
- Reverse Transcription-Polymerase Chain Reaction (RT-PCR)
- Mouse Inoculation Test (MIT)
Prodomal Phase Symptoms
- Flu-like symptoms: fever, headache, malaise, muscle aches.
- Discomfort at the bite area: tingling, itching, burning.
Neurological Phase Symptoms
- Changes in behaviour: anxiety, irritability, restlessness.
- Agitation, confusion, hallucinations, aggression.
- Dysphagia (difficulty swallowing) and excessive salivation.
- Hydrophobia (fear of water): painful spasms of the throat when attempting to swallow.
- Aerophobia (fear of drafts or air movement).
- Seizures.
- Progressive muscle weakness and paralysis.
Prevention and Treatment
- Animal Vaccination: A critical element for prevention, notably mass dog vaccination programs in affected regions.
- Post-Exposure Prophylaxis (PEP): Crucial if exposure occurs. Includes wound cleansing, administration of rabies immunoglobulin (RIG) and multiple vaccinations.
- Early Diagnosis and Treatment: Essential for successful outcomes.
Other Important Information
- Rabies virus is a member of the Rhabdoviridae family.
- Effective vaccines reduce risk, proper vaccination is crucial.
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