Recent Lessons

Show all results for ""

R-M System and Gene Cloning Quiz

Choose a study mode

Play Quiz

Study Flashcards

Spaced Repetition

Chat to Lesson

Podcast

Play an AI-generated podcast conversation about this lesson

Download our mobile app to listen on the go

Get App

Questions and Answers

What are the two contrasting enzymatic activities involved in restriction modification (R-M) systems?

A restriction endonuclease (REase) and a methyltransferase (MTase)

What is the function of the restriction endonuclease (REase) in the R-M system?

It recognizes and cleaves foreign DNA sequences at specific sites

How does the methyltransferase (MTase) activity ensure discrimination between self and non-self DNA?

It transfers methyl groups to the same specific DNA sequence within the host’s genome

What types of organisms do restriction-modification (R-M) systems occur in?

They occur in a wide variety of unicellular organisms, including eubacteria and archaea Signup and view all the answers

What is the functional role of restriction endonucleases (REases) in the restriction-modification (R-M) system?

REases cleave endo-nucleolytically at phosphodiester bonds, generating 5’ or 3’ overhangs or blunt ends Signup and view all the answers

Match the following components of the restriction-modification (R-M) system with their functions:

Restriction endonuclease (REase) = Recognizes and cleaves foreign DNA sequences at specific sites Methyltransferase (MTase) = Ensures discrimination between self and non-self DNA by transferring methyl groups Python = General-purpose programming JavaScript = Client-side scripting for web applications Signup and view all the answers

Match the following attributes of restriction-modification (R-M) systems with their significance:

Ubiquitous and considered primitive immune systems in bacteria = Indication of their success as a defense mechanism against invading genomes Directly target invading DNA elements = Mechanism involving restriction-modification (R-M) systems SQL = Database queries CSS = Styling web pages Signup and view all the answers

Match the following statements about restriction endonucleases (REases) with their functional roles:

Cleave endo-nucleolytically at phosphodiester bonds, generating 5’ or 3’ overhangs or blunt ends = Functional role of REases in the restriction-modification (R-M) system Mechanisms involving restriction-modification (R-M) systems = Function of the restriction endonuclease (REase) in the R-M system Python = General-purpose programming JavaScript = Client-side scripting for web applications Signup and view all the answers

Match the following descriptions of microbial evolution and diversity with their attributes for success:

Ability of bacteria to recognize and distinguish incoming foreign DNA from self DNA = Attribute for success in microbial evolution and diversity Mechanism involving restriction-modification (R-M) systems = One of the attributes for success in microbial evolution and diversity SQL = Database queries CSS = Styling web pages Signup and view all the answers

Match the following attributes of restriction-modification (R-M) systems with their prevalence across the prokaryotic kingdom:

Occur in a wide variety of unicellular organisms, including eubacteria and archaea = Prevalence across the prokaryotic kingdom Mechanisms involving restriction-modification (R-M) systems = Prevalence across the prokaryotic kingdom Python = General-purpose programming JavaScript = Client-side scripting for web applications Signup and view all the answers

Flashcards are hidden until you start studying