R-M System and Gene Cloning Quiz

A restriction endonuclease (REase) and a methyltransferase (MTase)

It recognizes and cleaves foreign DNA sequences at specific sites

It transfers methyl groups to the same specific DNA sequence within the host’s genome

<p>They occur in a wide variety of unicellular organisms, including eubacteria and archaea</p> Signup and view all the answers

<p>REases cleave endo-nucleolytically at phosphodiester bonds, generating 5’ or 3’ overhangs or blunt ends</p> Signup and view all the answers

<p>Restriction endonuclease (REase) = Recognizes and cleaves foreign DNA sequences at specific sites Methyltransferase (MTase) = Ensures discrimination between self and non-self DNA by transferring methyl groups Python = General-purpose programming JavaScript = Client-side scripting for web applications</p> Signup and view all the answers

<p>Ubiquitous and considered primitive immune systems in bacteria = Indication of their success as a defense mechanism against invading genomes Directly target invading DNA elements = Mechanism involving restriction-modification (R-M) systems SQL = Database queries CSS = Styling web pages</p> Signup and view all the answers

<p>Cleave endo-nucleolytically at phosphodiester bonds, generating 5’ or 3’ overhangs or blunt ends = Functional role of REases in the restriction-modification (R-M) system Mechanisms involving restriction-modification (R-M) systems = Function of the restriction endonuclease (REase) in the R-M system Python = General-purpose programming JavaScript = Client-side scripting for web applications</p> Signup and view all the answers

<p>Ability of bacteria to recognize and distinguish incoming foreign DNA from self DNA = Attribute for success in microbial evolution and diversity Mechanism involving restriction-modification (R-M) systems = One of the attributes for success in microbial evolution and diversity SQL = Database queries CSS = Styling web pages</p> Signup and view all the answers

<p>Occur in a wide variety of unicellular organisms, including eubacteria and archaea = Prevalence across the prokaryotic kingdom Mechanisms involving restriction-modification (R-M) systems = Prevalence across the prokaryotic kingdom Python = General-purpose programming JavaScript = Client-side scripting for web applications</p> Signup and view all the answers