Qualitative Tests of Protein Functional Groups
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Questions and Answers

Inorganic salt, an organic solvent (e.g., alcohol or chloroform), or ______.

heat

Without alteration of the molecule's primary structure, i.e., without cleavage of any of the primary chemical bonds that link one amino acid to another. Method: 1- Take 1 ml of protein Albumin. 2- Place it in a boiling water bath for 5-10 minutes. 3- Remove aside to cool to room temperature. 4- Note the ______.

change

Do you think free amino acids will give a positive result with ______ test? Why?

biuret

What is the least number of amino acids bonded together by peptide bonds that will respond positively to ______ test?

<p>biuret</p> Signup and view all the answers

After heating albumin at high temperature, does it still biologically active? Why?

<p>Yes</p> Signup and view all the answers

Can we use ______ method in fractionating mixture of proteins?

<p>salting out</p> Signup and view all the answers

Explain your result with ______.

<p>example</p> Signup and view all the answers

Take 1 ml of protein Albumin. Place it in a boiling water bath for 5-10 minutes. Remove aside to cool to room temperature. Note the ______.

<p>change</p> Signup and view all the answers

Do you think free amino acids will give a positive result with biuret test? ______?

<p>why</p> Signup and view all the answers

What is the least number of amino acids bonded together by peptide bonds that will respond positively to biuret ______?

<p>test</p> Signup and view all the answers

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