Protein Synthesis Overview
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Questions and Answers

What is the primary function of the genetic code?

  • To create the sequence of nucleotides in DNA.
  • To translate the sequence of nucleotides in DNA into a sequence of amino acids in a protein. (correct)
  • To determine the structure of a protein.
  • To regulate the rate of protein synthesis.
  • Which of the following statements is TRUE regarding the genetic code?

  • The genetic code is universal, meaning it is the same for all life forms but with a few exceptions. (correct)
  • The genetic code can be read in different reading frames.
  • The genetic code is identical for all organisms.
  • The genetic code is only applicable to eukaryotic organisms.
  • How many different codons are possible within a single genetic code?

  • 64 (correct)
  • 128
  • 4
  • 20
  • What is the specific role of a stop codon in protein synthesis?

    <p>To signal the end of a protein chain and terminate translation. (A)</p> Signup and view all the answers

    What is the function of the AUG codon in protein synthesis?

    <p>It marks the start of the translation process, and it codes for the amino acid methionine. (D)</p> Signup and view all the answers

    What does the degeneracy (redundancy) of the genetic code mean?

    <p>Multiple codons can code for the same amino acid. (C)</p> Signup and view all the answers

    What is the characteristic of the genetic code that ensures it is read as a continuous sequence of bases without gaps?

    <p>Commaless (A)</p> Signup and view all the answers

    Which of these is NOT a characteristic of the genetic code?

    <p>Ambiguous (C)</p> Signup and view all the answers

    What happens when one or two nucleotides are deleted or added in a sequence?

    <p>The reading frame is altered, changing the amino acid sequence from that point. (D)</p> Signup and view all the answers

    Which component is NOT required for the translational system?

    <p>DNA polymerase (A)</p> Signup and view all the answers

    What type of RNA carries genetic information from DNA to the ribosome?

    <p>Messenger RNA (mRNA) (C)</p> Signup and view all the answers

    What is the overall function of ribosomal RNA (rRNA) in protein synthesis?

    <p>To provide structural support and attachment sites in ribosomes. (A)</p> Signup and view all the answers

    What is the sedimentation unit size of eukaryotic ribosomes?

    <p>80S (A)</p> Signup and view all the answers

    Which of the following does NOT contribute to the structure of ribosomes?

    <p>Protein factors (B)</p> Signup and view all the answers

    What results from the addition of 'U' in the given sequence?

    <p>It leads to the production of Ser Pro Met Ala. (A)</p> Signup and view all the answers

    Which types of RNA are involved in the process of protein biosynthesis?

    <p>All three types: mRNA, rRNA, and tRNA (D)</p> Signup and view all the answers

    What is the primary difference in the location of transcription between prokaryotic and eukaryotic cells?

    <p>Transcription occurs in the nucleus in eukaryotes. (B)</p> Signup and view all the answers

    Which of the following processes occurs in eukaryotic cells but not in prokaryotic cells?

    <p>RNA processing (B)</p> Signup and view all the answers

    What type of RNA is produced after transcription in eukaryotic cells?

    <p>pre-mRNA (C)</p> Signup and view all the answers

    Which statement accurately describes the RNA processing in eukaryotic cells?

    <p>Mature mRNA exits the nucleus for translation. (C)</p> Signup and view all the answers

    In prokaryotic cells, how does translation relate to transcription?

    <p>Translation occurs in the ribosome immediately after transcription. (B)</p> Signup and view all the answers

    What feature of prokaryotic DNA distinguishes it from eukaryotic DNA?

    <p>Prokaryotic DNA exists in a circular form and is naked. (D)</p> Signup and view all the answers

    What happens to pre-mRNA before it can be used for translation in eukaryotic cells?

    <p>It is modified through RNA processing. (A)</p> Signup and view all the answers

    Which of the following best describes the transcription and translation process in prokaryotic cells?

    <p>Transcription and translation occur simultaneously due to the lack of a nuclear envelope. (A)</p> Signup and view all the answers

    Flashcards

    Translation

    The process where RNA is decoded to produce a specific amino acid sequence.

    Codon

    A sequence of three nucleotide bases in mRNA that codes for an amino acid.

    Genetic Code

    A set of rules that defines how nucleotide sequences correspond to amino acids.

    Start Codon

    The codon that signals the start of translation, usually AUG.

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    Termination Codon

    Codons that signal the end of protein synthesis (UAG, UGA, UAA).

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    Specificity of Genetic Code

    Each codon corresponds to a single specific amino acid.

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    Degenerate Genetic Code

    A single amino acid can be coded by multiple codons.

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    Non-overlapping Code

    The genetic code is read in separate codons, not overlapping.

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    Transcription

    The process of copying DNA to produce mRNA.

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    Prokaryotic Cells

    Cells without a nucleus where transcription and translation occur simultaneously.

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    Eukaryotic Cells

    Cells with a nucleus where transcription occurs in the nucleus and translation happens in the cytoplasm.

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    Pre-mRNA

    The initial RNA transcript that undergoes processing before becoming mature mRNA.

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    mRNA Processing

    The modifications made to pre-mRNA, including splicing, capping, and polyadenylation.

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    Mature mRNA

    The processed form of mRNA that exits the nucleus for translation.

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    Nucleosome/Chromatin

    DNA packaged with proteins in eukaryotic cells that regulates gene expression.

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    Reading Frame

    The way nucleotides are grouped into codons during translation.

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    Frameshift Mutation

    A mutation that alters the reading frame of mRNA by adding or deleting nucleotides.

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    mRNA

    Messenger RNA that carries genetic information from DNA to ribosomes.

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    rRNA

    Ribosomal RNA that forms the core of ribosome's structure and function.

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    tRNA

    Transfer RNA that brings amino acids to ribosomes during protein synthesis.

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    Aminoacyl tRNA Synthetases

    Enzymes that attach the correct amino acid to its corresponding tRNA.

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    Ribosome Subunits

    Ribosomes consist of two subunits that differ in size between prokaryotes and eukaryotes.

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    Protein Factors

    Proteins that assist in the process of translation.

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    Study Notes

    Protein Synthesis Overview

    • Protein synthesis involves transcription and translation.
    • Prokaryotes have no nucleus, so transcription and translation occur simultaneously in the cytoplasm.
    • Eukaryotes have a nucleus, separating transcription (in the nucleus) and translation (in the cytoplasm).

    Prokaryotic Protein Synthesis

    • Prokaryotic DNA is naked in the cytoplasm.
    • Transcription and translation occur simultaneously.

    Eukaryotic Protein Synthesis

    • Transcription occurs in the nucleus.
    • Pre-mRNA is processed before leaving the nucleus as mRNA.
    • mRNA then travels to the cytoplasm for translation.

    Translation

    • Translation requires a genetic code.
    • The genetic code is a dictionary.
    • Codons (three nucleotide bases) on mRNA correspond to specific amino acids.
    • The sequence of codons determines the amino acid sequence of the protein.

    Genetic Code Characteristics

    • Specific: Each codon codes for only one amino acid.
    • Universal: The genetic code is generally the same in all organisms.
    • Degenerate (Redundant): More than one codon can code for the same amino acid.
    • Non-overlapping & Commaless: Codons are read consecutively without punctuation.
    • Start codon (AUG): Initiates protein synthesis.
    • Stop codons (UAA, UAG, UGA): Signal the end of protein synthesis.

    Components of Translation

    • Amino acids: Building blocks of proteins.
    • tRNA (transfer RNA): Carries amino acids to the ribosome.
    • mRNA (messenger RNA): Carries the genetic code from DNA.
    • rRNA (ribosomal RNA): Forms ribosomes, the site of protein synthesis.
    • Aminoacyl-tRNA synthetases: Enzymes that attach amino acids to their corresponding tRNAs.
    • Ribosomes: Sites of protein synthesis.
    • Protein factors: Help with initiation, elongation, and termination of translation.
    • ATP & GTP: Provide energy for translation.

    Types of RNA

    • mRNA (messenger RNA): Carries the genetic code from DNA.
    • tRNA (transfer RNA): Carries amino acids to the ribosome.
    • rRNA (ribosomal RNA): Forms ribosomes.

    Ribosomes

    • Ribosomes are the sites of protein synthesis.
    • Ribosomes have large and small subunits (e.g., prokaryotic 70S, eukaryotic 80S).
    • They provide attachment sites for mRNA and tRNA, facilitating protein synthesis.

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    Description

    Explore the fundamentals of protein synthesis, focusing on the key differences between prokaryotic and eukaryotic processes. Understand the roles of transcription and translation, and learn how genetic codes dictate protein formation. This quiz will test your knowledge of these crucial biological concepts.

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