Protein Synthesis Initiation in Prokaryotes and Eukaryotes
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Questions and Answers

Which of the following is NOT a requirement for RNA polymerase during transcription?

  • A DNA template
  • A divalent metal ion (Mg2+ or Mn2+)
  • Deoxyribonucleoside triphosphates (correct)
  • Ribonucleoside triphosphates
  • What is the role of RNA polymerase in the process of transcription?

  • Initiating and elongating RNA chains (correct)
  • Assisting in protein synthesis at the ribosome
  • Providing a primer for RNA synthesis
  • Reading mRNA start and stop signals
  • In which direction does the synthesis occur during RNA and DNA synthesis?

  • Bidirectional
  • 3′-to-5′ direction
  • Random direction
  • 5′-to-3′ direction (correct)
  • What drives the synthesis of RNA during transcription?

    <p>Pyrophosphate hydrolysis</p> Signup and view all the answers

    Which nucleophilic attack occurs during the elongation of RNA chains?

    <p>3′–OH attacks the 5′ carbon of ribose sugar</p> Signup and view all the answers

    What type of information do RNA polymerases take from DNA templates?

    <p>Base composition</p> Signup and view all the answers

    What is the significance of the 'cap' structure added to the 5′ end of eukaryotic mRNA?

    <p>Facilitates translation initiation</p> Signup and view all the answers

    Why are tRNAs called adaptor molecules in protein synthesis?

    <p>They bring amino acids to the mRNA</p> Signup and view all the answers

    Which part of the tRNA molecule attaches to the amino acid?

    <p>CCA terminus</p> Signup and view all the answers

    How many nucleotides encode a single amino acid according to the genetic code?

    <p>Three nucleotides</p> Signup and view all the answers

    What term describes the phenomenon where most amino acids are encoded by more than one codon?

    <p>Redundancy</p> Signup and view all the answers

    What is the role of the poly(A) tail added to the 3′ end of eukaryotic mRNA?

    <p>Stabilizes the mRNA</p> Signup and view all the answers

    What type of amino acid is carried by the initiator tRNA in prokaryotes during protein synthesis?

    <p>Formylmethionine</p> Signup and view all the answers

    Which sequence precedes the initiating AUG codon in prokaryotes and base pairs with ribosomal RNA?

    <p>Shine–Dalgarno sequence</p> Signup and view all the answers

    What establishes the reading frame in protein synthesis by determining the order of three nonoverlapping nucleotides?

    <p>Location of the initiator codon</p> Signup and view all the answers

    Why is the genetic code nearly universal among most organisms?

    <p>To minimize deleterious effects of mutations</p> Signup and view all the answers

    In which organisms might we observe variations in the genetic code, such as codons typically representing stop codons encoding amino acids?

    <p>Mitochondria</p> Signup and view all the answers

    What are the distinguishing features of eukaryotic genes that contribute to their mosaicism?

    <p>Introns and exons alternate along the gene sequence</p> Signup and view all the answers

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