Protein Synthesis: DNA to Protein

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Questions and Answers

Which of the following is the primary function of proteins within a cell?

  • Synthesizing lipids
  • Catalyzing reactions, providing structure, and transporting molecules (correct)
  • Generating cellular energy
  • Storing genetic information

Transcription is the process where ribosomes build a protein based on the mRNA code.

False (B)

In eukaryotic cells, where does the process of transcription primarily occur?

nucleus

During translation, __________ molecules are responsible for carrying amino acids to the ribosome.

<p>tRNA</p> Signup and view all the answers

Match the following molecules with their roles in protein synthesis:

<p>mRNA = Carries genetic code from DNA to ribosome tRNA = Transports amino acids to the ribosome rRNA = Forms part of the ribosome DNA = Contains the original genetic code</p> Signup and view all the answers

Which enzyme is responsible for synthesizing mRNA during transcription?

<p>RNA polymerase (A)</p> Signup and view all the answers

Codons are sequences of three nucleotides on tRNA that pair with mRNA.

<p>False (B)</p> Signup and view all the answers

What type of bond is formed between amino acids during translation?

<p>peptide bond</p> Signup and view all the answers

The start codon, typically __________, codes for the amino acid methionine.

<p>AUG</p> Signup and view all the answers

Match each process to its location in a eukaryotic cell:

<p>Transcription = Nucleus Translation = Cytoplasm</p> Signup and view all the answers

What is the role of a stop codon in translation?

<p>To terminate protein synthesis (B)</p> Signup and view all the answers

Ribosomes are composed solely of protein.

<p>False (B)</p> Signup and view all the answers

What is the name given to the sequence of three nucleotides on tRNA that recognizes mRNA codons?

<p>anticodon</p> Signup and view all the answers

__________ is the process by which mRNA is created from a DNA template.

<p>transcription</p> Signup and view all the answers

Match the following molecules to their composition:

<p>DNA = Nucleotides containing deoxyribose sugar RNA = Nucleotides containing ribose sugar Protein = Amino acids</p> Signup and view all the answers

After translation, what may occur to a protein before it becomes fully functional?

<p>Folding and modification (B)</p> Signup and view all the answers

Each codon on mRNA codes for only one specific amino acid.

<p>True (A)</p> Signup and view all the answers

What is the final product of the translation process?

<p>polypeptide chain</p> Signup and view all the answers

The process of protein synthesis is crucial for creating proteins that serve various roles, including __________, structural support, and enzymatic activity.

<p>transport</p> Signup and view all the answers

Match the following processes with their description:

<p>Transcription = DNA to mRNA Translation = mRNA to protein</p> Signup and view all the answers

Flashcards

Protein Synthesis

The process of creating proteins from the genetic code in DNA.

DNA's Role

Molecule containing the genetic code for proteins, residing in the nucleus.

RNA's Role

Involved in transferring information from DNA to ribosomes.

Transcription

The first step of protein synthesis, where DNA is copied into mRNA.

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RNA Polymerase

Enzyme that creates mRNA by using DNA as a template.

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mRNA

RNA that carries the genetic code from the nucleus to the ribosome.

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Ribosome

Cellular structure where protein synthesis occurs.

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rRNA

A type of RNA that forms part of the ribosome structure.

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Translation

The second step where a protein is assembled by the ribosome based on the mRNA code.

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tRNA

RNA molecule that carries amino acids to the ribosome.

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Amino Acids

The building blocks (monomers) of proteins.

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Codon

A sequence of three mRNA nucleotides that codes for a specific amino acid.

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Anticodon

A sequence of three tRNA nucleotides that complements an mRNA codon.

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Peptide Bond

A bond that links amino acids together in a protein.

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Stop Codon

Signals the end of protein synthesis.

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Study Notes

Protein Synthesis Overview

  • Protein synthesis creates proteins based on DNA's genetic information.
  • Proteins serve essential functions like transport, structure, enzyme activity, and protection.
  • Protein synthesis takes place in cells.

Role of DNA and RNA

  • DNA houses the genetic code for proteins and resides in the nucleus.
  • RNA is essential for transferring DNA information to protein-making machinery.
  • RNA, a nucleic acid, shares similarities with DNA but has key differences.
  • Protein synthesis occurs in two main steps: transcription and translation.

Transcription

  • Transcription, the initial step, involves transcribing DNA into mRNA.
  • This occurs within the nucleus.
  • RNA polymerase, an enzyme, links complementary RNA bases to DNA, forming single-stranded mRNA.
  • mRNA undergoes editing before leaving the nucleus.
  • mRNA carries a message of RNA based on the DNA sequence.

mRNA and Ribosomes

  • In eukaryotes, mRNA can exit the nucleus and enter the cytoplasm.
  • Inside the cytoplasm, mRNA binds to a ribosome.
  • Ribosomes are responsible for protein synthesis.
  • Ribosomes are composed of ribosomal RNA (rRNA).

Translation

  • Translation, the second step, involves ribosomes building proteins based on the mRNA code.
  • Transfer RNA (tRNA) in the cytoplasm carries amino acids.
  • Amino acids are the building blocks (monomers) of proteins.
  • tRNA transports amino acids to the ribosome for protein construction.
  • The mRNA sequence determines which tRNAs enter, dictating the amino acid sequence.

Codons, Anticodons, and Amino Acids

  • Transfer RNA (tRNA) reads mRNA bases in triplets called codons.
  • Each tRNA has a complementary anticodon that pairs with the mRNA codon.
  • Each tRNA carries a specific amino acid determined by its anticodon.
  • For instance, the AUG codon on mRNA pairs with the UAC anticodon on tRNA, carrying methionine.
  • Methionine often serves as the starting amino acid in a protein (start codon).
  • Codon charts aid in determining which amino acid corresponds to each mRNA codon.
  • Multiple codons can code for the same amino acid.

The Process of Translation

  • The tRNA with the correct anticodon pairs with the mRNA codon and transfers its amino acid.
  • Amino acids are connected via peptide bonds, forming a growing polypeptide chain.
  • A stop codon at the end of the mRNA signals the termination of protein synthesis.
  • Stop codons do not code for an amino acid.
  • Translation produces a chain of amino acids in a specific sequence dictated by the mRNA.

Post-Translational Processing

  • DNA directs protein synthesis with assistance from mRNA, rRNA, and tRNA.
  • Protein folding and modification may occur post-translation.
  • The protein may require transport to its final destination.

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