Protein Stabilization and Substances Quiz
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Questions and Answers

What role do substances like sugar and glycine play in stabilizing proteins such as human growth hormone?

  • Acting as inhibitors to prevent protein denaturation
  • Acting as catalysts to speed up protein folding
  • Acting as preservatives to prevent protein degradation
  • Acting as bulking agents and stabilizers due to their interaction with water (correct)
  • Why is it recommended to refrigerate reconstituted human growth hormone (hGH) rather than freeze it?

  • Refrigeration slows down the degradation of hGH
  • Freezing can lead to the formation of crystals, which destabilize hGH
  • Refrigeration allows for better interaction between hGH and stabilizing substances
  • Freezing can lead to the formation of ice, which highly concentrates the remaining liquid (correct)
  • What is the purpose of placing amorphous substances like sugars in a glassy state?

  • To enhance the reactivity of the substances with water molecules
  • To act as a rigid glass, trapping molecules including proteins and preventing their movement (correct)
  • To facilitate the crystallization of the substances for better stability
  • To increase the solubility of the substances in liquid protein formulations
  • Why is glycine identified as a stabilizer in the presence of mannitol?

    <p>It allows for interactions, such as hydrogen bonding, between glycine and mannitol, contributing to the stabilization of proteins</p> Signup and view all the answers

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