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Questions and Answers
What role do substances like sugar and glycine play in stabilizing proteins such as human growth hormone?
What role do substances like sugar and glycine play in stabilizing proteins such as human growth hormone?
- Acting as inhibitors to prevent protein denaturation
- Acting as catalysts to speed up protein folding
- Acting as preservatives to prevent protein degradation
- Acting as bulking agents and stabilizers due to their interaction with water (correct)
Why is it recommended to refrigerate reconstituted human growth hormone (hGH) rather than freeze it?
Why is it recommended to refrigerate reconstituted human growth hormone (hGH) rather than freeze it?
- Refrigeration slows down the degradation of hGH
- Freezing can lead to the formation of crystals, which destabilize hGH
- Refrigeration allows for better interaction between hGH and stabilizing substances
- Freezing can lead to the formation of ice, which highly concentrates the remaining liquid (correct)
What is the purpose of placing amorphous substances like sugars in a glassy state?
What is the purpose of placing amorphous substances like sugars in a glassy state?
- To enhance the reactivity of the substances with water molecules
- To act as a rigid glass, trapping molecules including proteins and preventing their movement (correct)
- To facilitate the crystallization of the substances for better stability
- To increase the solubility of the substances in liquid protein formulations
Why is glycine identified as a stabilizer in the presence of mannitol?
Why is glycine identified as a stabilizer in the presence of mannitol?
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