Protein Photolysis and Spectroscopic Studies Quiz
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Questions and Answers

What is the main focus of the transient spectroscopic studies mentioned in the text?

  • Identifying structural changes in the photolysed state of Mb*CO
  • Exploring the enthalpic barrier to ligand rebinding from the pockee
  • Comparing the frequency and intensity of the Raman FeN'2 stretch mode
  • Investigating the dynamics of the protein during relaxation (correct)
  • What are the functionally important structural changes observed in the relaxation from Mb*CO to unligated myoglobin?

  • Compression and motion of the F helix towards its junction with the E helix
  • Increase in the enthalpic barrier decreasing the rate coefficient and lowering ligand-binding affinity
  • Increase in iron out-of-plane distance, decrease in Fe-proximal histidine bond length, and tilting of the proximal histidine (correct)
  • Decrease in the enthalpic part of the barrier to ligand rebinding from the pockee
  • What does the relaxation of the proximal haem environment from Mb*CO to unligated myoglobin conceptually resemble?

  • Decrease in Fe-proximal histidine bond length
  • Increase in the enthalpic barrier decreasing the rate coefficient
  • Allosteric R--> T transition of haemoglobin (correct)
  • Dramatic increase in the enthalpic part of the barrier to ligand rebinding
  • What does an increase in the enthalpic barrier to ligand rebinding from the pockee lead to?

    <p>Decrease in the associated rate coefficient and lowering of ligand-binding affinity</p> Signup and view all the answers

    What provides a basis for structural interpretation of the complex changes seen in an important spectroscopic marker?

    <p>The results from transient spectroscopic studies</p> Signup and view all the answers

    What determines the frequency and intensity of the Raman FeN'2 stretch mode?

    <p>The structural parameters</p> Signup and view all the answers

    What method was used to obtain snapshots of myoglobin before, during, and after its reaction with carbon monoxide?

    <p>X-ray crystallography at liquid-helium temperatures</p> Signup and view all the answers

    What information can help to decide the most fruitful approaches to questions about dynamics?

    <p>Results from spectroscopy and computation</p> Signup and view all the answers

    What type of models can be made using detailed structural information about the unstable Mb*CO complex?

    <p>Structural-kinetic models of the ligand binding process</p> Signup and view all the answers

    What is the goal of combining detailed structural information with the results of other approaches?

    <p>To create a comprehensive picture of how a simple protein works</p> Signup and view all the answers

    Which article has a misleading error introduced in the opening sentence of the first paragraph?

    <p>Multiple processing streams in occipitotemporal visual cortex</p> Signup and view all the answers

    What should have been colored differently in the Progress article about the ancient regulatory-protein family of WD-repeat proteins?

    <p>'WD-repeating units for the TUPI protein'</p> Signup and view all the answers

    When was the article 'The ancient regulatory-protein family of WD-repeat proteins' accepted?

    <p>23 September 1994</p> Signup and view all the answers

    What is the title of reference 1 in the provided text?

    <p>'Hemoglobin and Myoglobin in Their Reactions with Ligands'</p> Signup and view all the answers

    'Biochemistry' is referenced in the text for which purpose?

    <p>'Comparison with spectroscopy and computation'</p> Signup and view all the answers

    Which reference provides information about TUPI correction?

    <p>'Rothberg, L. J., Roberson, M. &amp; Jedju, T. M.'</p> Signup and view all the answers

    What is the main focus of the transient spectroscopic studies mentioned in the text?

    <p>Identifying structural changes in myoglobin</p> Signup and view all the answers

    What does an increase in the enthalpic barrier to ligand rebinding from the pockee lead to?

    <p>Decrease in the rate coefficient</p> Signup and view all the answers

    What provides a basis for structural interpretation of the complex changes seen in an important spectroscopic marker?

    <p>Relaxation of Mb*CO towards unligated structure</p> Signup and view all the answers

    What method was used to obtain snapshots of myoglobin before, during, and after its reaction with carbon monoxide?

    <p>Transient spectroscopy</p> Signup and view all the answers

    Which reference provides information about TUPI correction?

    <p>Reference 2</p> Signup and view all the answers

    'Biochemistry' is referenced in the text for which purpose?

    <p>To compare structural parameters</p> Signup and view all the answers

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