Protein Photolysis and Spectroscopic Studies Quiz

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What is the main focus of the transient spectroscopic studies mentioned in the text?

Identifying structural changes in the photolysed state of Mb*CO

Exploring the enthalpic barrier to ligand rebinding from the pockee

Comparing the frequency and intensity of the Raman FeN'2 stretch mode

Investigating the dynamics of the protein during relaxation (correct)

What are the functionally important structural changes observed in the relaxation from Mb*CO to unligated myoglobin?

Compression and motion of the F helix towards its junction with the E helix

Increase in the enthalpic barrier decreasing the rate coefficient and lowering ligand-binding affinity

Increase in iron out-of-plane distance, decrease in Fe-proximal histidine bond length, and tilting of the proximal histidine (correct)

Decrease in the enthalpic part of the barrier to ligand rebinding from the pockee

What does the relaxation of the proximal haem environment from Mb*CO to unligated myoglobin conceptually resemble?

Decrease in Fe-proximal histidine bond length

Increase in the enthalpic barrier decreasing the rate coefficient

Allosteric R--> T transition of haemoglobin (correct)

Dramatic increase in the enthalpic part of the barrier to ligand rebinding

What does an increase in the enthalpic barrier to ligand rebinding from the pockee lead to?

Decrease in the associated rate coefficient and lowering of ligand-binding affinity Signup and view all the answers

What provides a basis for structural interpretation of the complex changes seen in an important spectroscopic marker?

The results from transient spectroscopic studies Signup and view all the answers

What determines the frequency and intensity of the Raman FeN'2 stretch mode?

The structural parameters Signup and view all the answers

What method was used to obtain snapshots of myoglobin before, during, and after its reaction with carbon monoxide?

X-ray crystallography at liquid-helium temperatures Signup and view all the answers

What information can help to decide the most fruitful approaches to questions about dynamics?

Results from spectroscopy and computation Signup and view all the answers

What type of models can be made using detailed structural information about the unstable Mb*CO complex?

Structural-kinetic models of the ligand binding process Signup and view all the answers

What is the goal of combining detailed structural information with the results of other approaches?

To create a comprehensive picture of how a simple protein works Signup and view all the answers

Which article has a misleading error introduced in the opening sentence of the first paragraph?

Multiple processing streams in occipitotemporal visual cortex Signup and view all the answers

What should have been colored differently in the Progress article about the ancient regulatory-protein family of WD-repeat proteins?

'WD-repeating units for the TUPI protein' Signup and view all the answers

When was the article 'The ancient regulatory-protein family of WD-repeat proteins' accepted?

23 September 1994 Signup and view all the answers

What is the title of reference 1 in the provided text?

'Hemoglobin and Myoglobin in Their Reactions with Ligands' Signup and view all the answers

'Biochemistry' is referenced in the text for which purpose?

'Comparison with spectroscopy and computation' Signup and view all the answers

Which reference provides information about TUPI correction?

'Rothberg, L. J., Roberson, M. & Jedju, T. M.' Signup and view all the answers

What is the main focus of the transient spectroscopic studies mentioned in the text?

Identifying structural changes in myoglobin Signup and view all the answers

What does an increase in the enthalpic barrier to ligand rebinding from the pockee lead to?

Decrease in the rate coefficient Signup and view all the answers

What provides a basis for structural interpretation of the complex changes seen in an important spectroscopic marker?

Relaxation of Mb*CO towards unligated structure Signup and view all the answers

Protein Photolysis and Spectroscopic Studies Quiz

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Questions and Answers

What is the main focus of the transient spectroscopic studies mentioned in the text?

What are the functionally important structural changes observed in the relaxation from Mb*CO to unligated myoglobin?

What does the relaxation of the proximal haem environment from Mb*CO to unligated myoglobin conceptually resemble?

What does an increase in the enthalpic barrier to ligand rebinding from the pockee lead to?

What provides a basis for structural interpretation of the complex changes seen in an important spectroscopic marker?

What determines the frequency and intensity of the Raman FeN'2 stretch mode?

What method was used to obtain snapshots of myoglobin before, during, and after its reaction with carbon monoxide?

What information can help to decide the most fruitful approaches to questions about dynamics?

What type of models can be made using detailed structural information about the unstable Mb*CO complex?

What is the goal of combining detailed structural information with the results of other approaches?

Which article has a misleading error introduced in the opening sentence of the first paragraph?

What should have been colored differently in the Progress article about the ancient regulatory-protein family of WD-repeat proteins?

When was the article 'The ancient regulatory-protein family of WD-repeat proteins' accepted?

What is the title of reference 1 in the provided text?

'Biochemistry' is referenced in the text for which purpose?

Which reference provides information about TUPI correction?

What is the main focus of the transient spectroscopic studies mentioned in the text?

What does an increase in the enthalpic barrier to ligand rebinding from the pockee lead to?

What provides a basis for structural interpretation of the complex changes seen in an important spectroscopic marker?

What method was used to obtain snapshots of myoglobin before, during, and after its reaction with carbon monoxide?

Which reference provides information about TUPI correction?

'Biochemistry' is referenced in the text for which purpose?

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