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Questions and Answers
What is the main focus of the transient spectroscopic studies mentioned in the text?
What is the main focus of the transient spectroscopic studies mentioned in the text?
- Identifying structural changes in the photolysed state of Mb*CO
- Exploring the enthalpic barrier to ligand rebinding from the pockee
- Comparing the frequency and intensity of the Raman FeN'2 stretch mode
- Investigating the dynamics of the protein during relaxation (correct)
What are the functionally important structural changes observed in the relaxation from Mb*CO to unligated myoglobin?
What are the functionally important structural changes observed in the relaxation from Mb*CO to unligated myoglobin?
- Compression and motion of the F helix towards its junction with the E helix
- Increase in the enthalpic barrier decreasing the rate coefficient and lowering ligand-binding affinity
- Increase in iron out-of-plane distance, decrease in Fe-proximal histidine bond length, and tilting of the proximal histidine (correct)
- Decrease in the enthalpic part of the barrier to ligand rebinding from the pockee
What does the relaxation of the proximal haem environment from Mb*CO to unligated myoglobin conceptually resemble?
What does the relaxation of the proximal haem environment from Mb*CO to unligated myoglobin conceptually resemble?
- Decrease in Fe-proximal histidine bond length
- Increase in the enthalpic barrier decreasing the rate coefficient
- Allosteric R--> T transition of haemoglobin (correct)
- Dramatic increase in the enthalpic part of the barrier to ligand rebinding
What does an increase in the enthalpic barrier to ligand rebinding from the pockee lead to?
What does an increase in the enthalpic barrier to ligand rebinding from the pockee lead to?
What provides a basis for structural interpretation of the complex changes seen in an important spectroscopic marker?
What provides a basis for structural interpretation of the complex changes seen in an important spectroscopic marker?
What determines the frequency and intensity of the Raman FeN'2 stretch mode?
What determines the frequency and intensity of the Raman FeN'2 stretch mode?
What method was used to obtain snapshots of myoglobin before, during, and after its reaction with carbon monoxide?
What method was used to obtain snapshots of myoglobin before, during, and after its reaction with carbon monoxide?
What information can help to decide the most fruitful approaches to questions about dynamics?
What information can help to decide the most fruitful approaches to questions about dynamics?
What type of models can be made using detailed structural information about the unstable Mb*CO complex?
What type of models can be made using detailed structural information about the unstable Mb*CO complex?
What is the goal of combining detailed structural information with the results of other approaches?
What is the goal of combining detailed structural information with the results of other approaches?
Which article has a misleading error introduced in the opening sentence of the first paragraph?
Which article has a misleading error introduced in the opening sentence of the first paragraph?
What should have been colored differently in the Progress article about the ancient regulatory-protein family of WD-repeat proteins?
What should have been colored differently in the Progress article about the ancient regulatory-protein family of WD-repeat proteins?
When was the article 'The ancient regulatory-protein family of WD-repeat proteins' accepted?
When was the article 'The ancient regulatory-protein family of WD-repeat proteins' accepted?
What is the title of reference 1 in the provided text?
What is the title of reference 1 in the provided text?
'Biochemistry' is referenced in the text for which purpose?
'Biochemistry' is referenced in the text for which purpose?
Which reference provides information about TUPI correction?
Which reference provides information about TUPI correction?
What is the main focus of the transient spectroscopic studies mentioned in the text?
What is the main focus of the transient spectroscopic studies mentioned in the text?
What does an increase in the enthalpic barrier to ligand rebinding from the pockee lead to?
What does an increase in the enthalpic barrier to ligand rebinding from the pockee lead to?
What provides a basis for structural interpretation of the complex changes seen in an important spectroscopic marker?
What provides a basis for structural interpretation of the complex changes seen in an important spectroscopic marker?
What method was used to obtain snapshots of myoglobin before, during, and after its reaction with carbon monoxide?
What method was used to obtain snapshots of myoglobin before, during, and after its reaction with carbon monoxide?
Which reference provides information about TUPI correction?
Which reference provides information about TUPI correction?
'Biochemistry' is referenced in the text for which purpose?
'Biochemistry' is referenced in the text for which purpose?
