14 Questions
What is the primary cause of aggregation of unfolded proteins like hGH?
Hydrophobic interactions between unfolded proteins
How does hGH counteract aggregation?
By forming complex structures resistant to aggregates through Zn binding sites + By preventing proteins from coming together using surfactants like polysorbate-20
What role do surfactants like polysorbate-20 play in preventing protein aggregation?
Occupying surfaces/interfaces, preventing hydrophobic interactions
Which amino acid residues are the principle sites of deamidation in alkaline pH for human growth hormone?
Asn-149
What is the consequence of degradation of human growth hormone leading to immunogenicity?
Immune response
Which amino acid residue is the major oxidative site for human growth hormone?
Methionine
What is the primary structure of human growth hormone?
Contains 191 amino acids
What can lead to aggregation of unfolded proteins like hGH?
Hydrophobic interactions
What role do surfactants like polysorbate-20 play in preventing protein aggregation?
Occupying surfaces/interfaces to prevent hydrophobic interactions
How does hGH counteract aggregation?
Zn binding sites form complex structures resistant to aggregate formation
What is the primary function of human growth hormone (hGH)?
Stimulating growth in pediatric patients and maintaining metabolic balance in adult patients
What is the consequence of oxidation of methionine residue in human growth hormone?
Formation of metsulfoxide, which does not affect the hormone's potency
Where does deamidated human growth hormone exist inside our bodies?
Pituitary gland in the brain
What is the primary concern related to the degradation of human growth hormone?
Immunogenicity leading to an immune response
Optimizing Protein Formulation Quiz: Test your knowledge on achieving deliverability, bioavailability, and stability of solid protein formulations. Explore the use of excipients, stabilizers, administration routes, and medical devices to address degradation issues and enhance potency. Get insights into overcoming challenges in protein drug development.
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