Protein Formulation Optimization Quiz
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Questions and Answers

What is the primary cause of aggregation of unfolded proteins like hGH?

  • Formation of complex structures resistant to aggregates
  • Hydrophobic interactions between unfolded proteins (correct)
  • Presence of Zn binding sites
  • Surfactant occupation of surfaces/interfaces

    • How does hGH counteract aggregation?

  • By forming complex structures resistant to aggregates through Zn binding sites + By preventing proteins from coming together using surfactants like polysorbate-20 (correct)
  • By exposing hydrophobic interior to promote aggregation
  • LOL
  • By interacting with solid formulations or liquid interfaces

    • What role do surfactants like polysorbate-20 play in preventing protein aggregation?

  • Exposing hydrophobic interior of proteins
  • Promoting hydrophobic interactions between unfolded proteins
  • Facilitating the formation of protein aggregates
  • Occupying surfaces/interfaces, preventing hydrophobic interactions (correct)

    • Which amino acid residues are the principle sites of deamidation in alkaline pH for human growth hormone?

    <p>Asn-149</p> Signup and view all the answers

    What is the consequence of degradation of human growth hormone leading to immunogenicity?

    <p>Immune response</p> Signup and view all the answers

    Which amino acid residue is the major oxidative site for human growth hormone?

    <p>Methionine</p> Signup and view all the answers

    What is the primary structure of human growth hormone?

    <p>Contains 191 amino acids</p> Signup and view all the answers

    What can lead to aggregation of unfolded proteins like hGH?

    <p>Hydrophobic interactions</p> Signup and view all the answers

    What role do surfactants like polysorbate-20 play in preventing protein aggregation?

    <p>Occupying surfaces/interfaces to prevent hydrophobic interactions</p> Signup and view all the answers

    How does hGH counteract aggregation?

    <p>Zn binding sites form complex structures resistant to aggregate formation</p> Signup and view all the answers

    What is the primary function of human growth hormone (hGH)?

    <p>Stimulating growth in pediatric patients and maintaining metabolic balance in adult patients</p> Signup and view all the answers

    What is the consequence of oxidation of methionine residue in human growth hormone?

    <p>Formation of metsulfoxide, which does not affect the hormone's potency</p> Signup and view all the answers

    Where does deamidated human growth hormone exist inside our bodies?

    <p>Pituitary gland in the brain</p> Signup and view all the answers

    What is the primary concern related to the degradation of human growth hormone?

    <p>Immunogenicity leading to an immune response</p> Signup and view all the answers

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