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Questions and Answers
What is the primary cause of aggregation of unfolded proteins like hGH?
What is the primary cause of aggregation of unfolded proteins like hGH?
- Formation of complex structures resistant to aggregates
- Hydrophobic interactions between unfolded proteins (correct)
- Presence of Zn binding sites
- Surfactant occupation of surfaces/interfaces
How does hGH counteract aggregation?
How does hGH counteract aggregation?
- By forming complex structures resistant to aggregates through Zn binding sites + By preventing proteins from coming together using surfactants like polysorbate-20 (correct)
- By exposing hydrophobic interior to promote aggregation
- LOL
- By interacting with solid formulations or liquid interfaces
What role do surfactants like polysorbate-20 play in preventing protein aggregation?
What role do surfactants like polysorbate-20 play in preventing protein aggregation?
- Exposing hydrophobic interior of proteins
- Promoting hydrophobic interactions between unfolded proteins
- Facilitating the formation of protein aggregates
- Occupying surfaces/interfaces, preventing hydrophobic interactions (correct)
Which amino acid residues are the principle sites of deamidation in alkaline pH for human growth hormone?
Which amino acid residues are the principle sites of deamidation in alkaline pH for human growth hormone?
What is the consequence of degradation of human growth hormone leading to immunogenicity?
What is the consequence of degradation of human growth hormone leading to immunogenicity?
Which amino acid residue is the major oxidative site for human growth hormone?
Which amino acid residue is the major oxidative site for human growth hormone?
What is the primary structure of human growth hormone?
What is the primary structure of human growth hormone?
What can lead to aggregation of unfolded proteins like hGH?
What can lead to aggregation of unfolded proteins like hGH?
What role do surfactants like polysorbate-20 play in preventing protein aggregation?
What role do surfactants like polysorbate-20 play in preventing protein aggregation?
How does hGH counteract aggregation?
How does hGH counteract aggregation?
What is the primary function of human growth hormone (hGH)?
What is the primary function of human growth hormone (hGH)?
What is the consequence of oxidation of methionine residue in human growth hormone?
What is the consequence of oxidation of methionine residue in human growth hormone?
Where does deamidated human growth hormone exist inside our bodies?
Where does deamidated human growth hormone exist inside our bodies?
What is the primary concern related to the degradation of human growth hormone?
What is the primary concern related to the degradation of human growth hormone?
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