Podcast
Questions and Answers
What type of gel is best to resolve a mixture of X, Y, and Z on a denaturing gel?
What type of gel is best to resolve a mixture of X, Y, and Z on a denaturing gel?
- Both
- Denaturing (correct)
- idk
- Non-denaturing
In electrophoresis, which factor can affect migration but was not discussed in the text?
In electrophoresis, which factor can affect migration but was not discussed in the text?
- Size of the gel
- Angle of applied current
- Material of the gel
- Buffers used
- All of the above affect migration (correct)
Which type of gel is most suitable to determine if a protein is binding to a regulatory molecule in a sample?
Which type of gel is most suitable to determine if a protein is binding to a regulatory molecule in a sample?
- Denaturing
- Non-denaturing (correct)
- idk
- Both
When comparing two samples to check for the presence of a specific protein of known molecular weight, what type of gel should be used?
When comparing two samples to check for the presence of a specific protein of known molecular weight, what type of gel should be used?
What would you expect to see when running a mixture of X, Y, and Z on a denaturing gel?
What would you expect to see when running a mixture of X, Y, and Z on a denaturing gel?
Which type of gel resolves samples based on structure/function in addition to size and charge?
Which type of gel resolves samples based on structure/function in addition to size and charge?
What is the primary basis for resolving molecules in denaturing electrophoresis of proteins?
What is the primary basis for resolving molecules in denaturing electrophoresis of proteins?
Why is it important to understand the nuances of electrophoresis beyond just 'separating by size'?
Why is it important to understand the nuances of electrophoresis beyond just 'separating by size'?
In denaturing electrophoresis, what happens to the proteins prior to loading them onto the gel?
In denaturing electrophoresis, what happens to the proteins prior to loading them onto the gel?
What aspect of molecules is not considered when someone refers to 'size' in electrophoresis?
What aspect of molecules is not considered when someone refers to 'size' in electrophoresis?
What is a key factor that affects migration in electrophoresis besides molecular weight and charge?
What is a key factor that affects migration in electrophoresis besides molecular weight and charge?
What is the main factor that affects migration through a gel in electrophoresis?
What is the main factor that affects migration through a gel in electrophoresis?
Why is precise descriptor important in disciplinary practice related to gel electrophoresis?
Why is precise descriptor important in disciplinary practice related to gel electrophoresis?
In the context of the Taura syndrome virus proteins X, Y, and Z, which combination of proteins would likely show up as three distinct bands on a gel?
In the context of the Taura syndrome virus proteins X, Y, and Z, which combination of proteins would likely show up as three distinct bands on a gel?
If a protein exists in two distinct conformations in a cell, and you run it on a gel, how would the gel appear if your idea is true?
If a protein exists in two distinct conformations in a cell, and you run it on a gel, how would the gel appear if your idea is true?
What parameter should be considered to deduce unknown molecules' properties from their migration through a gel?
What parameter should be considered to deduce unknown molecules' properties from their migration through a gel?
If you suspect that a protein binds to other proteins Y and Z, what would you expect to observe in a gel electrophoresis experiment?
If you suspect that a protein binds to other proteins Y and Z, what would you expect to observe in a gel electrophoresis experiment?
Which of the following proteins would migrate the furthest in an agarose gel electrophoresis setup?
Which of the following proteins would migrate the furthest in an agarose gel electrophoresis setup?
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