Podcast
Questions and Answers
Which term best describes protein crystals?
Which term best describes protein crystals?
- Amorphous
- Irregular
- Random
- Regular (correct)
What are protein crystals made of?
What are protein crystals made of?
- Identical molecules or molecular complexes (correct)
- Random mixtures
- Different molecules
- Various compounds
What is the structure of protein crystals?
What is the structure of protein crystals?
- Amorphous
- Irregular
- Regular (correct)
- Random
Which of the following can act as a nucleus in the nucleation process?
Which of the following can act as a nucleus in the nucleation process?
What happens if the supersaturation is too high in a solution?
What happens if the supersaturation is too high in a solution?
When does crystal growth often occur in supersaturated solutions?
When does crystal growth often occur in supersaturated solutions?
Which of the following can cause cessation of growth in crystal formation?
Which of the following can cause cessation of growth in crystal formation?
What is mother liquor in the context of crystal formation?
What is mother liquor in the context of crystal formation?
How is mother liquor different from the original crystallization screening solution?
How is mother liquor different from the original crystallization screening solution?
What is the role of temperature in crystallization?
What is the role of temperature in crystallization?
Which method is commonly used by crystallographers to obtain protein?
Which method is commonly used by crystallographers to obtain protein?
What can cause low expression yields in E. Coli expression systems?
What can cause low expression yields in E. Coli expression systems?
What is a major issue with protein concentration?
What is a major issue with protein concentration?
Which temperature is considered ideal for keeping an individual crystal screen constant?
Which temperature is considered ideal for keeping an individual crystal screen constant?
At which temperatures should each set of conditions be screened during crystal formation?
At which temperatures should each set of conditions be screened during crystal formation?
Which temperature should be tried during crystal screening, in addition to 4° C and room temperature?
Which temperature should be tried during crystal screening, in addition to 4° C and room temperature?
Which type of interactions are more important than hydrophobic interactions in crystal packing?
Which type of interactions are more important than hydrophobic interactions in crystal packing?
What does crystal packing refer to?
What does crystal packing refer to?
What is the main issue when it comes to reproducibility in crystal packing?
What is the main issue when it comes to reproducibility in crystal packing?
Which temperature is commonly used for X-ray diffraction measurements of protein crystals?
Which temperature is commonly used for X-ray diffraction measurements of protein crystals?
What is the purpose of adding cryoprotectants to protein crystals?
What is the purpose of adding cryoprotectants to protein crystals?
What effect does cryoprotectant addition have on the nucleation temperature of water?
What effect does cryoprotectant addition have on the nucleation temperature of water?
Why can't we see proteins with light microscopes?
Why can't we see proteins with light microscopes?
What is the wavelength range of visible light?
What is the wavelength range of visible light?
What is the wavelength of X-rays?
What is the wavelength of X-rays?
Which type of device is used to force electrons to follow a closed 'ring' trajectory?
Which type of device is used to force electrons to follow a closed 'ring' trajectory?
What is the main difference between a wiggler and an undulator?
What is the main difference between a wiggler and an undulator?
What is the purpose of insertion devices in synchrotron radiation facilities?
What is the purpose of insertion devices in synchrotron radiation facilities?