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Questions and Answers
Which group of amino acids contains an ionizable side chain?
Which group of amino acids contains an ionizable side chain?
- Histidine (correct)
- Leucine
- Alanine
- Glycine
What is the purpose of adding ammonium sulfate to a solution of proteins?
What is the purpose of adding ammonium sulfate to a solution of proteins?
- To purify the solution
- To precipitate the target protein
- To remove the less soluble impurities (correct)
- To exchange the solvent
What is the role of dialysis in protein purification?
What is the role of dialysis in protein purification?
- To exchange the solvent (correct)
- To precipitate the target protein
- To remove the less soluble impurities
- To purify the solution
What is the purpose of dissolving the protein precipitate in a minimal volume of buffer solution?
What is the purpose of dissolving the protein precipitate in a minimal volume of buffer solution?
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What does mass spectrometry measure?
What does mass spectrometry measure?
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Which group of amino acids contains a weaker acid than the -COOH group?
Which group of amino acids contains a weaker acid than the -COOH group?
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What is the purpose of mixing enough ammonium sulfate with the protein solution?
What is the purpose of mixing enough ammonium sulfate with the protein solution?
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What happens to the less soluble impurities after centrifugation?
What happens to the less soluble impurities after centrifugation?
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What is the purpose of adding more ammonium sulfate to the supernatant fraction?
What is the purpose of adding more ammonium sulfate to the supernatant fraction?
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What is the purpose of dialysis in protein purification?
What is the purpose of dialysis in protein purification?
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