10 Questions
Which group of amino acids contains an ionizable side chain?
Histidine
What is the purpose of adding ammonium sulfate to a solution of proteins?
To remove the less soluble impurities
What is the role of dialysis in protein purification?
To exchange the solvent
What is the purpose of dissolving the protein precipitate in a minimal volume of buffer solution?
To concentrate the protein
What does mass spectrometry measure?
The time
Which group of amino acids contains a weaker acid than the -COOH group?
NH4+
What is the purpose of mixing enough ammonium sulfate with the protein solution?
To precipitate the less soluble impurities
What happens to the less soluble impurities after centrifugation?
They remain in the fluid
What is the purpose of adding more ammonium sulfate to the supernatant fraction?
To precipitate the target protein
What is the purpose of dialysis in protein purification?
To exchange residual ammonium sulfate
This quiz covers the classification and purification of proteins. Learn about the different titratable groups of alanine and the ionizable side chain of histidine. Test your knowledge on protein chemistry.
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