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Protein Classification and Purification Quiz

10 Questions

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Protein Classification and Purification Quiz

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Questions and Answers

Which group of amino acids contains an ionizable side chain?

Histidine (correct)

Leucine

Alanine

Glycine

What is the purpose of adding ammonium sulfate to a solution of proteins?

To purify the solution

To precipitate the target protein

To remove the less soluble impurities (correct)

To exchange the solvent

What is the role of dialysis in protein purification?

To exchange the solvent (correct)

To precipitate the target protein

To remove the less soluble impurities

To purify the solution

What is the purpose of dissolving the protein precipitate in a minimal volume of buffer solution?

To concentrate the protein Signup and view all the answers

What does mass spectrometry measure?

The time Signup and view all the answers

Which group of amino acids contains a weaker acid than the -COOH group?

NH4+ Signup and view all the answers

What is the purpose of mixing enough ammonium sulfate with the protein solution?

To precipitate the less soluble impurities Signup and view all the answers

What happens to the less soluble impurities after centrifugation?

They remain in the fluid Signup and view all the answers

What is the purpose of adding more ammonium sulfate to the supernatant fraction?

To precipitate the target protein Signup and view all the answers

What is the purpose of dialysis in protein purification?

To exchange residual ammonium sulfate Signup and view all the answers