Protein Biosynthesis Process

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Questions and Answers

What is the primary function of the 5' cap added to pre-mRNA?

  • To bind to ribosomes for protein synthesis (correct)
  • To facilitate the removal of introns
  • To add adenine bases to the mRNA
  • To prevent degradation of the mRNA (correct)

Which of the following accurately describes introns?

  • They are essential for protein translation.
  • They are modifications added to mRNA.
  • They encode proteins directly.
  • They are non-coding sequences in genes. (correct)

How many adenine bases typically comprise the 3' poly(A) tail added to mRNA?

  • 200-300 bases
  • 50-100 bases
  • 100-200 bases (correct)
  • 300-400 bases

What is the role of RNA splicing in the maturation of pre-mRNA?

<p>To remove introns from the pre-mRNA (B)</p> Signup and view all the answers

Which characteristic is essential for the detection of a complete mRNA message?

<p>Presence of both the 5' cap and 3' tail (B)</p> Signup and view all the answers

What role do ribosomes play in protein synthesis?

<p>They catalyze the formation of covalent peptide bonds. (D)</p> Signup and view all the answers

What must a polypeptide chain do after translation to become a functional protein?

<p>Fold into a functional three-dimensional shape. (A)</p> Signup and view all the answers

Which structure forms first during the folding of a polypeptide chain?

<p>Secondary structure (A)</p> Signup and view all the answers

What effect can post-translational modifications have on a protein?

<p>They can alter the protein's function and localization. (C)</p> Signup and view all the answers

How can mutations in DNA affect protein production?

<p>They can alter the mRNA encoded amino acid sequence. (A)</p> Signup and view all the answers

What direction does RNA polymerase read the template strand during mRNA synthesis?

<p>3' to 5' (B)</p> Signup and view all the answers

What can result from a nonsense mutation?

<p>Early termination of translation (D)</p> Signup and view all the answers

What is the tertiary structure of a protein?

<p>The overall three-dimensional shape of the protein (A)</p> Signup and view all the answers

What type of bond does RNA polymerase catalyze to build the pre-mRNA molecule?

<p>Phosphodiester bonds (D)</p> Signup and view all the answers

What can be a consequence of protein misfolding?

<p>Potential involvement in disease (C)</p> Signup and view all the answers

How does RNA polymerase handle incorrect nucleotides during pre-mRNA synthesis?

<p>It removes them using an excision reaction. (D)</p> Signup and view all the answers

What happens when RNA polymerase reaches a specific termination sequence?

<p>RNA polymerase detaches, completing pre-mRNA synthesis. (A)</p> Signup and view all the answers

Which of the following bases is found in RNA but not in DNA?

<p>Uracil (D)</p> Signup and view all the answers

What is the nucleotide incorporation rate of RNA polymerase during mRNA synthesis?

<p>20 nucleotides per second (B)</p> Signup and view all the answers

Which of these statements about the synthesized pre-mRNA is correct?

<p>It is complementary to the coding DNA strand. (C)</p> Signup and view all the answers

What is the maximum number of base pairs exposed to RNA polymerase at one time during transcription?

<p>12 base pairs (B)</p> Signup and view all the answers

What is the role of the spliceosome in the maturation of mRNA?

<p>To remove introns from pre-mRNA (A)</p> Signup and view all the answers

Where does translation occur in eukaryotic cells?

<p>In the cytoplasm (D)</p> Signup and view all the answers

Which molecule is primarily responsible for delivering amino acids to the ribosome during translation?

<p>tRNA (C)</p> Signup and view all the answers

How does a ribosome read the mRNA template during translation?

<p>In triplets of nucleotides (D)</p> Signup and view all the answers

What initiates the translation process at the ribosome?

<p>The recognition of the start codon (D)</p> Signup and view all the answers

What structural component forms the ribosome?

<p>A mix of protein and ribosomal RNA (D)</p> Signup and view all the answers

What is the function of codons in the mRNA sequence?

<p>To code for specific amino acids (C)</p> Signup and view all the answers

Which of the following correctly describes the nature of tRNA?

<p>tRNA has a characteristic cloverleaf structure (A)</p> Signup and view all the answers

What is the final structure that a polypeptide adopts after folding?

<p>Mature functional 3D state (B)</p> Signup and view all the answers

Which of the following is NOT a type of post-translational modification?

<p>Formation of peptide bonds (A)</p> Signup and view all the answers

What is the role of proteases in protein processing?

<p>They irreversibly cleave proteins (B)</p> Signup and view all the answers

How do post-translational modifications impact proteins?

<p>They can alter protein activity and interactions (C)</p> Signup and view all the answers

Which of the following statements about cleavage as a post-translational modification is true?

<p>It results in a permanently shortened protein. (B)</p> Signup and view all the answers

What is the potential impact of the addition of complex molecules as a post-translational modification?

<p>It can expand the functionality and diversity of proteins. (C)</p> Signup and view all the answers

Why are there so many types of post-translational modifications?

<p>They collectively expand protein diversity encoded by the genome. (C)</p> Signup and view all the answers

What may result from the cleavage of a polypeptide chain?

<p>Change in the starting and ending amino acids (C)</p> Signup and view all the answers

Which of the following processes is NOT commonly associated with post-translational modifications?

<p>Glycoprotein synthesis (A)</p> Signup and view all the answers

What is the role of glycosylation in post-translational modifications?

<p>To facilitate the folding of proteins (D)</p> Signup and view all the answers

Which amino acids are most commonly involved in the formation of disulfide bonds?

<p>Cysteine and cysteine (A)</p> Signup and view all the answers

Which type of enzyme is responsible for adding a polysaccharide molecule during glycosylation?

<p>Transferases (B)</p> Signup and view all the answers

What happens during the phosphorylation process in proteins?

<p>Incorporation of a phosphate group (C)</p> Signup and view all the answers

Acetylation as a post-translational modification primarily affects which functional group?

<p>Amine group (C)</p> Signup and view all the answers

Which post-translational modification can directly influence the interaction of proteins with other molecules?

<p>Both B and C (B)</p> Signup and view all the answers

Where in the cell does the modification of glycosylation primarily occur?

<p>Endoplasmic reticulum and Golgi apparatus (C)</p> Signup and view all the answers

Flashcards

Translation

The process of forming a polypeptide chain from amino acids based on the sequence in mRNA.

Active site

The site on an enzyme where the substrate binds and the chemical reaction takes place.

Tertiary structure

The three-dimensional arrangement of a polypeptide chain, resulting from interactions between amino acids.

Post-translational modifications

Changes or modifications made to a protein after it has been synthesized.

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Secondary structure

The specific three-dimensional arrangement of amino acids within a polypeptide chain, including alpha-helices and beta-sheets.

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DNA mutation

A change in the DNA sequence.

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Nonsense mutation

A mutation that introduces a stop codon, resulting in the premature termination of protein synthesis.

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Missense mutation

A mutation that changes the amino acid encoded by a codon.

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Post-transcriptional Modification

Process of modifying pre-mRNA into mature mRNA after transcription.

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5' cap

A protective cap added to the 5' end of mRNA, consisting of a modified guanine nucleotide.

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Poly(A) tail

A long sequence of adenine nucleotides added to the 3' end of mRNA, protecting it from degradation.

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Introns

Non-coding sequences within a gene that are removed from pre-mRNA during splicing.

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Exons

Coding sequences within a gene that are joined together to form mature mRNA.

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RNA polymerase

An enzyme responsible for synthesizing pre-mRNA by reading the template DNA strand.

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Phosphodiester bonds

The chemical bonds that link nucleotides together in RNA and DNA. In mRNA synthesis, RNA polymerase forms phosphodiester bonds.

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Base pairing

The pairing of complementary nitrogenous bases in DNA and RNA. Adenine pairs with thymine (in DNA) or uracil (in RNA), and guanine pairs with cytosine.

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Proofreading (RNA polymerase)

A mechanism that allows RNA polymerase to remove incorrect nucleotides from the growing pre-mRNA molecule, ensuring accuracy.

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Termination signal (transcription)

A specific DNA sequence that signals the end of transcription, causing RNA polymerase to detach and release the pre-mRNA.

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Bases in RNA

RNA is composed of the bases guanine, cytosine, adenine, and uracil.

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Pre-mRNA

A single-stranded copy of a gene that is synthesized during transcription, and will undergo further processing before translation.

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Gene

A sequence of DNA that encodes for a specific protein, and is transcribed into pre-mRNA.

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Ribosome

A specialized molecular complex that 'reads' mRNA and assembles amino acids into a polypeptide chain.

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Transfer RNA (tRNA)

Small RNA molecules that carry specific amino acids to the ribosome, matching them to the codons in mRNA.

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Codon

A three-nucleotide sequence in mRNA that specifies a particular amino acid.

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Start codon

The specific codon that signals the start of translation.

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Splicing

The process of removing introns and joining exons within a pre-mRNA molecule to create a mature mRNA molecule.

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Central dogma of molecular biology

The flow of genetic information from DNA to RNA to protein.

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Protein Folding

The specific three-dimensional shape a polypeptide chain folds into, forming a functional protein. This structure is essential for a protein's function and is influenced by interactions between amino acids.

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Cleavage

The irreversible cutting of a polypeptide chain by enzymes called proteases. This often alters the protein's function, either activating or inactivating it.

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Proteases

Enzymes that specifically break down peptide bonds within a protein, leading to cleavage.

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Hydrolysis

A chemical reaction where a molecule is broken down by adding water.

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Types of Post-translational Modifications

The addition of chemical groups, complex molecules, or the formation of bonds within a protein after its initial synthesis.

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Polypeptide Chain

The initial linear chain of amino acids, formed during translation. It represents the primary structure of a protein.

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Methylation

Adding a methyl group (CH3) to a protein's side chain. It's like adding a tiny tag to modify a protein's behavior.

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Acetylation

Adding an acetyl group (CH3CO) to a protein's side chain. It's like attaching a small chemical switch to modify a protein's activity.

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Phosphorylation

Adding a phosphate group (PO4) to a protein's side chain. It's like adding a key that can turn a protein's activity on or off.

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Glycosylation

Adding a sugar molecule (polysaccharide) to a protein's side chain. It's like decorating a protein with a sugary frosting.

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Polysaccharide

A long chain of sugar molecules. It's like a string of beads made of sugar.

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Glycan

A specific sugar molecule that is attached to a protein during glycosylation. It's like a small, sugary ornament.

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Glycosyltransferase

A type of enzyme that attaches sugars to proteins during glycosylation. It's like a sugar-adding machine.

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Glycosidase

A type of enzyme that modifies sugars after glycosylation. It's like a sugar-editor.

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Study Notes

Protein Biosynthesis

  • Protein biosynthesis, also known as protein synthesis, is a crucial cellular process. It maintains cellular protein levels by producing new proteins and balancing loss from degradation or export.
  • This process is broadly divided into two key phases: transcription and translation.

Transcription

  • During transcription, a segment of DNA, called a gene, is copied into a template molecule called messenger RNA (mRNA).
  • This process occurs within the nucleus of a cell.
  • RNA polymerase enzymes carry out this conversion.
  • A single strand of pre-mRNA is produced, complementary to the DNA template strand.

Post-transcriptional modifications

  • Pre-mRNA undergoes alterations to become mature mRNA before export into cytoplasm.
  • 5' cap addition: The 5' end of pre-mRNA is modified with a guanine nucleotide cap to prevent degradation and aid ribosome binding.
  • 3' poly(A) tail addition: A string of adenine nucleotides is added to the 3' end to stabilize mRNA and enable its export from the nucleus.
  • RNA splicing: Introns (non-coding regions) are removed from the pre-mRNA molecule, and exons (coding regions) are joined together by spliceosomes, creating a mature mRNA molecule.

Translation

  • In the cytoplasm, the ribosomes read the mature mRNA sequence.
  • Ribosomes use transfer RNA (tRNA) molecules to bring the correct amino acids to the ribosome that corresponds with the mRNA codons.
  • Covalent peptide bonds are formed between the amino acids, creating a polypeptide chain.
  • This process continues until a stop codon is reached.
  • Polypeptides then fold into their three-dimensional (3D) structures.

Protein Folding

  • Polypeptide chains fold to form functional proteins.
  • Secondary, tertiary, and quaternary structures determine function.
  • Protein folding is crucial for function.

Post-translational modifications

  • After translation, proteins may undergo further modification to become fully functional proteins.
  • These modifications may involve cleavage, addition of chemical groups like methylation, acetylation, or phosphorylation.
  • Glycosylation is another common modification, adding polysaccharide chains.

Errors in Proteins

  • Mutations in DNA can cause changes in amino acid sequences which can alter protein function or folding.
  • Mutations can lead to misfolding, which can cause diseases such as Alzheimer's and Parkinson's.
  • Errors in protein biosynthesis and folding have a role in various diseases.

Termination

  • RNA polymerase stops transcribing mRNA at defined termination points in DNA.
  • This ends protein synthesis after a stop codon (e.g. UAA, UAG,or UGA)

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