Protein Analysis and Purification Quiz
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Protein Analysis and Purification Quiz

Created by
@DazzlingFreedom

Questions and Answers

Which method is generally used to test the function of a protein in vivo?

MTX selection

What makes cells resistant to Methotrexate (MTX)?

Stable gene integration

Which type of proteins are best expressed using eukaryotic expression systems?

Large proteins (>100kD)

What is the purpose of stable gene integration in protein expression?

<p>To integrate genes into random locations within different chromosomes</p> Signup and view all the answers

Which method selects for cells with the highest expression of the gene of interest (GOI) and DHFR gene?

<p>MTX selection</p> Signup and view all the answers

Which method is commonly used to quantitate proteins in a protein extract?

<p>A280</p> Signup and view all the answers

Which expression system is optimal for protein expression?

<p>Mammalian cell line</p> Signup and view all the answers

Which expression system is commonly used for expressing large proteins?

<p>Yeast cells</p> Signup and view all the answers

Which expression system is capable of performing glycosylation?

<p>Mammalian cell line</p> Signup and view all the answers

Which system is commonly used for expressing difficult-to-express proteins with low risk?

<p>Baculovirus</p> Signup and view all the answers

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