Questions and Answers
Which method is generally used to test the function of a protein in vivo?
MTX selection
What makes cells resistant to Methotrexate (MTX)?
Stable gene integration
Which type of proteins are best expressed using eukaryotic expression systems?
Large proteins (>100kD)
What is the purpose of stable gene integration in protein expression?
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Which method selects for cells with the highest expression of the gene of interest (GOI) and DHFR gene?
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Which method is commonly used to quantitate proteins in a protein extract?
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Which expression system is optimal for protein expression?
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Which expression system is commonly used for expressing large proteins?
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Which expression system is capable of performing glycosylation?
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Which system is commonly used for expressing difficult-to-express proteins with low risk?
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