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Questions and Answers
Which method is generally used to test the function of a protein in vivo?
Which method is generally used to test the function of a protein in vivo?
- Stable gene integration
- MTX selection (correct)
- Electroporation
- Protein expression
What makes cells resistant to Methotrexate (MTX)?
What makes cells resistant to Methotrexate (MTX)?
- Stable gene integration (correct)
- MTX selection
- Electroporation
- Protein expression
Which type of proteins are best expressed using eukaryotic expression systems?
Which type of proteins are best expressed using eukaryotic expression systems?
- Large proteins (>100kD) (correct)
- Small proteins
- Proteins expressed in multiple rounds
- Proteins expressed in increasing MTX levels
What is the purpose of stable gene integration in protein expression?
What is the purpose of stable gene integration in protein expression?
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Which method selects for cells with the highest expression of the gene of interest (GOI) and DHFR gene?
Which method selects for cells with the highest expression of the gene of interest (GOI) and DHFR gene?
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Which method is commonly used to quantitate proteins in a protein extract?
Which method is commonly used to quantitate proteins in a protein extract?
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Which expression system is optimal for protein expression?
Which expression system is optimal for protein expression?
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Which expression system is commonly used for expressing large proteins?
Which expression system is commonly used for expressing large proteins?
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Which expression system is capable of performing glycosylation?
Which expression system is capable of performing glycosylation?
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Which system is commonly used for expressing difficult-to-express proteins with low risk?
Which system is commonly used for expressing difficult-to-express proteins with low risk?
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