Protein Analysis and Purification Quiz
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Questions and Answers

Which method is generally used to test the function of a protein in vivo?

  • Stable gene integration
  • MTX selection (correct)
  • Electroporation
  • Protein expression
  • What makes cells resistant to Methotrexate (MTX)?

  • Stable gene integration (correct)
  • MTX selection
  • Electroporation
  • Protein expression
  • Which type of proteins are best expressed using eukaryotic expression systems?

  • Large proteins (>100kD) (correct)
  • Small proteins
  • Proteins expressed in multiple rounds
  • Proteins expressed in increasing MTX levels
  • What is the purpose of stable gene integration in protein expression?

    <p>To integrate genes into random locations within different chromosomes</p> Signup and view all the answers

    Which method selects for cells with the highest expression of the gene of interest (GOI) and DHFR gene?

    <p>MTX selection</p> Signup and view all the answers

    Which method is commonly used to quantitate proteins in a protein extract?

    <p>A280</p> Signup and view all the answers

    Which expression system is optimal for protein expression?

    <p>Mammalian cell line</p> Signup and view all the answers

    Which expression system is commonly used for expressing large proteins?

    <p>Yeast cells</p> Signup and view all the answers

    Which expression system is capable of performing glycosylation?

    <p>Mammalian cell line</p> Signup and view all the answers

    Which system is commonly used for expressing difficult-to-express proteins with low risk?

    <p>Baculovirus</p> Signup and view all the answers

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