Prokaryotic vs. Eukaryotic Cells
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Questions and Answers

What key characteristic defines a lytic cycle in bacteriophages?

  • The cycle is initiated by UV light
  • Progeny virus particles are produced rapidly (correct)
  • Phage genome integrates into the host genome
  • The cycle does not harm the host cell

Which factor can induce a lysogenic cycle to transition into a lytic cycle?

  • Presence of a ghost phage
  • Lower activation energy
  • Integration of phage genome into host genome
  • Exposure to UV light (correct)

During which stage of the lytic cycle does the assembly of new virions occur?

  • Maturation (correct)
  • Penetration
  • Release
  • Adsorption

How do enzymes primarily speed up chemical reactions?

<p>Lowering the activation energy (D)</p> Signup and view all the answers

What happens to the enzyme once the reaction is catalyzed?

<p>It remains unchanged and can bind additional substrates (D)</p> Signup and view all the answers

What is the role of a prophage in the lysogenic cycle?

<p>Integrates with bacterial chromosome, forming a prophage (D)</p> Signup and view all the answers

Which model suggests that the enzyme does not change shape upon substrate binding?

<p>Lock and key model (B)</p> Signup and view all the answers

What characteristic is shared by all prokaryotic cells?

<p>Circular DNA (B)</p> Signup and view all the answers

Which cellular structure is larger in eukaryotes compared to prokaryotes?

<p>Ribosomes (B)</p> Signup and view all the answers

What feature is unique to the lysogenic cycle and not found in the lytic cycle of bacteriophages?

<p>Phage genome integration into host DNA (D)</p> Signup and view all the answers

Which cell type divides using binary fission?

<p>Prokaryotic cells (D)</p> Signup and view all the answers

In which type of organism would you find cells with linear and complex DNA?

<p>Plants (C)</p> Signup and view all the answers

What is a characteristic of the lytic cycle of bacteriophages?

<p>No reliance on host DNA for replication (D)</p> Signup and view all the answers

What is a function of the cytoskeleton in eukaryotic cells?

<p>Providing structural support (D)</p> Signup and view all the answers

Which of the following organisms contains only unicellular structures?

<p>Bacteria (D)</p> Signup and view all the answers

Flashcards

Lytic Cycle

A viral reproductive cycle leading to quick production of progeny virus particles and host cell lysis.

Lysogenic Cycle

A viral reproductive cycle where the phage genome integrates into the host genome without immediate harm to the host.

Prophage

The integrated viral genome within the host bacterial chromosome in the lysogenic cycle.

Lysogen

A bacterium that carries a prophage in its genome, which can affect its traits.

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Activation Energy

The minimum energy required for a chemical reaction to occur.

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Enzyme-Substrate Complex

The temporary complex formed when a substrate binds to the active site of an enzyme.

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Induced Fit Model

A model describing how enzymes change shape to bind substrates more closely after initial contact.

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Prokaryotes

Cells without a nucleus and organelles, including bacteria and archaea.

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Eukaryotes

Cells with a nucleus and membrane-bound organelles, part of multicellular organisms.

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Bacteriophages

Viruses that specifically infect bacteria; composed of a capsid, tail sheath, and fibers.

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Cell Wall

A rigid structure surrounding the cell membrane in many prokaryotes and some eukaryotes.

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Binary Fission

A method of asexual reproduction in prokaryotes, where a cell divides into two identical cells.

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Ribosomes

Cellular structures that synthesize proteins, found in both prokaryotic and eukaryotic cells.

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Study Notes

Prokaryotic vs. Eukaryotic Cells

  • Prokaryotic cells lack a nucleus and membrane-bound organelles.

  • Examples include bacteria and archaea.

  • Size: 0.1 micrometers to 5 micrometers.

  • Unicellular only.

  • DNA is circular.

  • 70S ribosomes.

  • Cytoskeleton made of analogous proteins.

  • Reproduce asexually via binary fission.

  • Cell wall in all prokaryotes

  • Eukaryotic cells have a nucleus and membrane-bound organelles.

  • Larger than prokaryotic cells (10-100 micrometers).

  • Can be unicellular or multicellular.

  • Examples include plants, animals, and fungi.

  • DNA is linear and complex.

  • 80S ribosomes.

  • Cytoskeleton made of actin, intermediate filaments, and microtubules.

  • Reproduce sexually or asexually via mitosis.

  • Cell walls found in plants and fungi.

  • Both types of cells:

    • Contain DNA as genetic material
    • Contain cytoplasm
    • Use ribosomes in protein synthesis
    • Have a cell membrane made of phospholipids

Viral Replication Cycles

  • Viruses are acellular particles, non-living until they invade a host.
  • Bacteriophages are viruses that infect bacteria only.
  • Structure: capsid head (nucleic acid), tail sheath, tail fibers.
  • Two replication cycles: lytic and lysogenic.

Lytic Cycle

  • Temperate/non-virulent phages.
  • Kills host cell through lysis.
  • No prophage stage.
  • Phage replication independent of host DNA.
  • Complete takeover of host cell mechanisms.
  • High phage genome productivity.
  • Fast process; progeny virions released quickly.
  • No lysogenic cycle transition.

Lysogenic Cycle

  • Virulent phages.

  • Does not harm the host cell initially.

  • Phage DNA integrates into host genome (prophage).

  • Replication of prophage along with host DNA.

  • Does not take complete control of host mechanisms.

  • Low phage genome productivity.

  • Slow process; no progeny virions released immediately.

  • Can transition to lytic cycle under certain conditions (UV, lack of nutrients, chemicals).

  • Five steps of both cycles (Lytic): adsorption, penetration, replication, maturation, lysis/release

  • Ghost refers to empty phage particle remaining outside bacteria after lysis/release.

  • Lysogenic conversion: prophage induces phenotypic changes in the host.

Enzymes

  • Enzymes are specialized proteins that speed up chemical reactions.
  • They lower the activation energy (minimum energy needed for reaction).
  • Do not change the overall energy of the reaction.
  • Enzymes bind to substrates at the active site.
  • Enzyme-substrate complex forms (lock-and-key or induced fit model).
  • Lock-and-key: enzyme and substrate fit perfectly.
  • Induced fit: enzyme changes shape to fit the substrate.
  • Product forms and is released; enzyme is unchanged and can bind more substrates.

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Explore the fundamental differences between prokaryotic and eukaryotic cells in this quiz. Learn about their structure, functions, and reproduction methods. Test your understanding of cell biology concepts and definitions.

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