Prokaryotic Structure and Cell Wall

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Questions and Answers

What is the primary function of the phospholipid bilayer in a prokaryotic cell membrane?

  • To generate energy using the proton motive force.
  • To synthesize proteins for cell function.
  • To act as a semi-permeable barrier, regulating the passage of substances. (correct)
  • To facilitate the transport of all molecules into and out of the cell.

Which of the following molecules can typically diffuse directly through the cell membrane due to passive diffusion?

  • Large proteins
  • Oxygen gas ($O_2$) (correct)
  • Glucose molecules
  • Charged Ions

What is the proton motive force (PMF) primarily used for in prokaryotic cells?

  • Digesting large macromolecules
  • Regulating cell size through osmosis
  • Synthesizing the cell wall
  • Producing ATP for cellular energy (correct)

In a hypertonic environment, what will be the net movement of water in relation to a prokaryotic cell?

<p>Water will move out of the cell. (B)</p> Signup and view all the answers

Which cellular component is NOT a primary structural feature of prokaryotic cells?

<p>Nucleus (C)</p> Signup and view all the answers

Which of the following structures is NOT found in all prokaryotic species?

<p>Glycogen inclusions (B)</p> Signup and view all the answers

What is the primary function of pili or fimbriae in bacteria?

<p>Attachment to surfaces and other cells (B)</p> Signup and view all the answers

Which bacterial genus is NOT known for forming endospores?

<p>Escherichia (A)</p> Signup and view all the answers

What is the energy source for flagellar rotation in bacteria?

<p>Proton motive force (PMF) (B)</p> Signup and view all the answers

Which photosynthetic bacteria uses bacteriochlorophyll and does NOT produce oxygen?

<p>Green sulfur bacteria (B)</p> Signup and view all the answers

What is the primary purpose of bacterial storage inclusions?

<p>Storage of various materials (D)</p> Signup and view all the answers

Which of the following is a characteristic trait of bacterial endospores?

<p>Extreme resistance to harsh conditions (B)</p> Signup and view all the answers

Which of these describes the membrane found within a cyanobacteria?

<p>Thylakoid membrane (D)</p> Signup and view all the answers

What is the primary role of lipopolysaccharide (LPS) in the context of bacterial infections?

<p>To trigger an inflammatory response and potentially septic shock. (A)</p> Signup and view all the answers

Which of the following best describes the function of the S layer in bacteria?

<p>It provides stability, attachment, and drug resistance. (D)</p> Signup and view all the answers

What is a typical characteristic of bacterial capsules and slime layers?

<p>They are usually composed of polysaccharides and correlated with virulence. (B)</p> Signup and view all the answers

What is a key feature of acid-fast bacteria that makes them difficult to detect and treat?

<p>The presence of mycolic acid (waxy lipid) in their cell walls. (C)</p> Signup and view all the answers

Which characteristic is unique to Mycoplasma species?

<p>They have sterols in their membrane and lack a cell wall. (D)</p> Signup and view all the answers

Where is bacterial DNA typically stored?

<p>In the nucleoid. (C)</p> Signup and view all the answers

What is the function of plasmids in bacteria?

<p>To carry extra genes for metabolism or pathogenicity. (D)</p> Signup and view all the answers

What is the main function of bacterial ribosomes?

<p>To synthesize proteins. (B)</p> Signup and view all the answers

What is the primary function of peptidoglycan in bacteria?

<p>To protect the cell from osmotic stress and maintain its shape. (D)</p> Signup and view all the answers

Which of the following components are the 'bricks' that form the glycan chains in peptidoglycan?

<p>N-acetylglucosamine (NAG) and N-acetylmuramic acid (NAM) (B)</p> Signup and view all the answers

A bacterium is placed in a hypotonic solution. What is the likely outcome?

<p>Water will move into the cell. (B)</p> Signup and view all the answers

Which of the following is a component of Gram-negative bacteria, but not Gram-positive bacteria?

<p>An outer membrane containing lipopolysaccharide (LPS). (A)</p> Signup and view all the answers

What is the function of the lipid A portion of lipopolysaccharide (LPS)?

<p>To act as an endotoxin, causing a toxic response in the host (C)</p> Signup and view all the answers

Which of the following describes the structure of the peptidoglycan layer in Gram-positive bacteria?

<p>A 20-80nm thick layer, with embedded teichoic and lipoteichoic acids. (D)</p> Signup and view all the answers

What is the role of the peptide crosslinks in peptidoglycan?

<p>To tie the glycan chains together. (C)</p> Signup and view all the answers

What is the effect of lysozyme on bacteria?

<p>It breaks the glycan chains, leading to lysis. (B)</p> Signup and view all the answers

Flashcards

Cell membrane (Prokaryotes)

The outer boundary of a prokaryotic cell, composed of a phospholipid bilayer with embedded proteins. It acts as a selective barrier, controlling what enters and exits the cell.

Osmosis

The process by which water moves across a semi-permeable membrane from an area of high water concentration to an area of low water concentration.

Proton Motive Force (PMF)

A potential energy source for prokaryotic cells. It arises from a difference in concentration of hydrogen ions (protons) across the cell membrane. This gradient is used to drive ATP production and other metabolic processes.

Passive Diffusion

Movement of molecules across a cell membrane without requiring energy. This happens when molecules move from an area of high concentration to an area of low concentration.

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Facilitated Diffusion & Active Transport

Movement of molecules across a cell membrane that requires the help of transport proteins. These proteins facilitate the movement of molecules across the membrane, either down or against their concentration gradient.

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Hypotonic

A solution with a lower solute concentration compared to the cell. Water moves into the cell.

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Hypertonic

A solution with a higher solute concentration compared to the cell. Water moves out of the cell.

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Isotonic

A solution with the same solute concentration as the cell. No net water movement occurs.

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Bacterial cell wall

A rigid structure outside the bacterial cell membrane that provides shape and protects against osmotic pressure. Comprised of peptidoglycan.

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Peptidoglycan

The main structural component of bacterial cell walls. Provides rigidity, helps resist osmotic stress, and gives shape.

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Gram stain

A differential staining process that classifies bacteria into Gram-positive and Gram-negative based on cell wall structure.

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Gram-positive bacteria

Bacteria with a thick layer of peptidoglycan that retains the crystal violet stain. Usually susceptible to penicillin.

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Gram-negative bacteria

Bacteria with a thinner layer of peptidoglycan and an outer membrane, which prevents the crystal violet stain from being retained. Usually resistant to penicillin.

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What is coli O157:H7?

A type of lipopolysaccharide (LPS) found in E. coli bacteria, known for causing severe food poisoning and diarrheal illness.

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What is lipopolysaccharide (LPS)?

A complex molecule found in the outer membrane of Gram-negative bacteria. It triggers a strong immune response in humans, leading to various inflammatory and toxic effects.

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What is septic shock?

Severe, life-threatening condition caused by an overwhelming immune response, often triggered by bacterial infections. It leads to dangerously low blood pressure and widespread inflammation throughout the body.

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What is an S layer?

A protein layer found on the surface of some bacteria. It provides structural integrity, offers protection against environmental challenges, and helps bacteria resist antibiotics.

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What are bacterial polysaccharide sheaths?

Capsules, slime layers, and films are protective layers outside the bacterial cell wall, primarily composed of polysaccharides. They help bacteria evade the immune system, adhere to surfaces, and facilitate communication.

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What are acid-fast bacteria?

Bacteria that possess a unique cell wall structure with a high content of mycolic acid, a waxy lipid. This makes them resistant to many antibiotics and staining techniques.

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What are mycoplasmas?

Bacteria that lack a peptidoglycan cell wall and instead rely on a membrane containing sterols for structural support. They are typically small and pleomorphic, meaning they vary in shape.

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What is the nucleoid?

The region within a bacterial cell where the DNA is located, but not enclosed by a membrane like in eukaryotic cells.

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Bacterial Inclusions

Storage structures within bacteria that accumulate various materials such as energy substrates, metabolic precursors, and even orientation markers. These inclusions can be characteristic of specific bacterial species, aiding in identification.

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Pili/Fimbriae

Microscopic filament-like structures extending from the bacterial cell surface, often involved in attaching to surfaces, a crucial step in colonization and disease development. Certain pili (sex pili) play a role in transferring genetic information between bacteria.

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Bacterial Endospores

Resistant, dormant forms of certain bacteria, capable of surviving harsh environmental conditions. They are formed inside bacterial cells and are highly resistant to disinfection methods. Endospore formation poses a significant medical challenge, as these structures can persist even in the human body.

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Endospore Formation

A survival mechanism employed by some bacteria, involving encapsulation within a protective, dormant form. These spores are highly resistant to heat, radiation, and chemicals.

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Bacterial Flagella

Long whip-like appendages that propel bacteria through their surroundings. Flagellar rotation is driven by a proton motive force (PMF), a source of potential energy in bacteria.

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Cyanobacteria

A group of photosynthetic bacteria that utilize chlorophyll a and produce oxygen. These bacteria are often found in aquatic environments and possess thylakoids, similar to those found in plant cells.

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Green and Purple Sulfur Bacteria

A diverse group of photosynthetic bacteria that rely on bacteriochlorophyll for energy capture. They typically inhabit anaerobic environments, those lacking oxygen, and utilize sulfur in their energy processes.

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Archaea

A unique domain of life, separate from both bacteria and eukaryotes, with distinct evolutionary origins. Archaea are often found in extreme environments and possess unique adaptations that distinguish them from other living organisms.

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Study Notes

Prokaryotic Structure

  • Prokaryotes lack a nucleus, storing DNA in a nucleoid, often as a single circular chromosome.
  • Plasmids, extrachromosomal DNA, may be present, carrying genes for metabolism or pathogenicity. They can be transferred between species.
  • Bacterial genomes typically range from 3 to 5 million base pairs in size.
  • Prokaryotes have ribosomes, involved in protein synthesis, that can be free in the cytoplasm; sometimes associating with mRNA, forming polysomes.

Prokaryotic Cell Wall

  • Bacteria have cell walls, primarily composed of peptidoglycan.
  • Peptidoglycan is a rigid structure, offering protection from osmotic stress.
  • Its composition differs between Gram-positive and Gram-negative bacteria.
  • Gram-positive walls are thick and retain crystal violet during Gram staining.
  • Gram-negative walls are thinner, appearing reddish-pink after Gram staining.
  • Gram-positive cells lack an outer membrane, unlike gram-negative cells.
  • In Gram-negative bacteria, an outer membrane containing lipopolysaccharide (LPS) provides protection.
  • LPS is toxic when released and can trigger septic shock.
  • Teichoic acids are embedded in Gram-positive walls, contributing to rigidity.

Additional Layers

  • Some bacteria have additional layers like capsules, slime layers, and s-layers that enhances stability, aid attachment, and resist drugs.
  • Capsules are polysaccharide layers, promoting attachment, reducing phagocytosis, and assisting in colony formation.
  • Slime layers are also polysaccharide, similarly promoting attachment, pathogenicity, and colony formation.
  • S-layers are protein layers, vital to stability, attachment, and drug resistance, for prokaryotes.

Bacterial Storage Inclusions

  • Prokaryotes contain inclusions, vacuoles that store various materials including energy substrates, metabolic precursors, sulfur crystals, and polyphosphate crystals.
  • Inclusions may also aid bacterial orientation, providing species-specific characteristics.

Bacterial Morphology

  • Bacteria exhibit various shapes and arrangements, including cocci (spherical), bacilli (rod-shaped), coccobacilli (short rods), vibrios (curved rods), spirilla (spiral shapes), and spirochetes (flexible spirals).

Bacterial Endospores

  • Endospores are metabolically inactive, dormant structures, that enhance long-term survival.
  • Endospores typically form within a bacterial cell and are extremely resistant to harsh conditions, disinfectants, and some sterilization procedures.

Bacterial Attachment & Movement

  • Pili (or fimbriae) are structures that help bacteria attach to surfaces, enhancing colonization and aiding in pathogenicity.
  • Sex pili are specific types of pili that facilitate genetic transfer between bacteria during conjugation.
  • Flagella enable bacterial motility, using energy generated by the proton motive force (PMF). Bacteria can move towards or away from attractants or repellents.

Photosynthetic Bacteria

  • Cyanobacteria (blue-green algae) are photosynthetic bacteria that produce oxygen.
  • They use chlorophyll a for photosynthesis, replicating eukaryotic thylakoids.
  • Green and purple sulfur bacteria perform photosynthesis in anaerobic environments and use sulfur compounds like H2S as energy sources.

Archaea

  • Archaea form a distinct domain of life differing from Bacteria and Eukarya with unique features including their cell walls not containing peptidoglycan.
  • Their cell membranes are constructed from ether-linked lipids instead of ester-linked lipids found in Bacteria and Eukarya.
  • Archaea demonstrate a wide range of metabolic adaptations allowing these prokaryotic microorganisms to survive in extreme environments like hot springs, hypersaline areas, and anaerobic conditions.

Bacterial Genome (DNA)

  • Bacteria store their DNA within the nucleoid region without a membrane-bound nucleus.
  • The DNA is typically organized as a single, circular chromosome.

Bacterial Ribosomes (Protein Synthesis)

  • Bacterial ribosomes are protein and RNA complexes responsible for protein synthesis.
  • Notably, ribosome size (70S) is distinct from eukaryotic ribosomes (80S).

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