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Questions and Answers
What is the function of the DnaA protein in DNA replication in prokaryotes?
What is the function of the DnaA protein in DNA replication in prokaryotes?
- It breaks the hydrogen bonds between base pairs to initiate replication
- It creates the Y-shaped replication fork structure
- It binds to the G/C-rich sequences in the ori site
- It binds to the A/T-rich sequences in the ori site and stretches the double-stranded DNA (correct)
Why is the ori site in prokaryotes mostly comprised of A/T-rich sequences?
Why is the ori site in prokaryotes mostly comprised of A/T-rich sequences?
- A/T-rich sequences are stronger and more stable than G/C-rich sequences
- A/T-rich sequences are more compatible with the DnaA protein
- G/C-rich sequences are not present in prokaryotic DNA
- A/T-rich sequences are easier to break than G/C-rich sequences (correct)
How many replication forks are created during DNA replication in prokaryotes?
How many replication forks are created during DNA replication in prokaryotes?
- Two (correct)
- Four
- One
- Three
Why is the ori site in prokaryotes mostly composed of A/T-rich sequences?
Why is the ori site in prokaryotes mostly composed of A/T-rich sequences?
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How many replication forks are created during DNA replication in prokaryotes?
How many replication forks are created during DNA replication in prokaryotes?
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Study Notes
DNA Replication in Prokaryotes
- DnaA protein binds to the origin of replication (ori) and unwinds the double helix, facilitating the recruitment of other proteins necessary for DNA replication.
- The ori site in prokaryotes is mostly comprised of A/T-rich sequences because they are more easily melted, allowing for the initiation of DNA replication.
- During DNA replication in prokaryotes, two replication forks are created, one moving clockwise and the other counterclockwise, allowing for bidirectional replication.
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