Prokaryotes - Monera - Biodiversity

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Questions and Answers

What is the primary way pathogenic bacteria cause disease?

  • By multiplying uncontrollably in tissues
  • By altering the host's immune system
  • By releasing exotoxins or endotoxins (correct)
  • By invading host cells directly

What is the role of horizontal gene transfer in pathogenic bacteria?

  • It improves nutrient absorption in bacteria
  • It increases the speed of bacterial reproduction
  • It enhances bacterial resistance to antibiotics
  • It spreads genes associated with virulence (correct)

Which bacterium is commonly used in gene cloning?

  • Thermus aquaticus
  • Agrobacterium tumefaciens
  • Escherichia coli (correct)
  • Staphylococcus aureus

What is bioremediation primarily used for?

<p>To remove pollutants from the environment (B)</p> Signup and view all the answers

Which statement about endotoxins is true?

<p>They are released only when bacteria die (B)</p> Signup and view all the answers

What distinguishes prokaryotes from eukaryotes?

<p>Prokaryotes lack a nucleus. (D)</p> Signup and view all the answers

Which of the following best describes the size of most prokaryotic cells?

<p>They have diameters of 0.5 – 5 µm. (D)</p> Signup and view all the answers

In what environments can prokaryotes thrive?

<p>In extreme conditions including acidic, salty, or hot environments. (C)</p> Signup and view all the answers

What is the primary function of a capsule in prokaryotes?

<p>To protect against dehydration and immune attacks (D)</p> Signup and view all the answers

What are the two primary lineages of prokaryotes?

<p>Bacteria and Archaea. (C)</p> Signup and view all the answers

Which of the following is NOT a common shape of prokaryotes?

<p>Square (squarili). (D)</p> Signup and view all the answers

Which structures are primarily responsible for the motility in prokaryotes?

<p>Flagella (B)</p> Signup and view all the answers

What type of DNA structure do prokaryotes possess?

<p>Circular with fewer proteins (A)</p> Signup and view all the answers

What is the primary method of reproduction for prokaryotes?

<p>Binary fission (D)</p> Signup and view all the answers

How do fimbriae assist prokaryotes?

<p>They allow adherence to surfaces or to each other (A)</p> Signup and view all the answers

Which process involves a prokaryotic cell taking up foreign DNA from its environment?

<p>Transformation (A)</p> Signup and view all the answers

What term describes the movement of genes between bacteria through viruses?

<p>Transduction (B)</p> Signup and view all the answers

What is a characteristic of endospores in prokaryotes?

<p>They are resistant structures developed under nutrient deficiency (B)</p> Signup and view all the answers

What is one of the main contributors to genetic diversity in prokaryotes?

<p>Horizontal gene transfer (C)</p> Signup and view all the answers

What type of movement does positive taxis refer to in prokaryotes?

<p>Movement towards a favorable stimulus (A)</p> Signup and view all the answers

What defines the presence of a nucleus in eukaryotic cells compared to prokaryotic cells?

<p>Eukaryotic cells have a defined nucleus containing their DNA (A)</p> Signup and view all the answers

Which of the following accurately describes prokaryotic generation times?

<p>Short, often ranging from 20 minutes to a few hours (C)</p> Signup and view all the answers

Which of the following organisms is likely to be antibiotic resistant?

<p>Gram-negative bacteria (C)</p> Signup and view all the answers

In prokaryotes, what is the effect of rapid reproduction on mutations?

<p>It causes mutations to accumulate quickly over time (A)</p> Signup and view all the answers

Which of these accurately describes the process of conjugation in prokaryotes?

<p>Sharing of genetic material directly through cell contacts (A)</p> Signup and view all the answers

What is the primary reason prokaryotes evolve rapidly?

<p>Frequent genetic recombination and rapid reproduction (C)</p> Signup and view all the answers

What is a common pathogenic parasite mentioned in the content?

<p>Treponema pallidum (B)</p> Signup and view all the answers

What role do chemoheterotrophic prokaryotes primarily serve in ecosystems?

<p>Decomposers (D)</p> Signup and view all the answers

Which type of ecological interaction is characterized by one organism benefiting while the other is neither helped nor harmed?

<p>Commensalism (D)</p> Signup and view all the answers

Which group of bacteria is known for their role in antibiotic production?

<p>Actinomycetes (D)</p> Signup and view all the answers

What significant impact do prokaryotes have on plant growth?

<p>They increase the availability of essential nutrients. (D)</p> Signup and view all the answers

Which characteristic distinguishes cyanobacteria from other bacteria?

<p>Ability to perform oxygen-generating photosynthesis (D)</p> Signup and view all the answers

What is one way that mutualistic bacteria in human guts benefit their host?

<p>By digesting food that the host cannot break down (D)</p> Signup and view all the answers

Which of the following correctly describes proteobacteria?

<p>Diverse group of gram-negative bacteria with various lifestyles (D)</p> Signup and view all the answers

What is the primary function of a sex pilus in bacteria?

<p>To transfer genetic material (B)</p> Signup and view all the answers

Which type of prokaryote is poisoned by oxygen and relies on fermentation?

<p>Obligate anaerobes (C)</p> Signup and view all the answers

What significant classification change occurred due to molecular systematics?

<p>Prokaryotes were split into bacteria and archaea (C)</p> Signup and view all the answers

Which of the following statements is true about methanogens?

<p>They produce methane as a waste product (A)</p> Signup and view all the answers

What characteristic is NOT associated with chlamydias?

<p>They have a gram-positive cell wall (A)</p> Signup and view all the answers

What environment do extremophiles most likely inhabit?

<p>Highly saline or extremely hot environments (B)</p> Signup and view all the answers

Which prokaryotic group includes organisms that predominantly rely on fermentation?

<p>Methanogens (C)</p> Signup and view all the answers

What is the role of facultative anaerobes in relation to oxygen?

<p>They can survive with or without oxygen (B)</p> Signup and view all the answers

Flashcards

Prokaryotes

Microscopic organisms lacking a nucleus, most are single-celled.

Bacteria & Archaea

Two lineages that are prokaryotic and make up Kingdom Monera.

Bacterial Shapes

Cocci (spherical), Bacilli (rod-shaped) are common shapes among bacteria.

Prokaryotic Size

Typically smaller (0.5-5 µm) than eukaryotic cells (10-100 µm).

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Prokaryotic Diversity

Prokaryotes thrive in a wide variety of environments.

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Capsule vs. Slime Layer

Both are sticky outer layers on prokaryotes, but capsules are denser and well-defined, while slime layers are less organized.

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Prokaryotic Adaptation: Capsule & Slime Layer

Capsules and slime layers help prokaryotes adhere to surfaces, resist drying out, and even evade host immune systems.

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What are Endospores?

Tough, multi-layered structures formed by some prokaryotes when they lack essential nutrients, helping them survive harsh conditions.

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Fimbriae vs. Pili

Both are hair-like appendages, but fimbriae help prokaryotes attach to surfaces, while pili help them pull two cells together for DNA transfer.

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What is Taxis?

Directed movement of prokaryotes towards or away from a stimulus.

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Flagella in Prokaryotes

Flagella are whip-like structures that propel prokaryotes. They have a motor, hook, and filament.

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Prokaryotes vs. Eukaryotes: Compartmentalization

Prokaryotes lack complex compartmentalization compared to eukaryotes, although they may have specialized membranes for metabolic functions.

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Prokaryotes vs. Eukaryotes: DNA

Prokaryotes have circular chromosomes with fewer proteins compared to the linear chromosomes with more proteins in eukaryotes.

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Internal Organisation of Prokaryotes

Prokaryotes have specialized membranes, often infoldings of the plasma membrane, to perform metabolic functions. Examples include respiratory membranes and thylakoid membranes in photosynthetic prokaryotes.

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Prokaryotic Reproduction

Prokaryotes reproduce asexually through binary fission, where one cell divides into two identical daughter cells. This process is rapid, allowing for rapid population growth in favorable environments.

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Key Features of Prokaryotic Reproduction

Prokaryotes reproduce quickly through binary fission, have short generation times, and evolve rapidly due to their small size and efficient reproduction.

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Genetic Diversity in Prokaryotes

Prokaryotes exhibit high genetic diversity due to rapid reproduction, mutations, and genetic recombination.

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Mutations in Prokaryotes

While mutation rates during binary fission are low, the rapid rate of reproduction allows mutations to accumulate quickly in a population.

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Genetic Recombination

The combining of DNA from two sources, called genetic recombination, contributes to diversity in prokaryotes. This occurs through transformation, transduction, and conjugation.

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Horizontal Gene Transfer

The movement of genes among individuals from different species, called horizontal gene transfer, further increases prokaryotic diversity.

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Transformation

Transformation is the process where a prokaryotic cell takes up and incorporates foreign DNA from the surrounding environment.

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What is conjugation?

The process of transferring genetic material between prokaryotic cells that are temporarily joined.

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What is the role of a sex pilus in conjugation?

A sex pilus is a structure that connects two prokaryotic cells, allowing for the transfer of genetic material during conjugation.

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What is an obligate aerobe?

A prokaryote that absolutely requires oxygen for cellular respiration.

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What is an obligate anaerobe?

A prokaryote that cannot tolerate oxygen and uses fermentation or anaerobic respiration for energy.

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What is a facultative anaerobe?

A prokaryote that can survive with or without oxygen.

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What is molecular systematics?

Using genetic analysis to investigate the evolutionary relationships between organisms.

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Extreme Halophiles

Archaea that thrive in extremely salty environments.

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Extreme Thermophiles

Archaea that thrive in very hot environments.

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Pathogenic Prokaryotes

Prokaryotes that cause diseases in humans.

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Exotoxins

Toxic proteins secreted by some bacteria that cause disease even when the bacteria are not present.

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Endotoxins

Toxic components of bacterial cell walls that are released only when the bacteria die.

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Bioremediation

Using organisms, like bacteria, to clean up pollutants in the environment.

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Transgenic Plants

Plants genetically modified to carry genes from other organisms, often using bacteria.

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Spirochetes

A group of spiral-shaped bacteria, many live freely, but some are known for causing serious diseases.

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Cyanobacteria

Photosynthetic bacteria, essential for generating oxygen, can form chains or be single-celled.

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Gram-positive bacteria

Bacteria with a thick peptidoglycan layer in their cell wall, many are decomposers or produce antibiotics.

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Proteobacteria

A diverse and large group of bacteria, they can use various energy sources and are found in many environments.

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Prokaryotic Roles: Chemical Recycling

Prokaryotes play a vital role in breaking down dead organisms and waste products, allowing nutrients to be reused.

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Prokaryotic Symbiosis: Mutualism

A relationship where both organisms involved benefit from each other.

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Prokaryotic Symbiosis: Parasitism

A relationship where one organism benefits at the expense of another, often causing harm.

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Mutualistic Bacteria in Humans

Beneficial bacteria in our gut help digest food that our body can't, playing a crucial role in our health.

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Study Notes

Prokaryotes - Monera - Biodiversity

  • Prokaryotes are the first organisms on Earth
  • They lack a nucleus and are mostly unicellular
  • Most prokaryotic cells are 0.5-5μm in diameter, much smaller than eukaryotic cells (10-100μm) — exception: Thiomargarita namibiensis (750μm)
  • Prokaryotes are smaller and simpler than eukaryotes, but make up for it in numbers
  • They thrive in almost all environments, including extreme conditions (acidic, salty, cold, hot) that are unsuitable for most other organisms
  • Divided into two lineages: Bacteria and Archaea
  • Both are prokaryotes, belonging to Kingdom Monera

Features of Prokaryotes

  • Shape:

    • Spherical (cocci): single, pairs, chains, clusters
    • Rod-shaped (bacilli): single, chains
    • Spiral: comma-like, loose coils, corkscrew-shaped -Exceptions: Flat and square-shaped Haloquadratum walsbyi
  • Cell-surface structure:

    • Cell wall maintains cell shape, protects from bursting in hypotonic environments for nearly all prokaryotes
    • Eukaryotic cell walls are made of cellulose or chitin
    • Bacterial cell walls contain peptidoglycan, a network of sugar polymers cross-linked by polypeptides
    • Archaeal cell walls contain a variety of polysaccharides and proteins but lack peptidoglycan
    • Gram-staining differentiates bacteria based on the amount of peptidoglycan in the cell wall: -Gram-positive bacteria have a thick peptidoglycan layer and stain purple -Gram-negative bacteria have a thin peptidoglycan layer and an outer membrane and stain red
    • Capsule: sticky layer of polysaccharide or protein around the cell wall for protection and adherence
    • Slime layer: a less organized, sticky layer, which also helps with adherence.
    • Endospores: tough, multi-layered resistant cells formed by some when nutrients are scarce
  • Motility:

    • Some prokaryotes use flagella for movement
    • Flagella are motor, hook, and filament parts
  • Internal organization and DNA:

    • Simpler than those of eukaryotes
    • No membrane-enclosed organelles
    • Less DNA (circular chromosome with few proteins)
    • Often possess plasmids (small rings of DNA)
    • DNA lies in a nucleoid area within the cell.
    • Specialised metabolic membranes (infoldings of the plasma membrane) for different functions
  • Reproduction:

    • Binary fission: a single cell divides into two identical cells
    • Rapid reproduction rates allow for quick adaptations
  • Mode of Nutrition:

    • Autotrophs make their own food: -Photoautotrophs use light -Chemoautotrophs use inorganic molecules,
    • Heterotrophs consume organic compounds as food: -Photoheterotrophs use light -Chemoheterotrophs consume organic molecules.

Factors Influencing Genetic Diversity

  • Prokaryotes show substantial genetic variation
  • Main factors:
    • Rapid reproduction
    • Mutation
    • Genetic recombination

Genetic Recombination

  • Combining DNA from different sources
  • Key methods include: -Transformation -Transduction -Conjugation

Roles of Prokaryotes in the Biosphere

  • Chemical Recycling: -Decomposers break down dead organisms and waste products
    • Increase the availability of nitrogen, phosphorus, and potassium for plant growth
  • Ecological Interactions: -Symbiosis (live in close contact) -Mutualistic: both benefit -Commensalism: one benefits, the other is unaffected -Parasitic: one benefits, the other is harmed, but not killed; Pathogens are parasitic microorganisms that cause disease
  • Impact on humans: -Mutualistic: live in the human gut, aid in digestion -Pathogenic: cause disease; many diseases are caused by prokaryotes; Toxins cause disease (exotoxins released by the organism even if it is dead, endotoxins released by cell lysis)

Prokaryotes in Research & Technology

  • Used for DNA technology like gene cloning
  • Produce enzymes like Taq DNA polymerase for PCR
  • Bioremediation: clearing pollutants
  • Produce vitamins, antibiotics, and hormones
  • Produce bio-plastics

Archaea

  • Some live in extreme environments
  • Include extreme halophiles (salt-loving), extreme thermophiles (heat-loving), and methanogens (methane-producing organisms)

Bacteria

  • Wide diversity, including: -Chlamydias (live within animal cells) -Spirochetes (spiral-shaped bacteria) -Cyanobacteria (photosynthetic bacteria) -Gram-positive bacteria (thick peptidoglycan layer) -Proteobacteria (diverse, includes aerobes and anaerobes)

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