Probability: Definitions and Rules

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Questions and Answers

The image of a cell is 5 cm wide, and its actual size is 0.02 mm. What is the magnification?

2500

A cell has a magnification of 500x. If the image size is 2 mm, calculate the actual size of the cell in micrometers.

4 μm

If a plant cell's actual length is 0.05 mm, and it appears 5 mm long under a microscope, what is the magnification?

100x

How does increased magnification affect the field of view when observing a specimen under a microscope?

<p>Decreases the field of view.</p> Signup and view all the answers

What is the primary limitation of using light microscopy to view extremely small cellular structures?

<p>Limited resolution.</p> Signup and view all the answers

Why do electron microscopes have a higher resolution compared to light microscopes?

<p>Electron microscopes use electrons which have a much smaller wavelength than light.</p> Signup and view all the answers

Name two subcellular structures that became more clearly understood due to the advent of electron microscopy?

<p>Ribosomes, Mitochondria</p> Signup and view all the answers

If a light microscope has a magnification of 400x and an electron microscope has a magnification of 100,000x, how many times greater is the electron microscope's magnification?

<p>250 times greater</p> Signup and view all the answers

A student measures a cell image to be 20 mm under a microscope with a magnification of 500x. What is the actual size of the cell in micrometers?

<p>40 μm</p> Signup and view all the answers

What type of microscope, electron or light, would be more effective in observing the detailed structure of a virus?

<p>Electron microscope</p> Signup and view all the answers

Explain how the use of staining techniques enhances the observation of cells under a light microscope.

<p>Staining increases contrast, making cellular structures more visible.</p> Signup and view all the answers

A light microscope has an objective lens with a magnification of 40x and an eyepiece lens with a magnification of 10x. What is the total magnification?

<p>400x</p> Signup and view all the answers

Describe one advantage of using a light microscope over an electron microscope for observing living cells.

<p>Light microscopy allows for observation of living cells, while electron microscopy requires fixed/dead samples.</p> Signup and view all the answers

How does the specimen preparation differ between light and electron microscopy, particularly regarding the thickness of the sample?

<p>Electron microscopy requires much thinner samples than light microscopy.</p> Signup and view all the answers

Explain why electron microscopy is usually performed under a vacuum.

<p>To prevent electrons from colliding with air molecules.</p> Signup and view all the answers

What is the function of the condenser lens in a light microscope?

<p>The condenser lens focuses light onto the specimen.</p> Signup and view all the answers

If a cell's diameter is measured as 50 micrometers under a microscope, what is its diameter in millimeters?

<p>0.05 mm</p> Signup and view all the answers

Describe how the development of electron microscopy contributed to a better understanding of viruses.

<p>Electron microscopy allowed for the visualization of the structure and components of viruses.</p> Signup and view all the answers

A student views a specimen under a microscope using a 10x objective lens. They then switch to a 40x objective lens. How does this affect the amount of detail they can observe?

<p>The detail observed will increase.</p> Signup and view all the answers

Explain how the use of fluorescent dyes in light microscopy has enhanced the ability to study specific cellular components.

<p>Fluorescent dyes bind to specific structures, allowing them to be visualized with greater precision.</p> Signup and view all the answers

Flashcards

What is magnification?

The ratio of the image size to the real size of an object.

How to calculate magnification

To calculate magnification, divide the size of the image by the size of the real object.

Electron vs. Light Microscopes

Electron microscopes have higher resolution and magnification compared to light microscopes.

What electron microscopy revealed

Electron microscopy allows us to see ribosomes and mitochondria

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Study Notes

  • Includes definitions, methods of assigning probability, axioms, and basic probability rules.

Basic Definitions

  • An experiment is a process that produces one or more outcomes.
  • The sample space, denoted by ( S ), is the set of all possible outcomes of an experiment.
  • An event, denoted by ( E ), is a subset of the sample space.
  • An elementary event consists of only one outcome.
  • Mutually exclusive events are events with no outcomes in common (disjoint).

Methods of Assigning Probability

  • Classical Approach (Equally Likely Outcomes)
    • If an experiment has $n$ possible outcomes, the probability of each outcome is $\frac{1}{n}$.
    • The probability of event ( E ) is: ( P(E) = \frac{\text{Number of outcomes in } E}{\text{Total number of outcomes in } S} = \frac{n(E)}{n(S)} )
  • Relative Frequency Approach
    • The probability of an event is approximated by the relative frequency of its occurrence in a large number of trials.
    • ( P(E) \approx \frac{\text{Number of times } E \text{ occurs}}{\text{Total number of trials}} )
  • Subjective Approach
    • Probability is based on personal judgment or belief.

Axioms of Probability

  • Axioms define the fundamental rules for probability:
    • For any event ( E ), ( 0 \leq P(E) \leq 1 ).
    • The probability of the sample space ( S ) is ( P(S) = 1 ).
    • For mutually exclusive events ( E_1, E_2, E_3, \dots ): ( P(E_1 \cup E_2 \cup E_3 \cup \dots) = P(E_1) + P(E_2) + P(E_3) + \dots )

Basic Probability Rules

  • Complement Rule
    • For any event ( E ), ( P(E^c) = 1 - P(E) ), where ( E^c ) is the complement of ( E ).
  • Addition Rule
    • For any two events ( A ) and ( B ): ( P(A \cup B) = P(A) + P(B) - P(A \cap B) )
  • If ( A ) and ( B ) are mutually exclusive: ( P(A \cup B) = P(A) + P(B) ) since ( P(A \cap B) = 0 ).
  • Conditional Probability
    • The probability of event ( A ) given that event ( B ) has occurred is: ( P(A|B) = \frac{P(A \cap B)}{P(B)} ), where ( P(B) > 0 ).
  • Multiplication Rule
    • Joint probability can be calculated as: ( P(A \cap B) = P(A|B) \cdot P(B) = P(B|A) \cdot P(A) )
  • Independent Events
    • Two events ( A ) and ( B ) are independent if the occurrence of one does not affect the probability of the other.
      • ( P(A|B) = P(A) ), ( P(B|A) = P(B) ), or ( P(A \cap B) = P(A) \cdot P(B) )
  • Total Probability Rule
    • For mutually exclusive and exhaustive events ( B_1, B_2, \dots, B_n ), ( P(A) = \sum_{i=1}^{n} P(A|B_i) \cdot P(B_i) ) for any event ( A ).
  • Bayes' Theorem
    • ( P(B_i|A) = \frac{P(A|B_i) \cdot P(B_i)}{\sum_{j=1}^{n} P(A|B_j) \cdot P(B_j)} ) for n mutually exclusive and exhaustive events ( B_1, B_2, \dots, B_n ).

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