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Questions and Answers
The image of a cell is 5 cm wide, and its actual size is 0.02 mm. What is the magnification?
The image of a cell is 5 cm wide, and its actual size is 0.02 mm. What is the magnification?
2500
A cell has a magnification of 500x. If the image size is 2 mm, calculate the actual size of the cell in micrometers.
A cell has a magnification of 500x. If the image size is 2 mm, calculate the actual size of the cell in micrometers.
4 μm
If a plant cell's actual length is 0.05 mm, and it appears 5 mm long under a microscope, what is the magnification?
If a plant cell's actual length is 0.05 mm, and it appears 5 mm long under a microscope, what is the magnification?
100x
How does increased magnification affect the field of view when observing a specimen under a microscope?
How does increased magnification affect the field of view when observing a specimen under a microscope?
What is the primary limitation of using light microscopy to view extremely small cellular structures?
What is the primary limitation of using light microscopy to view extremely small cellular structures?
Why do electron microscopes have a higher resolution compared to light microscopes?
Why do electron microscopes have a higher resolution compared to light microscopes?
Name two subcellular structures that became more clearly understood due to the advent of electron microscopy?
Name two subcellular structures that became more clearly understood due to the advent of electron microscopy?
If a light microscope has a magnification of 400x and an electron microscope has a magnification of 100,000x, how many times greater is the electron microscope's magnification?
If a light microscope has a magnification of 400x and an electron microscope has a magnification of 100,000x, how many times greater is the electron microscope's magnification?
A student measures a cell image to be 20 mm under a microscope with a magnification of 500x. What is the actual size of the cell in micrometers?
A student measures a cell image to be 20 mm under a microscope with a magnification of 500x. What is the actual size of the cell in micrometers?
What type of microscope, electron or light, would be more effective in observing the detailed structure of a virus?
What type of microscope, electron or light, would be more effective in observing the detailed structure of a virus?
Explain how the use of staining techniques enhances the observation of cells under a light microscope.
Explain how the use of staining techniques enhances the observation of cells under a light microscope.
A light microscope has an objective lens with a magnification of 40x and an eyepiece lens with a magnification of 10x. What is the total magnification?
A light microscope has an objective lens with a magnification of 40x and an eyepiece lens with a magnification of 10x. What is the total magnification?
Describe one advantage of using a light microscope over an electron microscope for observing living cells.
Describe one advantage of using a light microscope over an electron microscope for observing living cells.
How does the specimen preparation differ between light and electron microscopy, particularly regarding the thickness of the sample?
How does the specimen preparation differ between light and electron microscopy, particularly regarding the thickness of the sample?
Explain why electron microscopy is usually performed under a vacuum.
Explain why electron microscopy is usually performed under a vacuum.
What is the function of the condenser lens in a light microscope?
What is the function of the condenser lens in a light microscope?
If a cell's diameter is measured as 50 micrometers under a microscope, what is its diameter in millimeters?
If a cell's diameter is measured as 50 micrometers under a microscope, what is its diameter in millimeters?
Describe how the development of electron microscopy contributed to a better understanding of viruses.
Describe how the development of electron microscopy contributed to a better understanding of viruses.
A student views a specimen under a microscope using a 10x objective lens. They then switch to a 40x objective lens. How does this affect the amount of detail they can observe?
A student views a specimen under a microscope using a 10x objective lens. They then switch to a 40x objective lens. How does this affect the amount of detail they can observe?
Explain how the use of fluorescent dyes in light microscopy has enhanced the ability to study specific cellular components.
Explain how the use of fluorescent dyes in light microscopy has enhanced the ability to study specific cellular components.
Flashcards
What is magnification?
What is magnification?
The ratio of the image size to the real size of an object.
How to calculate magnification
How to calculate magnification
To calculate magnification, divide the size of the image by the size of the real object.
Electron vs. Light Microscopes
Electron vs. Light Microscopes
Electron microscopes have higher resolution and magnification compared to light microscopes.
What electron microscopy revealed
What electron microscopy revealed
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Study Notes
- Includes definitions, methods of assigning probability, axioms, and basic probability rules.
Basic Definitions
- An experiment is a process that produces one or more outcomes.
- The sample space, denoted by ( S ), is the set of all possible outcomes of an experiment.
- An event, denoted by ( E ), is a subset of the sample space.
- An elementary event consists of only one outcome.
- Mutually exclusive events are events with no outcomes in common (disjoint).
Methods of Assigning Probability
- Classical Approach (Equally Likely Outcomes)
- If an experiment has $n$ possible outcomes, the probability of each outcome is $\frac{1}{n}$.
- The probability of event ( E ) is: ( P(E) = \frac{\text{Number of outcomes in } E}{\text{Total number of outcomes in } S} = \frac{n(E)}{n(S)} )
- Relative Frequency Approach
- The probability of an event is approximated by the relative frequency of its occurrence in a large number of trials.
- ( P(E) \approx \frac{\text{Number of times } E \text{ occurs}}{\text{Total number of trials}} )
- Subjective Approach
- Probability is based on personal judgment or belief.
Axioms of Probability
- Axioms define the fundamental rules for probability:
- For any event ( E ), ( 0 \leq P(E) \leq 1 ).
- The probability of the sample space ( S ) is ( P(S) = 1 ).
- For mutually exclusive events ( E_1, E_2, E_3, \dots ): ( P(E_1 \cup E_2 \cup E_3 \cup \dots) = P(E_1) + P(E_2) + P(E_3) + \dots )
Basic Probability Rules
- Complement Rule
- For any event ( E ), ( P(E^c) = 1 - P(E) ), where ( E^c ) is the complement of ( E ).
- Addition Rule
- For any two events ( A ) and ( B ): ( P(A \cup B) = P(A) + P(B) - P(A \cap B) )
- If ( A ) and ( B ) are mutually exclusive: ( P(A \cup B) = P(A) + P(B) ) since ( P(A \cap B) = 0 ).
- Conditional Probability
- The probability of event ( A ) given that event ( B ) has occurred is: ( P(A|B) = \frac{P(A \cap B)}{P(B)} ), where ( P(B) > 0 ).
- Multiplication Rule
- Joint probability can be calculated as: ( P(A \cap B) = P(A|B) \cdot P(B) = P(B|A) \cdot P(A) )
- Independent Events
- Two events ( A ) and ( B ) are independent if the occurrence of one does not affect the probability of the other.
- ( P(A|B) = P(A) ), ( P(B|A) = P(B) ), or ( P(A \cap B) = P(A) \cdot P(B) )
- Two events ( A ) and ( B ) are independent if the occurrence of one does not affect the probability of the other.
- Total Probability Rule
- For mutually exclusive and exhaustive events ( B_1, B_2, \dots, B_n ), ( P(A) = \sum_{i=1}^{n} P(A|B_i) \cdot P(B_i) ) for any event ( A ).
- Bayes' Theorem
- ( P(B_i|A) = \frac{P(A|B_i) \cdot P(B_i)}{\sum_{j=1}^{n} P(A|B_j) \cdot P(B_j)} ) for n mutually exclusive and exhaustive events ( B_1, B_2, \dots, B_n ).
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