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Principles of Ion Exchange Chromatography

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Principles of Ion Exchange Chromatography

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Questions and Answers

Which step is not part of the ion exchange chromatography process?

Elution

Equilibration

Column Destruction (correct)

Sample Application

What is the primary function of ion exchange chromatography?

To conduct size exclusion for large biomolecules

To analyze the molecular weight of compounds

To isolate covalent molecules from a mixture

To separate and purify ionic or polar molecules based on their charge (correct)

Which of the following factors does not significantly affect ion exchange in chromatography?

Temperature

Ionic Strength

pH of the mobile phase

Size of the ion exchange column (correct)

What type of ion exchange binds negatively charged ions to positively charged functional groups on the resin?

Anion Exchange Signup and view all the answers

Why do stronger interactions result in slower elution from the ion exchange column?

They form more stable complexes with the resin Signup and view all the answers

What is one application of ion exchange chromatography?

Purification of proteins and nucleic acids Signup and view all the answers

What is a common limitation of ion exchange chromatography?

Dependency on the charge properties of the sample Signup and view all the answers

Which of the following describes the mobile phase in ion exchange chromatography?

A buffer solution carrying sample ions Signup and view all the answers

Study Notes

Ion Exchange Chromatography: Key Concepts

Primary Function: Separating and purifying charged molecules based on their affinity for a stationary phase (resin) with opposite charges.
Step Not Involved: Centrifugation.
Factors Affecting Ion Exchange: pH, ionic strength, temperature, and the concentration of mobile phase.
Type of Ion Exchange: Anion exchange binds negatively charged ions to positively charged functional groups on the resin.
Strong Interactions: Stronger interactions between the analyte and the resin lead to slower elution because the analyte spends more time bound to the resin, delaying its movement through the column.
Applications: Purification of proteins, peptides, and other biomolecules, water softening, and removing impurities from various solutions.
Common Limitations: Limited capacity for large molecules and potential for irreversible binding.
Mobile phase: A liquid solution that carries the analytes through the column, often containing buffers, salts, or other compounds to modulate the separation.

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Description

This quiz explores the foundational concepts of ion exchange chromatography, a pivotal technique in separating and purifying ionic molecules based on charge. Delve into the mechanisms of ion exchange resins, the types of ion exchanges, and the operating principles driving this separation process.