18 Questions
What is the optimal amplicon size for the product size range?
~120 bp
What is the acceptable range for primer size according to the text?
18-28 bp
What is the optimal primer Tm (melting temperature) value mentioned in the text?
60°C
What is the maximum allowable Tm difference between the forward and reverse primers according to the text?
3°C
What is the optimal GC% (guanine-cytosine content) range for primers mentioned in the text?
50-60%
What is the recommended setting for 'Max 3' Self complementarity'?
0
What is the primary purpose of the Primer3 software?
To design primers for PCR amplification
Which database is mentioned in the text for retrieving rat gene sequences?
Rat Genome Database (RGD)
What is the recommended approach for providing the target sequence to Primer3?
Save the sequence as a text file and upload it to Primer3
Which species are supported by the Rat Genome Database (RGD) according to the text?
Rat, Mouse, Human, and Chinchilla
What is the purpose of copying the nucleotide sequence from the Rat Genome Database to the NCBI GenBank?
To obtain additional annotations and features
Which of the following steps is not mentioned in the text for primer design using Primer3?
Adjusting parameters for primer design in the Primer3 interface
What should be avoided in the 3' end of a primer to prevent primer-dimer formation?
Repeated runs of 3 or more Cs or Gs
Why is it mentioned to increase the primer setting by 1 if no primers are returned?
To get primers
Which action should be taken after selecting the primers for testing?
Running BLAST and checking for Query coverage
What could promoting mispriming at C or C-rich sequences potentially result in?
Primer-dimer formation
Why is it important to check the E-value after running BLAST on the primers?
To assess the accuracy of the primer sequence alignment
What should be done if there are no results after setting the value to 1 during primer selection?
Increase the value by an increment of 1
This article discusses primer design using Primer3 software for creating primers in molecular biology experiments. It provides insights into designing efficient primers for experiments like PCR. You can learn about the process and best practices for primer design.
Make Your Own Quizzes and Flashcards
Convert your notes into interactive study material.
Get started for free
