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Questions and Answers
What is the optimal amplicon size for the product size range?
What is the optimal amplicon size for the product size range?
- ~120 bp (correct)
- 70-200 bp, 80-150 bp
- 70-200 bp
- 80-150 bp
What is the acceptable range for primer size according to the text?
What is the acceptable range for primer size according to the text?
- 15-25 bp
- 16-24 bp
- 18-28 bp (correct)
- 20-30 bp
What is the optimal primer Tm (melting temperature) value mentioned in the text?
What is the optimal primer Tm (melting temperature) value mentioned in the text?
- 60°C (correct)
- 63°C
- 59°C
- 57°C
What is the maximum allowable Tm difference between the forward and reverse primers according to the text?
What is the maximum allowable Tm difference between the forward and reverse primers according to the text?
What is the optimal GC% (guanine-cytosine content) range for primers mentioned in the text?
What is the optimal GC% (guanine-cytosine content) range for primers mentioned in the text?
What is the recommended setting for 'Max 3' Self complementarity'?
What is the recommended setting for 'Max 3' Self complementarity'?
What is the primary purpose of the Primer3 software?
What is the primary purpose of the Primer3 software?
Which database is mentioned in the text for retrieving rat gene sequences?
Which database is mentioned in the text for retrieving rat gene sequences?
What is the recommended approach for providing the target sequence to Primer3?
What is the recommended approach for providing the target sequence to Primer3?
Which species are supported by the Rat Genome Database (RGD) according to the text?
Which species are supported by the Rat Genome Database (RGD) according to the text?
What is the purpose of copying the nucleotide sequence from the Rat Genome Database to the NCBI GenBank?
What is the purpose of copying the nucleotide sequence from the Rat Genome Database to the NCBI GenBank?
Which of the following steps is not mentioned in the text for primer design using Primer3?
Which of the following steps is not mentioned in the text for primer design using Primer3?
What should be avoided in the 3' end of a primer to prevent primer-dimer formation?
What should be avoided in the 3' end of a primer to prevent primer-dimer formation?
Why is it mentioned to increase the primer setting by 1 if no primers are returned?
Why is it mentioned to increase the primer setting by 1 if no primers are returned?
Which action should be taken after selecting the primers for testing?
Which action should be taken after selecting the primers for testing?
What could promoting mispriming at C or C-rich sequences potentially result in?
What could promoting mispriming at C or C-rich sequences potentially result in?
Why is it important to check the E-value after running BLAST on the primers?
Why is it important to check the E-value after running BLAST on the primers?
What should be done if there are no results after setting the value to 1 during primer selection?
What should be done if there are no results after setting the value to 1 during primer selection?
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