Primary Culture and Tissue Initiation Quiz

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Questions and Answers

What is the stage of a culture after isolation of cells but before the first subculture?

  • Secondary culture
  • Primary culture (correct)
  • Tertiary culture
  • Cell line culture

Which legislation regulates the use of embryos or fetuses beyond 50% gestation in the UK?

  • Animal Experiments (Scientific Procedures) Act of 1986 (correct)
  • EU Directives 2010
  • Office of Laboratory Animal Welfare [OLAW, 2013]
  • UPM regulations

At what age are most organs completely formed in a mouse embryo?

  • 9th day
  • 18th day (correct)
  • 13-14 days
  • 21st day

What is required for working with human biopsies or fetal material?

<p>Consent of the local ethical committee and the patient, and adherence to national regulation (D)</p> Signup and view all the answers

What is the stage of a culture after the first subculture, at which it becomes a continuous cell line?

<p>Cell line culture (B)</p> Signup and view all the answers

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