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Primary Cell Cultures vs Adherent Cell Cultures

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Primary Cell Cultures vs Adherent Cell Cultures

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Questions and Answers

What is the purpose of using protease/chelator solution in cell culture?

To induce division in connective tissue fibroblasts

To prevent cells from adhering to the culture dish

To remove adherent cells from the culture dish (correct)

To promote the growth of nonadherent cells

What happens if special precautions are not taken when isolating other types of cells from rapidly dividing connective tissue fibroblasts?

Other cells start dividing more rapidly

Special precautions are unnecessary

Connective tissue fibroblasts die off

Connective tissue fibroblasts become the predominant cell type (correct)

Why do certain cells from blood, spleen, or bone marrow grow well even though they adhere poorly to the culture dish?

Because they are held in suspension in the body's fluids (correct)

Because they are highly resistant to protease/chelator solutions

Because they require special nutrients to grow

Because they are non-dividing cells

What is a cell strain?

A lineage of cells originating from one initial primary culture Signup and view all the answers

What is a characteristic feature of an immortal cell line?

It has an indefinite life span and can grow indefinitely Signup and view all the answers

In what year was the HeLa cell line originally obtained?

1952 Signup and view all the answers

What is the characteristic of primary cell cultures?

They come from migrating cells and are morphologically similar to the parent tissue Signup and view all the answers

What happens to primary cells after a limited number of cell divisions?

They enter a non-proliferative state called senescence and eventually die out Signup and view all the answers

How are adherent cell cultures defined?

They attach to the surface of the culture vessel forming a monolayer Signup and view all the answers

What is the growth characteristic of suspension culture cells?

They are free-floating and do not attach to the surface Signup and view all the answers

What is required to prepare tissue cells for a primary culture?

Treatment with protease and EDTA to break cell-cell and cell-matrix interactions Signup and view all the answers

Why are released cells placed in dishes in a nutrient-rich, serum supplemented medium after preparation?

To allow cells to adhere to the surface and each other Signup and view all the answers