Podcast
Questions and Answers
What is the purpose of using protease/chelator solution in cell culture?
What is the purpose of using protease/chelator solution in cell culture?
- To induce division in connective tissue fibroblasts
- To prevent cells from adhering to the culture dish
- To remove adherent cells from the culture dish (correct)
- To promote the growth of nonadherent cells
What happens if special precautions are not taken when isolating other types of cells from rapidly dividing connective tissue fibroblasts?
What happens if special precautions are not taken when isolating other types of cells from rapidly dividing connective tissue fibroblasts?
- Other cells start dividing more rapidly
- Special precautions are unnecessary
- Connective tissue fibroblasts die off
- Connective tissue fibroblasts become the predominant cell type (correct)
Why do certain cells from blood, spleen, or bone marrow grow well even though they adhere poorly to the culture dish?
Why do certain cells from blood, spleen, or bone marrow grow well even though they adhere poorly to the culture dish?
- Because they are held in suspension in the body's fluids (correct)
- Because they are highly resistant to protease/chelator solutions
- Because they require special nutrients to grow
- Because they are non-dividing cells
What is a cell strain?
What is a cell strain?
What is a characteristic feature of an immortal cell line?
What is a characteristic feature of an immortal cell line?
In what year was the HeLa cell line originally obtained?
In what year was the HeLa cell line originally obtained?
What is the characteristic of primary cell cultures?
What is the characteristic of primary cell cultures?
What happens to primary cells after a limited number of cell divisions?
What happens to primary cells after a limited number of cell divisions?
How are adherent cell cultures defined?
How are adherent cell cultures defined?
What is the growth characteristic of suspension culture cells?
What is the growth characteristic of suspension culture cells?
What is required to prepare tissue cells for a primary culture?
What is required to prepare tissue cells for a primary culture?
Why are released cells placed in dishes in a nutrient-rich, serum supplemented medium after preparation?
Why are released cells placed in dishes in a nutrient-rich, serum supplemented medium after preparation?
