Practical Physiology Lecture 3

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27 Questions

What is the typical shape of the nucleus in Eosinophils?

Bilobed

What is the color of the cytoplasmic granules in Basophils?

Deep purple

What is unique about the shape of Monocytes?

Kidney-shaped

What is the characteristic of the cytoplasm in Monocytes?

Non-granular

What is the color of the nucleus in Eosinophils?

Blue-violet

Which of the following is NOT a characteristic of Basophils?

Tri-lobed nucleus

What is the name of the university where the Anesthesia Technology Department is located?

Al-Noor University

Who is the assistant lecturer in the Anesthesia Technology Department?

Asst.Lec. Mustafa Ahmed

What is the topic of the lecture?

Practical Physiology

What is the number of the lecture?

Lecture 3

What is the name of the person associated with the Anesthesia Technology Department?

Mustafa Ahmed Elias

What is the primary purpose of preparing a blood smear?

To observe the morphology of blood cells

What is the name of the stain used in the procedure?

Leishman stain

What is the source of the sample used in the procedure?

Blood sample

Where can the procedure of preparing a blood smear and staining it with Leishman be found?

In the previous lecture

What is the benefit of staining the blood smear with Leishman?

To enhance the visibility of blood cells

What type of infection is associated with an increase in eosinophils?

Allergy and parasitic infestation

What is the characteristic feature of neutrophils' nucleus?

Blue-purple, 3-5 lobed, and connected by chromatin threads

Which type of infection causes an increase in lymphocytes?

Viral infection

What is the characteristic feature of neutrophils' cytoplasm?

Small, pink, granular

Which type of leukemia is associated with an increase in monocytes?

Monocytic leukemia

What is the characteristic feature of appendicitis?

Increased neutrophils

What is the purpose of counting 100 WBCs on the slide?

To calculate the percentage of each type of WBC

What is the tool used to record the number of WBCs counted?

Differential cell counter

What is the next step after counting 100 WBCs?

To calculate the percentage of each type of WBC

What is the significance of the number 100 in the WBC counting process?

It is the target number of WBCs to be counted for calculation

What is the final product of the WBC counting and calculation process?

A percentage of each type of WBC

Study Notes

White Blood Cells (WBCs)

  • Bacterial infections, appendicitis, and allergy cause an increase in neutrophils
  • Parasitic infestation and allergy cause an increase in eosinophils
  • Viral infections cause an increase in lymphocytes
  • Monocytic leukemia causes an increase in monocytes

Characteristics of WBCs

  • Neutrophils: have a 3-5 lobed blue-purple nucleus, small pink cytoplasmic granules, and chromatin threads
  • Eosinophils: usually have a blue-violet bilobed nucleus, rarely 3-lobed, red-orange coarse cytoplasmic granules, and chromatin threads
  • Basophils: have an irregular shape, may be S-shaped, blue-violet un-segmented bilobed nucleus, rarely 3-lobed, and deep blue or deep purple cytoplasmic granules
  • Monocytes: are the largest cells in normal blood, have a kidney-shaped form, large pale blue-violet nucleus, and non-granular cytoplasm

Preparation of Blood Smear and Staining

  • Prepare a blood smear and stain it with Leishman stain as described in the previous lecture

Calculations

  • Count each WBC seen on the slide and record the number on a differential cell counter until 100 WBCs are counted
  • Calculate the percentage of each type of WBC by dividing the number of each type by 100

This quiz is based on the lecture 3 of Practical Physiology, part of Anesthesia Technology course at AL Noor University. It covers the key concepts and topics discussed in the lecture.

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