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Questions and Answers
What are plasmids?
What are plasmids?
- Extrachromosomal replicons found in eukaryotes.
- Chromosomal replicons found in prokaryotes.
- Extrachromosomal replicons found in prokaryotes. (correct)
- Chromosomal replicons found in eukaryotes.
How can plasmids exist?
How can plasmids exist?
- Only as single-stranded DNA molecules.
- Only as linear DNA molecules.
- Only as circular DNA molecules.
- Both as circular or linear DNA molecules. (correct)
What do plasmids control?
What do plasmids control?
- Only toxin production.
- Only the replication of chromosomal DNA
- Various phenotypes, including antibiotic resistance and toxin production. (correct)
- Only antibiotic resistance
Which plasmid is commonly used in cloning?
Which plasmid is commonly used in cloning?
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What do pUC vectors contain?
What do pUC vectors contain?
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What is plant transformation?
What is plant transformation?
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What is Agrobacterium-mediated gene transfer?
What is Agrobacterium-mediated gene transfer?
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What does Agrobacterium induce in plants during gene transfer?
What does Agrobacterium induce in plants during gene transfer?
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What is the Ti-plasmid necessary for?
What is the Ti-plasmid necessary for?
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What does A. rhizogenes use to induce adventitious root formation in plants?
What does A. rhizogenes use to induce adventitious root formation in plants?
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Study Notes
- Plasmids are extrachromosomal replicons found in prokaryotes.
- They can exist as circular or linear DNA molecules.
- Plasmids control various phenotypes, including antibiotic resistance and toxin production.
- pBR322 is a common plasmid used in cloning, with improved derivatives created to add unique restriction sites and selectable markers.
- pUC vectors contain multiple cloning sites and a DNA sequence for visual detection of an insert.
- Plant transformation involves introducing foreign genes into the host organism.
- Agrobacterium-mediated gene transfer is a common method for plant transformation.
- Agrobacterium induces the synthesis of opines in plants, which it can catabolize and use as a carbon and nitrogen source.
- The Ti-plasmid is necessary for A. tumefaciens to cause crown gall disease in plants.
- A. rhizogenes uses a Ri-plasmid to induce adventitious root formation in plants.
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