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Plasmids

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Questions and Answers

What are plasmids?

Extrachromosomal replicons found in eukaryotes.

Chromosomal replicons found in prokaryotes.

Extrachromosomal replicons found in prokaryotes. (correct)

Chromosomal replicons found in eukaryotes.

How can plasmids exist?

Only as single-stranded DNA molecules.

Only as linear DNA molecules.

Only as circular DNA molecules.

Both as circular or linear DNA molecules. (correct)

What do plasmids control?

Only toxin production.

Only the replication of chromosomal DNA

Various phenotypes, including antibiotic resistance and toxin production. (correct)

Only antibiotic resistance

Which plasmid is commonly used in cloning?

pBR322 Signup and view all the answers

What do pUC vectors contain?

Multiple cloning sites and a DNA sequence for visual detection of an insert. Signup and view all the answers

What is plant transformation?

Introducing foreign genes into the host organism. Signup and view all the answers

What is Agrobacterium-mediated gene transfer?

A common method for plant transformation. Signup and view all the answers

What does Agrobacterium induce in plants during gene transfer?

The synthesis of opines. Signup and view all the answers

What is the Ti-plasmid necessary for?

A) tumefaciens to cause crown gall disease in plants. Signup and view all the answers

What does A. rhizogenes use to induce adventitious root formation in plants?

Ri-plasmid Signup and view all the answers

Study Notes

Plasmids are extrachromosomal replicons found in prokaryotes.
They can exist as circular or linear DNA molecules.
Plasmids control various phenotypes, including antibiotic resistance and toxin production.
pBR322 is a common plasmid used in cloning, with improved derivatives created to add unique restriction sites and selectable markers.
pUC vectors contain multiple cloning sites and a DNA sequence for visual detection of an insert.
Plant transformation involves introducing foreign genes into the host organism.
Agrobacterium-mediated gene transfer is a common method for plant transformation.
Agrobacterium induces the synthesis of opines in plants, which it can catabolize and use as a carbon and nitrogen source.
The Ti-plasmid is necessary for A. tumefaciens to cause crown gall disease in plants.
A. rhizogenes uses a Ri-plasmid to induce adventitious root formation in plants.

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Description

Test your knowledge on plasmids and plant transformation with this informative quiz! Learn about the structure, function, and common uses of plasmids in cloning, as well as the methods and mechanisms behind plant transformation. From pBR322 to Agrobacterium-mediated gene transfer, explore the fascinating world of plasmids and their impact on genetic engineering.