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Plasmid Minipreparation Quiz

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Questions and Answers

What is the main objective of plasmid minipreparation?

To differentiate between bacterial chromosome and plasmid

To isolate bacterial chromosome from plasmid DNA

To extract plasmid DNA from bacterial cell suspensions (correct)

To replicate plasmid DNA independently

Which of the following is a characteristic of plasmid DNA?

It is a part of the bacterial chromosome

It is larger and less supercoiled than bacterial chromosomal DNA

It cannot replicate independently

It is physically separated from chromosomal DNA (correct)

What makes plasmid DNA different from bacterial chromosomal DNA?

It can replicate independently (correct)

It cannot be separated from chromosomal DNA

It is larger and more supercoiled

It is located within the bacterial chromosome

How does the method of plasmid minipreparation take advantage of the differences between plasmids and bacterial chromosomal DNA?

By utilizing alkaline lysis to isolate supercoiled plasmid DNA Signup and view all the answers

Why is plasmid minipreparation method advantageous for isolating plasmid DNA?

Plasmids are relatively small and supercoiled compared to larger, less supercoiled bacterial chromosomal DNA Signup and view all the answers

What characteristic of plasmid DNA makes it suitable for the minipreparation method?

Small size and supercoiling Signup and view all the answers

In comparison to bacterial chromosomal DNA, what distinguishes plasmid DNA in the minipreparation method?

Smaller size and higher supercoiling Signup and view all the answers

Why is it feasible to isolate plasmid DNA using the minipreparation method?

Plasmids are small, supercoiled DNA molecules, making them distinguishable from larger, less supercoiled bacterial chromosomal DNA Signup and view all the answers

What allows for selective precipitation of the chromosomal DNA from plasmids and RNA molecules in plasmid minipreparation?

Difference in topology Signup and view all the answers

In plasmid minipreparation, why is it advantageous to selectively precipitate the chromosomal DNA from plasmids and RNA molecules?

To isolate pure plasmid DNA Signup and view all the answers

What differentiates plasmid DNA from bacterial chromosomal DNA in the context of plasmid minipreparation?

Topology Signup and view all the answers

Which characteristic of plasmid DNA makes it suitable for the selective precipitation in plasmid minipreparation?

Circular structure Signup and view all the answers