Podcast
Questions and Answers
What is the main objective of plasmid minipreparation?
What is the main objective of plasmid minipreparation?
- To differentiate between bacterial chromosome and plasmid
- To isolate bacterial chromosome from plasmid DNA
- To extract plasmid DNA from bacterial cell suspensions (correct)
- To replicate plasmid DNA independently
Which of the following is a characteristic of plasmid DNA?
Which of the following is a characteristic of plasmid DNA?
- It is a part of the bacterial chromosome
- It is larger and less supercoiled than bacterial chromosomal DNA
- It cannot replicate independently
- It is physically separated from chromosomal DNA (correct)
What makes plasmid DNA different from bacterial chromosomal DNA?
What makes plasmid DNA different from bacterial chromosomal DNA?
- It can replicate independently (correct)
- It cannot be separated from chromosomal DNA
- It is larger and more supercoiled
- It is located within the bacterial chromosome
How does the method of plasmid minipreparation take advantage of the differences between plasmids and bacterial chromosomal DNA?
How does the method of plasmid minipreparation take advantage of the differences between plasmids and bacterial chromosomal DNA?
Why is plasmid minipreparation method advantageous for isolating plasmid DNA?
Why is plasmid minipreparation method advantageous for isolating plasmid DNA?
What characteristic of plasmid DNA makes it suitable for the minipreparation method?
What characteristic of plasmid DNA makes it suitable for the minipreparation method?
In comparison to bacterial chromosomal DNA, what distinguishes plasmid DNA in the minipreparation method?
In comparison to bacterial chromosomal DNA, what distinguishes plasmid DNA in the minipreparation method?
Why is it feasible to isolate plasmid DNA using the minipreparation method?
Why is it feasible to isolate plasmid DNA using the minipreparation method?
What allows for selective precipitation of the chromosomal DNA from plasmids and RNA molecules in plasmid minipreparation?
What allows for selective precipitation of the chromosomal DNA from plasmids and RNA molecules in plasmid minipreparation?
In plasmid minipreparation, why is it advantageous to selectively precipitate the chromosomal DNA from plasmids and RNA molecules?
In plasmid minipreparation, why is it advantageous to selectively precipitate the chromosomal DNA from plasmids and RNA molecules?
What differentiates plasmid DNA from bacterial chromosomal DNA in the context of plasmid minipreparation?
What differentiates plasmid DNA from bacterial chromosomal DNA in the context of plasmid minipreparation?
Which characteristic of plasmid DNA makes it suitable for the selective precipitation in plasmid minipreparation?
Which characteristic of plasmid DNA makes it suitable for the selective precipitation in plasmid minipreparation?
