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Plasmid Purification with Alkaline Lysis

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Plasmid Purification with Alkaline Lysis

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Questions and Answers

Which of the following is true about plasmids?

They are only found in superbugs.

They are encoded on the bacterial chromosome.

They are pieces of DNA that carry genetic information in bacterial cells. (correct)

They are resistant to multiple antibiotics.

What is the purpose of Solution I in plasmid purification?

To resuspend the plasmid after removal of LB broth. (correct)

To reduce the electrostatic interactions of DNA.

To lyse the bacterial cells.

To bind any free metal ions.

Which component of Solution II helps in lysing the bacterial cells?

EDTA

Tris (pH8)

SDS (detergent) (correct)

CH3COONa

What is the role of dextrose/glucose in Solution I?

<p>To prevent cell lysing from osmotic shock.</p> Signup and view all the answers

What is the purpose of EDTA in Solution I?

<p>To bind any free metal ions.</p> Signup and view all the answers

Study Notes

Plasmid Purification

Plasmids are true replicons that exist independently of the chromosomal DNA in bacteria.

Solution I

Purpose: To create a conducive environment for bacterial cells to undergo lysis.
Role of dextrose/glucose: To create a hypertonic environment that prevents the bacterial cells from lysing prematurely.
Role of EDTA: To inactivate metal-dependent enzymes that could degrade DNA, and to help maintain the integrity of the plasmid.

Solution II

Component that helps in lysing the bacterial cells: SDS (Sodium Dodecyl Sulfate)

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Description

Test your knowledge on plasmid purification with alkaline lysis! Learn about the extrachromosomal pieces of DNA called plasmids that carry genetic information in bacterial cells. Discover how plasmids enhance the survival abilities of bacteria under selective pressures and confer antibiotic resistance. Explore the concept of superbugs, which are bacteria resistant to multiple antibiotics, and understand how plasmids play a role in this. Increase your understanding of plasmids and their separation through this informative quiz.