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Questions and Answers
Which of the following is true about plasmids?
Which of the following is true about plasmids?
- They are only found in superbugs.
- They are encoded on the bacterial chromosome.
- They are pieces of DNA that carry genetic information in bacterial cells. (correct)
- They are resistant to multiple antibiotics.
What is the purpose of Solution I in plasmid purification?
What is the purpose of Solution I in plasmid purification?
- To resuspend the plasmid after removal of LB broth. (correct)
- To reduce the electrostatic interactions of DNA.
- To lyse the bacterial cells.
- To bind any free metal ions.
Which component of Solution II helps in lysing the bacterial cells?
Which component of Solution II helps in lysing the bacterial cells?
- EDTA
- Tris (pH8)
- SDS (detergent) (correct)
- CH3COONa
What is the role of dextrose/glucose in Solution I?
What is the role of dextrose/glucose in Solution I?
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What is the purpose of EDTA in Solution I?
What is the purpose of EDTA in Solution I?
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Study Notes
Plasmid Purification
- Plasmids are true replicons that exist independently of the chromosomal DNA in bacteria.
Solution I
- Purpose: To create a conducive environment for bacterial cells to undergo lysis.
- Role of dextrose/glucose: To create a hypertonic environment that prevents the bacterial cells from lysing prematurely.
- Role of EDTA: To inactivate metal-dependent enzymes that could degrade DNA, and to help maintain the integrity of the plasmid.
Solution II
- Component that helps in lysing the bacterial cells: SDS (Sodium Dodecyl Sulfate)
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