18 Questions
What can be added to the culture medium to overcome variation due to callusing?
Triiodobenzoic acid (TIBA) and phloroglucinol
Why do tissue culture regenerated plants need hardening?
To prevent transplantation shock
What is a limitation of micro propagation in plants with high levels of phenols?
Unresponsiveness to micro propagation techniques
What is a method used for hardening of in vitro raised plants?
Partial defoliation
Why is technical skill required in micro propagation?
To perform different micro propagation procedures
What can be applied to in vitro raised plants to prevent water loss during hardening?
Antitranspirants
What is the purpose of fumigation of the inoculation room using dilute formaldehyde solution?
To minimize the growth of contaminants
Why are plants regenerated through adventitious meristems more susceptible to mutations?
Because they are derived from a single cell or a small group of cells
What is the purpose of using antioxidants such as activated charcoal in the culture medium?
To prevent browning of cultures
Why is direct shoot induction and proliferation preferred over callusing and regeneration?
Because it is less susceptible to mutations
What is the purpose of using growth chambers for growing donor plants?
To minimize the growth of contaminants
Why is variability in T/C regenerated plants undesirable?
Because it affects the consistency and uniformity of the plants
What is the primary advantage of micro propagation in plant breeding?
It enables the rapid and large-scale clonal multiplication of elite germplasm
What is the typical size of the meristem explant used in micro propagation?
0.1-0.5 mm
Who first demonstrated the potential of micro propagation for producing virus-free plants?
Morel and Martin
What was the significance of the discovery of hormonal control of organogenesis by Shoog and Miller?
It extended the scope of micro propagation to a vast range of plant species
Approximately how many plants are produced annually through micro propagation worldwide?
500 million
Which country is the largest producer of micro-propagated plants?
United States
Learn about the techniques and methods used to overcome challenges in plant tissue culture, including contamination and browning of cultures. Understand the importance of sterilization, fumigation, and controlled environments in plant tissue culture.
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