Plant Tissue Culture Techniques

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Questions and Answers

What can be added to the culture medium to overcome variation due to callusing?

  • Plant growth promoters
  • Hormone regulators
  • Triiodobenzoic acid (TIBA) and phloroglucinol (correct)
  • Inorganic salts

Why do tissue culture regenerated plants need hardening?

  • To increase photosynthetic efficiency
  • To promote stomatal malfunction
  • To reduce epicuticular waxes
  • To prevent transplantation shock (correct)

What is a limitation of micro propagation in plants with high levels of phenols?

  • Technical skill requirement
  • Unresponsiveness to micro propagation techniques (correct)
  • Pathogen multiplication
  • High cost of facilities

What is a method used for hardening of in vitro raised plants?

<p>Partial defoliation (D)</p> Signup and view all the answers

Why is technical skill required in micro propagation?

<p>To perform different micro propagation procedures (A)</p> Signup and view all the answers

What can be applied to in vitro raised plants to prevent water loss during hardening?

<p>Antitranspirants (B)</p> Signup and view all the answers

What is the purpose of fumigation of the inoculation room using dilute formaldehyde solution?

<p>To minimize the growth of contaminants (A)</p> Signup and view all the answers

Why are plants regenerated through adventitious meristems more susceptible to mutations?

<p>Because they are derived from a single cell or a small group of cells (D)</p> Signup and view all the answers

What is the purpose of using antioxidants such as activated charcoal in the culture medium?

<p>To prevent browning of cultures (A)</p> Signup and view all the answers

Why is direct shoot induction and proliferation preferred over callusing and regeneration?

<p>Because it is less susceptible to mutations (B)</p> Signup and view all the answers

What is the purpose of using growth chambers for growing donor plants?

<p>To minimize the growth of contaminants (D)</p> Signup and view all the answers

Why is variability in T/C regenerated plants undesirable?

<p>Because it affects the consistency and uniformity of the plants (C)</p> Signup and view all the answers

What is the primary advantage of micro propagation in plant breeding?

<p>It enables the rapid and large-scale clonal multiplication of elite germplasm (D)</p> Signup and view all the answers

What is the typical size of the meristem explant used in micro propagation?

<p>0.1-0.5 mm (C)</p> Signup and view all the answers

Who first demonstrated the potential of micro propagation for producing virus-free plants?

<p>Morel and Martin (B)</p> Signup and view all the answers

What was the significance of the discovery of hormonal control of organogenesis by Shoog and Miller?

<p>It extended the scope of micro propagation to a vast range of plant species (C)</p> Signup and view all the answers

Approximately how many plants are produced annually through micro propagation worldwide?

<p>500 million (B)</p> Signup and view all the answers

Which country is the largest producer of micro-propagated plants?

<p>United States (D)</p> Signup and view all the answers

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