Plant Tissue Culture Techniques
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Questions and Answers

What can be added to the culture medium to overcome variation due to callusing?

  • Plant growth promoters
  • Hormone regulators
  • Triiodobenzoic acid (TIBA) and phloroglucinol (correct)
  • Inorganic salts
  • Why do tissue culture regenerated plants need hardening?

  • To increase photosynthetic efficiency
  • To promote stomatal malfunction
  • To reduce epicuticular waxes
  • To prevent transplantation shock (correct)
  • What is a limitation of micro propagation in plants with high levels of phenols?

  • Technical skill requirement
  • Unresponsiveness to micro propagation techniques (correct)
  • Pathogen multiplication
  • High cost of facilities
  • What is a method used for hardening of in vitro raised plants?

    <p>Partial defoliation</p> Signup and view all the answers

    Why is technical skill required in micro propagation?

    <p>To perform different micro propagation procedures</p> Signup and view all the answers

    What can be applied to in vitro raised plants to prevent water loss during hardening?

    <p>Antitranspirants</p> Signup and view all the answers

    What is the purpose of fumigation of the inoculation room using dilute formaldehyde solution?

    <p>To minimize the growth of contaminants</p> Signup and view all the answers

    Why are plants regenerated through adventitious meristems more susceptible to mutations?

    <p>Because they are derived from a single cell or a small group of cells</p> Signup and view all the answers

    What is the purpose of using antioxidants such as activated charcoal in the culture medium?

    <p>To prevent browning of cultures</p> Signup and view all the answers

    Why is direct shoot induction and proliferation preferred over callusing and regeneration?

    <p>Because it is less susceptible to mutations</p> Signup and view all the answers

    What is the purpose of using growth chambers for growing donor plants?

    <p>To minimize the growth of contaminants</p> Signup and view all the answers

    Why is variability in T/C regenerated plants undesirable?

    <p>Because it affects the consistency and uniformity of the plants</p> Signup and view all the answers

    What is the primary advantage of micro propagation in plant breeding?

    <p>It enables the rapid and large-scale clonal multiplication of elite germplasm</p> Signup and view all the answers

    What is the typical size of the meristem explant used in micro propagation?

    <p>0.1-0.5 mm</p> Signup and view all the answers

    Who first demonstrated the potential of micro propagation for producing virus-free plants?

    <p>Morel and Martin</p> Signup and view all the answers

    What was the significance of the discovery of hormonal control of organogenesis by Shoog and Miller?

    <p>It extended the scope of micro propagation to a vast range of plant species</p> Signup and view all the answers

    Approximately how many plants are produced annually through micro propagation worldwide?

    <p>500 million</p> Signup and view all the answers

    Which country is the largest producer of micro-propagated plants?

    <p>United States</p> Signup and view all the answers

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