Podcast Beta
Questions and Answers
What is tissue culture?
What factors are included in the controlled conditions for tissue culture?
What is a key advantage of plant tissue culture technology?
What is the purpose of callus cultures in plant tissue culture?
Signup and view all the answers
What is the significance of Murashige and Skoog medium (MS medium) in plant tissue culture?
Signup and view all the answers
What is the historical significance of the discovery of the first plant growth hormone in 1926?
Signup and view all the answers
Which technique has been used for the somatic embryogenesis and plant regeneration of Jucara Palm?
Signup and view all the answers
What is the purpose of haploid plants conversion into homozygous diploids?
Signup and view all the answers
Which method has led to successful transgenic plants of Jatropha, impacting the reduction of toxic substances in seeds?
Signup and view all the answers
What is the typical pH range for plant tissue culture medium?
Signup and view all the answers
Which plant growth regulators play a crucial role in determining the development pathway of plant cells and tissues in culture medium?
Signup and view all the answers
What is the purpose of cryopreservation in liquid nitrogen in plant tissue culture?
Signup and view all the answers
What is the purpose of tissue culture techniques in plant cell biotechnology?
Signup and view all the answers
What is the main advantage of transgenic plants over fermentation-based production systems?
Signup and view all the answers
What are the stages involved in micropropagation?
Signup and view all the answers
Study Notes
Current and Future Status of Plant Tissue Culture
- Plant cell biotechnology has evolved as a new era in the field of biotechnology, focusing on the production of a large number of secondary plant products.
- Genetic engineering and molecular biology techniques have allowed the development of improved and new agricultural products, leading to an increasing demand in several countries worldwide.
- Tissue culture techniques provided the tools for the introduction of genetic information into plant cells, enabling the production of proteins and medicinal substances in transgenic plants.
- Transgenic plants represent an economical alternative to fermentation-based production systems and are especially promising for producing vaccines and antibodies.
- The number of farmers who incorporated transgenic plants into their production systems was 13.3 million in 2008, compared to 11 million in 2007.
- Micropropagation starts with the selection of plant tissues (explant) from a healthy, vigorous mother plant, and any part of the plant can be used as explant.
- The stages of micropropagation include preparation of the donor plant, initiation stage, multiplication stage, rooting stage, and acclimatization stage.
- Somatic embryogenesis is an in vitro method of plant regeneration widely used for sustained clonal propagation, involving somatic cells or tissues developing into differentiated embryos.
- Somatic embryogenesis can be initiated directly from the explants or indirectly by the establishment of mass of unorganized cells named callus.
- Plant regeneration via organogenesis involves the production of plant organs directly from the meristem or indirectly from undifferentiated cell masses (callus).
- Somatic embryogenesis and organogenesis are valuable tools for genetic manipulation, developing plants resistant to various stresses, and introducing genes by genetic transformation.
- Factors such as plant growth regulators and a highly efficient protocol have been reported to affect the induction and development of somatic embryos in cultured cells.
Studying That Suits You
Use AI to generate personalized quizzes and flashcards to suit your learning preferences.
Description
Test your knowledge of plant tissue culture with this quiz. Explore topics such as genetic engineering, transgenic plants, micropropagation, somatic embryogenesis, and organogenesis. Learn about the current and future status of plant tissue culture in biotechnology and agriculture.
