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Questions and Answers
Which components are included in Plant Tissue Culture for plant growth?
What are the main inorganic salt ions essential for plant growth in Plant Tissue Culture?
Which compounds are used as callus-inducing agents in Plant Tissue Culture?
What is the role of hexitols in Plant Tissue Culture?
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What are the examples of carbohydrates used in Plant Tissue Culture?
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What is an explant in plant tissue culture?
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What is the significance of physiological age of the organ in explant selection?
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How does the season affect explant choice?
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What are the factors affecting explant choice?
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What can callus formation lead to in plant tissue culture?
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Study Notes
Plant Tissue Culture Components
- Plant Tissue Culture includes several components, including a carbon source, inorganic salts, growth regulators, vitamins, and amino acids, to support plant growth.
Essential Inorganic Salt Ions
- Inorganic salt ions essential for plant growth in Plant Tissue Culture include calcium, magnesium, potassium, and ammonium, which are involved in various physiological processes.
Callus-Inducing Agents
- Compounds used as callus-inducing agents in Plant Tissue Culture include auxins, such as 2,4-dichlorophenoxyacetic acid (2,4-D), and cytokinins, which promote cell division and differentiation.
Hexitols in Plant Tissue Culture
- Hexitols, such as sorbitol and mannitol, are used in Plant Tissue Culture to maintain osmotic balance and prevent water stress in plant cells.
Carbohydrates in Plant Tissue Culture
- Examples of carbohydrates used in Plant Tissue Culture include sucrose, glucose, and fructose, which serve as a source of carbon and energy for plant growth.
Explant in Plant Tissue Culture
- An explant in plant tissue culture is a piece of tissue or an organ taken from a plant and used to initiate tissue culture.
Physiological Age and Explant Selection
- The physiological age of the organ is significant in explant selection, as it affects the explant's ability to respond to tissue culture conditions and produce callus.
Seasonal Effects on Explant Choice
- The season can affect explant choice, as certain explants may be more or less responsive to tissue culture conditions during specific times of the year.
Factors Affecting Explant Choice
- Factors affecting explant choice include the physiological age of the organ, the type of plant, and the desired response to tissue culture conditions.
Callus Formation
- Callus formation in plant tissue culture can lead to the development of somatic embryos, adventitious shoots, and roots, and can be used for plant propagation and genetic modification.
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Description
Test your knowledge on the essential components and techniques used in plant tissue culture, including inorganic components, growth regulators, and nutritional requirements.
