Plant Tissue Culture Essentials Quiz
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Questions and Answers

Which components are included in Plant Tissue Culture for plant growth?

  • Organic components, plant growth regulators, minerals, nucleic acids
  • Inorganic components, plant growth regulators, vitamins, carbohydrates, hexitols (correct)
  • Organic components, animal growth regulators, minerals, lipids
  • Inorganic components, animal growth regulators, vitamins, proteins

    • What are the main inorganic salt ions essential for plant growth in Plant Tissue Culture?

  • Chlorine (Cl), nickel (Ni), molybdenum (Mo), iodine (I), fluorine (F), selenium (Se)
  • Sodium (Na), iron (Fe), zinc (Zn), copper (Cu), manganese (Mn), boron (B)
  • Carbon (C), hydrogen (H), oxygen (O), nitrogen (N), phosphorus (P), sulphur (S)
  • Nitrogen (N), potassium (K), calcium (Ca), phosphorus (P), magnesium (Mg), sulphur (S) (correct)

    • Which compounds are used as callus-inducing agents in Plant Tissue Culture?

  • BA
  • Cytokinins
  • IAA
  • 2,4-D (correct)

    • What is the role of hexitols in Plant Tissue Culture?

    <p>Storage of polyhydric compounds and involvement in reserves and sugar transport</p> Signup and view all the answers

    What are the examples of carbohydrates used in Plant Tissue Culture?

    <p>Glucose and sucrose</p> Signup and view all the answers

    What is an explant in plant tissue culture?

    <p>A piece of plant tissue placed into tissue culture medium for generating a whole new plant</p> Signup and view all the answers

    What is the significance of physiological age of the organ in explant selection?

    <p>Physiologically younger tissue is generally more responsive in vitro</p> Signup and view all the answers

    How does the season affect explant choice?

    <p>Buds or shoots taken during the spring when in a flush state of growth are more responsive</p> Signup and view all the answers

    What are the factors affecting explant choice?

    <p>Physiological age, season, size, location, quality of the source plant, ultimate goal, and plant genotype</p> Signup and view all the answers

    What can callus formation lead to in plant tissue culture?

    <p>The transformation into shoot and root, hence a new plant</p> Signup and view all the answers

    Study Notes

    Plant Tissue Culture Components

    • Plant Tissue Culture includes several components, including a carbon source, inorganic salts, growth regulators, vitamins, and amino acids, to support plant growth.

    Essential Inorganic Salt Ions

    • Inorganic salt ions essential for plant growth in Plant Tissue Culture include calcium, magnesium, potassium, and ammonium, which are involved in various physiological processes.

    Callus-Inducing Agents

    • Compounds used as callus-inducing agents in Plant Tissue Culture include auxins, such as 2,4-dichlorophenoxyacetic acid (2,4-D), and cytokinins, which promote cell division and differentiation.

    Hexitols in Plant Tissue Culture

    • Hexitols, such as sorbitol and mannitol, are used in Plant Tissue Culture to maintain osmotic balance and prevent water stress in plant cells.

    Carbohydrates in Plant Tissue Culture

    • Examples of carbohydrates used in Plant Tissue Culture include sucrose, glucose, and fructose, which serve as a source of carbon and energy for plant growth.

    Explant in Plant Tissue Culture

    • An explant in plant tissue culture is a piece of tissue or an organ taken from a plant and used to initiate tissue culture.

    Physiological Age and Explant Selection

    • The physiological age of the organ is significant in explant selection, as it affects the explant's ability to respond to tissue culture conditions and produce callus.

    Seasonal Effects on Explant Choice

    • The season can affect explant choice, as certain explants may be more or less responsive to tissue culture conditions during specific times of the year.

    Factors Affecting Explant Choice

    • Factors affecting explant choice include the physiological age of the organ, the type of plant, and the desired response to tissue culture conditions.

    Callus Formation

    • Callus formation in plant tissue culture can lead to the development of somatic embryos, adventitious shoots, and roots, and can be used for plant propagation and genetic modification.

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    Description

    Test your knowledge on the essential components and techniques used in plant tissue culture, including inorganic components, growth regulators, and nutritional requirements.

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