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Questions and Answers
Which medium is most widely used for many types of culture systems?
Which medium is most widely used for many types of culture systems?
- White's medium
- B5 medium
- N6 medium
- MS medium (correct)
Who developed the B5 medium used for protoplast culture?
Who developed the B5 medium used for protoplast culture?
- Chu
- Nitsch and Nitsch
- Gamborg (correct)
- Murashige and Skoog
What is N6 medium primarily used for?
What is N6 medium primarily used for?
- Cell suspension culture
- Anther culture in cereals (correct)
- Protoplast culture
- Callus cultures
Which medium was formulated for use in anther culture?
Which medium was formulated for use in anther culture?
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What is a characteristic of White's medium?
What is a characteristic of White's medium?
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Study Notes
Plant Tissue Culture Media
- White's medium is among the earliest plant tissue culture media.
- Murashige and Skoog (MS) formulated MS medium, which is the most widely utilized medium for diverse culture systems in plant biology.
Other Notable Media
- B5 medium, developed by Gamborg, is specifically for cell suspension and callus cultures; its modified versions are utilized in protoplast culture.
- N6 medium, created by Chu, is employed in cereal anther culture, showcasing its relevance in cereal plant studies.
- Nitsch's medium was developed by Nitsch and Nitsch, primarily for anther culture applications, indicating its significance in reproductive plant tissue studies.
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Description
This quiz covers the various types of plant tissue culture media, including Murashige and Skoog (MS) medium, Gamborg's B5 medium, and others like N6 and Nitsch’s mediums. Understand the formulations and applications of these essential media in plant biotechnology. Test your knowledge on the development and usage of these critical culture systems.
