Questions and Answers
In which temperature range does thermotherapy treatment involve keeping plants or plant parts for an appropriate period?
35°C and 54°C
What is cryotherapy, a new approach within thermotherapy, involve?
freezing shoot tips in liquid nitrogen and subsequent regeneration to shoots, resulting in virus-free plants with high efficiency
What percentage of trials involve plant thermotherapy as the most frequently applied sanitation technique?
54%
What are the key sources of information for the study?
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What are some of the abbreviations used in the paper?
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- What is the potential influence of temperature on Virus-Induced Gene Silencing (VIGS) in plants, as indicated by the text?
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- What are the differences in the effectiveness of thermotherapy against parenchymatic and phloematic viruses in plants, as mentioned in the text?
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- How has research in the past 20 years contributed to understanding plant defense mechanisms against viral infections?
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- What influential parameters beyond tissue localization have been suggested in the thermotherapy elimination of phloematic viruses in plants?
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- What is the importance of understanding temperature-dependent gene silencing mechanisms in effectively eliminating viral infections in plants, as demonstrated by the study by Chellapan et al. (2005)?
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Which technique is most frequently used in potato for eliminating viruses from plants according to the text?
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Name three groups of antiviral drugs mentioned in the text.
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Which method is reported to have successfully eradicated viruses belonging to the most families?
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What are the most frequent targets of sanitation protocols among woody plants?
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What does the interpretation of thermotherapy effects consider, with particular reference to virus-induced gene silencing?
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What is the role of thermotherapy in eliminating plant viruses?
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Which research study achieved similar results to virus-induced gene silencing (VIGS) when treating Cassava mosaic disease in Nicotiana benthamiana plants?
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What significant discovery in antiviral chemotherapy research is mentioned in the text?
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Which compounds were mentioned as effective against Tobacco mosaic virus (TMV) in the text?
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What combination of substances was effective in eliminating Grapevine vitivirus A?
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Study Notes
- The text discusses research on eliminating plant viruses using thermotherapy and chemotherapy.
- Thermotherapy involves exposing infected plants to high temperatures, which induces silencing of the virus.
- Cassavamosaic disease was studied on Nicotiana benthamiana plants infected with the disease, and thermotherapy achieved similar results to virus-induced gene silencing (VIGS).
- Qu et al. (2005) investigated the role of RNA-dependent RNA polymerase (RdRp) in silencing complex formation in N. benthamiana plants infected with Potato virus X.
- Wang et al. (2008) combined thermotherapy and cryotherapy to remove meristem tips from raspberry plants infected with Raspberry dwarf virus, noting the close relationship between temperature and RNA silencing.
- The development of antiviral chemotherapy in medicine provided a basis for research on phytoviruses.
- Ribavirin, a nucleoside analogue, was a significant discovery in antiviral chemotherapy research.
- Herbaceous plants have been more frequently studied than woody plants in chemotherapy trials.
- Potato, orchid, tobacco, grapevine, apple, and plum have been the most treated hosts.
- Nine families and an unassigned genus of viruses have been successfully treated with chemotherapy, using prodrugs such as ribavirin and synthetic nucleosides or non-nucleosides.
- Panattoni et al. (2005, 2007a) used synthetic nucleosides against Cucumber mosaic virus (CMV) and Grapevine leafroll-associated virus 3 (GLRaV-3), and obtained interesting results.
- Dihydroxypropyladenine in combination with ribavirin eliminated Grapevine vitivirus A.
- Oseltamivir was effective against CMV and GLRaV-3 in vitro.
- Bitriazolyl compounds, tylophorine B, phenanthrene-based tylophorine derivatives, derivatives of thiadiazole-acetamide, and cyanoacrylate derivatives were effective against Tobacco mosaic virus (TMV), but no sanitized plants were reported for these novel compounds.
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