Plant Pathogens and Disease Triangle Quiz

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The pathogen must be associated with the disease, the pathogen must be isolated and grown in pure culture, the pathogen must be inoculated into healthy plants (correct)
The pathogen must be associated with the disease, the pathogen must be grown in pure culture, the pathogen must be inoculated into healthy plants
The pathogen must be associated with the disease, the pathogen must be isolated and grown in pure culture, the pathogen must be re-isolated from inoculated plants
The pathogen must be isolated and grown in pure culture, the pathogen must be inoculated into healthy plants, the pathogen must be re-isolated from inoculated plants

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Robert Koch proved that Bacillus anthracis is responsible for anthrax in animals and humans.

Establish a correlation between the pathogen and the disease by isolating the microbe from a diseased organism.
Cultivate the microbe in pure culture and demonstrate that it causes the same disease when introduced to a healthy host.
Re-isolate the microbe from the experimentally infected host to confirm the pathogenicity.

Examples of biotic disorders include diseases caused by bacteria, viruses, fungi, and nematodes.

The disease triangle is also referred to as the 'pathogen-host-environment triangle', illustrating the interplay between these components in disease development.