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Questions and Answers
_______ Permit the transfer of accurately known volumes from one container to another.
_______ Permit the transfer of accurately known volumes from one container to another.
PIPETS
A volumetric, or transfer, pipet delivers a single, fixed volume between 0.5 and 200 Ml. Measuring Pipets are calibrated in convenient units to permit delivery of any volume up to a maximum capacity ranging from 0.1 to 25 Ml.
A volumetric, or transfer, pipet delivers a single, fixed volume between 0.5 and 200 Ml. Measuring Pipets are calibrated in convenient units to permit delivery of any volume up to a maximum capacity ranging from 0.1 to 25 Ml.
PIPETS
Transfer small quantities of liquids. Plastic bulb pipettes are typically not precise enough to be used for exact measurements but their glass counterparts can be extremely precise. The longer and thinner the tip of a glass pipette is the more exact the measurement.
Transfer small quantities of liquids. Plastic bulb pipettes are typically not precise enough to be used for exact measurements but their glass counterparts can be extremely precise. The longer and thinner the tip of a glass pipette is the more exact the measurement.
TRANSFER PIPETTES/PASTEUR PIPETTES
Have a plain, narrow tube which extends to a tip and graduates uniformly along its length. With a Mohr pipette, calibration marks may be confined to the stem.
Have a plain, narrow tube which extends to a tip and graduates uniformly along its length. With a Mohr pipette, calibration marks may be confined to the stem.
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______ College Medical Technology Department City of Koronadal, South Cotabato
______ College Medical Technology Department City of Koronadal, South Cotabato
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Air displacement is a fundamental principle that underlies the functioning of many types of ______
Air displacement is a fundamental principle that underlies the functioning of many types of ______
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When the plunger of the pipette is depressed to its first stop, a partial vacuum is created within the pipette's chamber above the ______
When the plunger of the pipette is depressed to its first stop, a partial vacuum is created within the pipette's chamber above the ______
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When the pipette is positioned over the target container, the plunger is further depressed to the second stop, expelling the liquid from the tip and into the target ______
When the pipette is positioned over the target container, the plunger is further depressed to the second stop, expelling the liquid from the tip and into the target ______
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Volumetric pipettes offer very accurate volume measurement of a solution. They have a large bulb with a long narrow portion. This portion has a single graduation mark, as it is calibrated for a single volume such as 10, 25, and 50 mL. These pipettes are commonly used in analytical chemistry to make laboratory solutions from a base stock as well as prepare solutions for ______.
Volumetric pipettes offer very accurate volume measurement of a solution. They have a large bulb with a long narrow portion. This portion has a single graduation mark, as it is calibrated for a single volume such as 10, 25, and 50 mL. These pipettes are commonly used in analytical chemistry to make laboratory solutions from a base stock as well as prepare solutions for ______.
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Single channel vs. multichannel pipettes are determined based on the total number of pistons in the pipette. For manual high-throughput lab applications, most would prefer a multichannel pipette instead of a single channel, which would require filling well by well. These pipettes are used for precise measurement of volume and dispensation in ______.
Single channel vs. multichannel pipettes are determined based on the total number of pistons in the pipette. For manual high-throughput lab applications, most would prefer a multichannel pipette instead of a single channel, which would require filling well by well. These pipettes are used for precise measurement of volume and dispensation in ______.
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Electronic pipettes were developed to improve ergonomics by reducing the necessary force. The manual movement of the piston is replaced by a small battery-powered electric motor. Whereas manual pipettes need a movement of the thumb (up to 3 cm), electronic pipettes use a main ______.
Electronic pipettes were developed to improve ergonomics by reducing the necessary force. The manual movement of the piston is replaced by a small battery-powered electric motor. Whereas manual pipettes need a movement of the thumb (up to 3 cm), electronic pipettes use a main ______.
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With a serologic pipette, they may extend to the tip. These pipettes operate on the principle of air displacement. Aspiration in pipetting is a critical step when working with ______ specimens.
With a serologic pipette, they may extend to the tip. These pipettes operate on the principle of air displacement. Aspiration in pipetting is a critical step when working with ______ specimens.
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Study Notes
Types of Pipettes
- Volumetric pipettes deliver a single, fixed volume ranging from 0.5 to 200 mL with high accuracy.
- Measuring pipets have a capacity limit of 0.1 to 25 mL, allowing for the delivery of various liquid volumes.
- Glass pipettes are more precise for measurements than plastic bulb pipettes, especially with longer tips.
- Mohr pipettes feature calibration marks only on the stem for volume measurement.
Principles of Operation
- Air displacement is key in the operation of pipettes, allowing for the accurate transfer of liquids.
- Depressing the plunger creates a vacuum in the pipette chamber to draw in liquid.
- Further depressing the plunger releases the liquid into the desired container.
Accuracy and Usage
- Volumetric pipettes are used for accurate solutions preparation, featuring a large bulb with a precise graduation for a specific volume.
- Commonly utilized in analytical chemistry for creating laboratory solutions from base stocks.
- Single-channel and multichannel pipettes differ based on the number of pistons, with multichannel preferred for high-throughput applications.
Advanced Pipetting Tools
- Electronic pipettes enhance ergonomics by minimizing manual force, utilizing a battery-operated motor instead of thumb movement.
- Serologic pipettes may extend to the tip and function on air displacement principles.
- Aspiration is a critical phase in handling liquid specimens during pipetting processes.
Studying That Suits You
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Description
Test your knowledge on pipetting techniques with this quiz prepared by Carl Jethro G.Dellosa, RMT from St. Alexius College Medical Technology Department. The quiz covers topics such as PIPETS permit, transfer of volumes, volumetric pipets, and measuring pipets calibration.
