Podcast
Questions and Answers
What is the primary function of pipettes in a laboratory?
What is the primary function of pipettes in a laboratory?
- To store liquids
- To transfer specific amounts of liquid (correct)
- To analyze chemical composition
- To measure temperature
Which type of pipette is most commonly used in clinical chemistry laboratories?
Which type of pipette is most commonly used in clinical chemistry laboratories?
- Volumetric Pipette
- Serologic Pipette
- Pasteur Pipette
- Automatic pipettes (correct)
What does TC stand for in pipette classification?
What does TC stand for in pipette classification?
- To Contain (correct)
- Time Consistent
- Temperature Controlled
- Total Capacity
What is a defining feature of blowout pipettes?
What is a defining feature of blowout pipettes?
Which of the following is NOT a type of measuring or graduated pipette?
Which of the following is NOT a type of measuring or graduated pipette?
What distinguishes automatic pipettes from other types of pipettes?
What distinguishes automatic pipettes from other types of pipettes?
What should be done with glass pipettes if they are broken?
What should be done with glass pipettes if they are broken?
What volume range do pipettes typically transfer?
What volume range do pipettes typically transfer?
What does TC stand for in the context of pipettes?
What does TC stand for in the context of pipettes?
Which of the following statements about TD pipettes is true?
Which of the following statements about TD pipettes is true?
What is a distinguishing feature of fixed volume micropipettes?
What is a distinguishing feature of fixed volume micropipettes?
Which of the following pipettes would hold a specific volume but not dispense that exact amount?
Which of the following pipettes would hold a specific volume but not dispense that exact amount?
What must be done with a TC pipette before it can accurately deliver the final volume?
What must be done with a TC pipette before it can accurately deliver the final volume?
Which type of pipette generally has a volume capacity of less than 1 mL?
Which type of pipette generally has a volume capacity of less than 1 mL?
Which characteristic separates Class A pipettes from student grade pipettes?
Which characteristic separates Class A pipettes from student grade pipettes?
What is a common characteristic of washout or rinse-out pipettes?
What is a common characteristic of washout or rinse-out pipettes?
What is the primary advantage of using Class A pipettes in critical measurement?
What is the primary advantage of using Class A pipettes in critical measurement?
What does 'To Deliver' (TD) mean in the context of pipettes?
What does 'To Deliver' (TD) mean in the context of pipettes?
Which type of TD pipette has etched rings near the top?
Which type of TD pipette has etched rings near the top?
In the context of pipette drainage, what does non-blown-out mean?
In the context of pipette drainage, what does non-blown-out mean?
What is one of the key specifications to be read on a volumetric pipette?
What is one of the key specifications to be read on a volumetric pipette?
What is a key characteristic of transfer pipettes?
What is a key characteristic of transfer pipettes?
What is the primary purpose of using an Ostwald-Folin pipette?
What is the primary purpose of using an Ostwald-Folin pipette?
What type of pipette is known for being programmable and can dispense into multiple wells simultaneously?
What type of pipette is known for being programmable and can dispense into multiple wells simultaneously?
Which error limit class represents the highest quality in pipettes?
Which error limit class represents the highest quality in pipettes?
What is a common characteristic of air-displacement pipettes?
What is a common characteristic of air-displacement pipettes?
Which pipette type is more accurate than measuring pipets?
Which pipette type is more accurate than measuring pipets?
What should not be used to blow out a pipette's contents?
What should not be used to blow out a pipette's contents?
Which part of the micropipette helps to detach the disposable tip effortlessly?
Which part of the micropipette helps to detach the disposable tip effortlessly?
What is an example of a non-blown-out TD pipette?
What is an example of a non-blown-out TD pipette?
What happens to the liquid when using the Ostwald-Folin pipette?
What happens to the liquid when using the Ostwald-Folin pipette?
What is essential to prevent contamination when using a micropipette?
What is essential to prevent contamination when using a micropipette?
What does the volume adjustment knob on a micropipette do?
What does the volume adjustment knob on a micropipette do?
Which component is absent in P5000 and P10ML types of micropipettes?
Which component is absent in P5000 and P10ML types of micropipettes?
How does a positive-displacement pipette differ from an air-displacement pipette?
How does a positive-displacement pipette differ from an air-displacement pipette?
What is the main function of the sealing end of the shaft in a micropipette?
What is the main function of the sealing end of the shaft in a micropipette?
What is the primary function of the piston in an air-displacement micropipette?
What is the primary function of the piston in an air-displacement micropipette?
What should be done after the plunger is pushed to draw fluid into an air-displacement pipette?
What should be done after the plunger is pushed to draw fluid into an air-displacement pipette?
Which of the following features is indicative of some digital micropipettes?
Which of the following features is indicative of some digital micropipettes?
Why might rinsing and blotting between samples be necessary when using a positive-displacement pipette?
Why might rinsing and blotting between samples be necessary when using a positive-displacement pipette?
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Study Notes
Pipetting Techniques and Classifications
- Pipettes are laboratory tools used to transfer measured amounts of liquid, typically volumes of 20 mL or less.
- Available in reusable (glass) or disposable (plastic) formats, usually open at both ends.
- Larger volumes are generally transferred using automated devices or jar-style pipetting apparatus.
Types of Pipettes
- To Contain (TC): Hold a specific volume but do not deliver that exact volume.
- To Deliver (TD): Dispense the exact amount contained within.
Calibration Marks/Design
- Pipettes are marked with TC or TD designations for user awareness.
- TC pipettes require rinsing out to achieve accurate measurements, while TD pipettes deliver exact volumes.
Drainage Characteristics
- Blown-out (TD): Small amounts remain at the tip and are cleared out.
- Self-draining (Non-blown-out): Drains by gravity, with the complete contents delivered.
Types Based on Function
-
Transfer Pipettes: Designed for transferring liquids; can be either TC or TD.
- Volumetric Pipette: For precise measurement of one specified volume.
- Ostwald-Folin Pipette: For viscous solutions, delivers drop by drop.
- Pasteur Pipette: Commonly used for general transfer tasks.
-
Measuring or Graduated Pipettes: Graduated for multiple volume measurements.
- Serologic Pipette: Used for serum and similar liquids.
- Mohr Pipette: Graduated with a stop for precise measurement.
- Bacteriologic Pipette: For microbiological applications.
Automatic Pipettes
- Designed for convenience and accuracy; widely used in clinical chemistry.
- Micropipettes: Capable of transferring small volumes (10 µL to 1 mL).
- Macropipettes: Transfer volumes greater than 1 mL.
Features of Automatic Pipettes
- Programmable and capable of dispensing in multiple wells simultaneously.
- Key components include:
- Plunger Button: Initiates drawing of liquid.
- Volume Adjustment Knob: Adjusts the aspirated volume.
- Digital Volume Indicator: Displays selected volume.
Types of Automatic Pipettes
- Air-Displacement Pipette: Utilizes suction via a piston, changing disposable tips for each use to avoid contamination.
- Positive-Displacement Pipette: Uses a piston within the tip/barrel, allowing reuse of the same tip, while potentially requiring rinsing between samples.
Important Considerations
- Class A pipettes are recommended for critical measurements due to superior accuracy and precision.
- Familiarity with pipette specifications (class, nominal volume, error limit) is essential for accurate laboratory practice.
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