MLS 11Aa Clinical Chemistry: Pipetting Techniques
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Questions and Answers

What is the primary function of pipettes in a laboratory?

  • To store liquids
  • To transfer specific amounts of liquid (correct)
  • To analyze chemical composition
  • To measure temperature

    • Which type of pipette is most commonly used in clinical chemistry laboratories?

  • Volumetric Pipette
  • Serologic Pipette
  • Pasteur Pipette
  • Automatic pipettes (correct)

    • What does TC stand for in pipette classification?

  • To Contain (correct)
  • Time Consistent
  • Temperature Controlled
  • Total Capacity

    • What is a defining feature of blowout pipettes?

    <p>They drain completely without residue</p> Signup and view all the answers

    Which of the following is NOT a type of measuring or graduated pipette?

    <p>Ball Pipette</p> Signup and view all the answers

    What distinguishes automatic pipettes from other types of pipettes?

    <p>They are operated electronically or are adjustable</p> Signup and view all the answers

    What should be done with glass pipettes if they are broken?

    <p>They can be disposed of</p> Signup and view all the answers

    What volume range do pipettes typically transfer?

    <p>20mL or less</p> Signup and view all the answers

    What does TC stand for in the context of pipettes?

    <p>To Contain</p> Signup and view all the answers

    Which of the following statements about TD pipettes is true?

    <p>TD pipettes dispense the volume indicated on them.</p> Signup and view all the answers

    What is a distinguishing feature of fixed volume micropipettes?

    <p>They are associated with only one specified volume.</p> Signup and view all the answers

    Which of the following pipettes would hold a specific volume but not dispense that exact amount?

    <p>TC pipette</p> Signup and view all the answers

    What must be done with a TC pipette before it can accurately deliver the final volume?

    <p>Fill and rinse it out.</p> Signup and view all the answers

    Which type of pipette generally has a volume capacity of less than 1 mL?

    <p>Micropipette</p> Signup and view all the answers

    Which characteristic separates Class A pipettes from student grade pipettes?

    <p>Accuracy and precision</p> Signup and view all the answers

    What is a common characteristic of washout or rinse-out pipettes?

    <p>They must be rinsed before reusing for accurate measurements.</p> Signup and view all the answers

    What is the primary advantage of using Class A pipettes in critical measurement?

    <p>They maximize accuracy and precision.</p> Signup and view all the answers

    What does 'To Deliver' (TD) mean in the context of pipettes?

    <p>The pipette delivers a specified volume of liquid.</p> Signup and view all the answers

    Which type of TD pipette has etched rings near the top?

    <p>Blown-out pipet</p> Signup and view all the answers

    In the context of pipette drainage, what does non-blown-out mean?

    <p>Liquid is allowed to drain by gravity.</p> Signup and view all the answers

    What is one of the key specifications to be read on a volumetric pipette?

    <p>Brand</p> Signup and view all the answers

    What is a key characteristic of transfer pipettes?

    <p>They are available in blown-out and non-blown-out types.</p> Signup and view all the answers

    What is the primary purpose of using an Ostwald-Folin pipette?

    <p>For measuring viscous solutions such as blood</p> Signup and view all the answers

    What type of pipette is known for being programmable and can dispense into multiple wells simultaneously?

    <p>Automatic Macropipette</p> Signup and view all the answers

    Which error limit class represents the highest quality in pipettes?

    <p>Class A</p> Signup and view all the answers

    What is a common characteristic of air-displacement pipettes?

    <p>They utilize a mechanical mechanism to aspirate liquid</p> Signup and view all the answers

    Which pipette type is more accurate than measuring pipets?

    <p>Transfer pipette</p> Signup and view all the answers

    What should not be used to blow out a pipette's contents?

    <p>Mouth</p> Signup and view all the answers

    Which part of the micropipette helps to detach the disposable tip effortlessly?

    <p>Tip Ejector Button</p> Signup and view all the answers

    What is an example of a non-blown-out TD pipette?

    <p>Volumetric pipette</p> Signup and view all the answers

    What happens to the liquid when using the Ostwald-Folin pipette?

    <p>It is blown out completely</p> Signup and view all the answers

    What is essential to prevent contamination when using a micropipette?

    <p>Changing the tip after each use</p> Signup and view all the answers

    What does the volume adjustment knob on a micropipette do?

    <p>Adjusts the volume to be aspirated</p> Signup and view all the answers

    Which component is absent in P5000 and P10ML types of micropipettes?

    <p>Tip ejector button</p> Signup and view all the answers

    How does a positive-displacement pipette differ from an air-displacement pipette?

    <p>It moves the piston in the pipette tip or barrel</p> Signup and view all the answers

    What is the main function of the sealing end of the shaft in a micropipette?

    <p>To secure the disposable tip</p> Signup and view all the answers

    What is the primary function of the piston in an air-displacement micropipette?

    <p>To create suction that draws a sample into the tip</p> Signup and view all the answers

    What should be done after the plunger is pushed to draw fluid into an air-displacement pipette?

    <p>The tip must be placed against the sample before releasing the plunger</p> Signup and view all the answers

    Which of the following features is indicative of some digital micropipettes?

    <p>Visual display of the volume</p> Signup and view all the answers

    Why might rinsing and blotting between samples be necessary when using a positive-displacement pipette?

    <p>To prevent cross-contamination between samples</p> Signup and view all the answers

    Study Notes

    Pipetting Techniques and Classifications

    • Pipettes are laboratory tools used to transfer measured amounts of liquid, typically volumes of 20 mL or less.
    • Available in reusable (glass) or disposable (plastic) formats, usually open at both ends.
    • Larger volumes are generally transferred using automated devices or jar-style pipetting apparatus.

    Types of Pipettes

    • To Contain (TC): Hold a specific volume but do not deliver that exact volume.
    • To Deliver (TD): Dispense the exact amount contained within.

    Calibration Marks/Design

    • Pipettes are marked with TC or TD designations for user awareness.
    • TC pipettes require rinsing out to achieve accurate measurements, while TD pipettes deliver exact volumes.

    Drainage Characteristics

    • Blown-out (TD): Small amounts remain at the tip and are cleared out.
    • Self-draining (Non-blown-out): Drains by gravity, with the complete contents delivered.

    Types Based on Function

    • Transfer Pipettes: Designed for transferring liquids; can be either TC or TD.

      • Volumetric Pipette: For precise measurement of one specified volume.
      • Ostwald-Folin Pipette: For viscous solutions, delivers drop by drop.
      • Pasteur Pipette: Commonly used for general transfer tasks.

    • Measuring or Graduated Pipettes: Graduated for multiple volume measurements.

      • Serologic Pipette: Used for serum and similar liquids.
      • Mohr Pipette: Graduated with a stop for precise measurement.
      • Bacteriologic Pipette: For microbiological applications.

    Automatic Pipettes

    • Designed for convenience and accuracy; widely used in clinical chemistry.
    • Micropipettes: Capable of transferring small volumes (10 µL to 1 mL).
    • Macropipettes: Transfer volumes greater than 1 mL.

    Features of Automatic Pipettes

    • Programmable and capable of dispensing in multiple wells simultaneously.
    • Key components include:
      • Plunger Button: Initiates drawing of liquid.
      • Volume Adjustment Knob: Adjusts the aspirated volume.
      • Digital Volume Indicator: Displays selected volume.

    Types of Automatic Pipettes

    • Air-Displacement Pipette: Utilizes suction via a piston, changing disposable tips for each use to avoid contamination.
    • Positive-Displacement Pipette: Uses a piston within the tip/barrel, allowing reuse of the same tip, while potentially requiring rinsing between samples.

    Important Considerations

    • Class A pipettes are recommended for critical measurements due to superior accuracy and precision.
    • Familiarity with pipette specifications (class, nominal volume, error limit) is essential for accurate laboratory practice.

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    MLS 11Aa Clinical Chemistry Pipetting Techniques PDF

    Description

    Test your knowledge on pipetting techniques and classifications in clinical chemistry. This quiz covers the different types of pipettes, their calibration marks, and the importance of accuracy in lab environments. Perfect for MLS students and professionals looking to refine their skills.

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