Podcast
Questions and Answers
Why is accurate pipetting crucial in molecular projects?
Why is accurate pipetting crucial in molecular projects?
- To ensure the success and reproducibility of experiments (correct)
- To make the experiments more expensive
- To prevent damage to the pipettes
- To avoid contamination in PCRs
What should never be done to a pipette with a filled tip?
What should never be done to a pipette with a filled tip?
- Adjust the volume beyond the maximum setting
- Rotate the volume adjustor beyond the upper limit
- Use it without a tip
- Lay it down or turn it upside down (correct)
What happens if the plunger is allowed to snap back after withdrawing or ejecting fluid?
What happens if the plunger is allowed to snap back after withdrawing or ejecting fluid?
- It damages the piston (correct)
- It contaminates the liquid
- It causes calibration changes
- It results in high expense
What should never be done when using a pipette?
What should never be done when using a pipette?
What indicates the decimal point on the volume adjustment of a pipette?
What indicates the decimal point on the volume adjustment of a pipette?
What is the easiest means of determining DNA concentration?
What is the easiest means of determining DNA concentration?
At what concentration does dissolved DNA become viscous?
At what concentration does dissolved DNA become viscous?
What is the relationship between absorbance and DNA concentration?
What is the relationship between absorbance and DNA concentration?
Which formula is used to determine the DNA concentration of a solution?
Which formula is used to determine the DNA concentration of a solution?
What should be added to the 'D' tube during concentration determination using the spectrophotometer?
What should be added to the 'D' tube during concentration determination using the spectrophotometer?
What is the relationship between DNA concentration and UV light absorption?
What is the relationship between DNA concentration and UV light absorption?
What happens to dissolved DNA at high concentrations (10 mg/ml and above)?
What happens to dissolved DNA at high concentrations (10 mg/ml and above)?
What should be added to the 'B' tube during concentration determination using the spectrophotometer?
What should be added to the 'B' tube during concentration determination using the spectrophotometer?
What indicates the decimal point on the volume adjustment of a pipette?
What indicates the decimal point on the volume adjustment of a pipette?
Why is accurate pipetting crucial in molecular projects?
Why is accurate pipetting crucial in molecular projects?
