Pipetting Proficiency in Molecular Projects

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Why is accurate pipetting crucial in molecular projects?

To ensure the success and reproducibility of experiments (correct)

To make the experiments more expensive

To prevent damage to the pipettes

To avoid contamination in PCRs

What should never be done to a pipette with a filled tip?

Adjust the volume beyond the maximum setting

Rotate the volume adjustor beyond the upper limit

Use it without a tip

Lay it down or turn it upside down (correct)

What happens if the plunger is allowed to snap back after withdrawing or ejecting fluid?

It damages the piston (correct)

It contaminates the liquid

It causes calibration changes

It results in high expense

What should never be done when using a pipette?

Adjust the volume beyond the maximum setting Signup and view all the answers

What indicates the decimal point on the volume adjustment of a pipette?

Red/black color Signup and view all the answers

What is the easiest means of determining DNA concentration?

Through spectrophotometric analysis Signup and view all the answers

At what concentration does dissolved DNA become viscous?

10 mg/ml Signup and view all the answers

What is the relationship between absorbance and DNA concentration?

Linear relationship Signup and view all the answers

Which formula is used to determine the DNA concentration of a solution?

$25 Unknown µg/ml = 50 µg/ml x Measured A260 x dilution factor$ Signup and view all the answers

What should be added to the 'D' tube during concentration determination using the spectrophotometer?

1.99 ml of TE buffer Signup and view all the answers

What is the relationship between DNA concentration and UV light absorption?

The higher the DNA concentration, the more UV light it will absorb Signup and view all the answers

What happens to dissolved DNA at high concentrations (10 mg/ml and above)?

It becomes more viscous Signup and view all the answers

Pipetting Proficiency in Molecular Projects

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Questions and Answers

Why is accurate pipetting crucial in molecular projects?

What should never be done to a pipette with a filled tip?

What happens if the plunger is allowed to snap back after withdrawing or ejecting fluid?

What should never be done when using a pipette?

What indicates the decimal point on the volume adjustment of a pipette?

What is the easiest means of determining DNA concentration?

At what concentration does dissolved DNA become viscous?

What is the relationship between absorbance and DNA concentration?

Which formula is used to determine the DNA concentration of a solution?

What should be added to the 'D' tube during concentration determination using the spectrophotometer?

What is the relationship between DNA concentration and UV light absorption?

What happens to dissolved DNA at high concentrations (10 mg/ml and above)?

What should be added to the 'B' tube during concentration determination using the spectrophotometer?

What indicates the decimal point on the volume adjustment of a pipette?

Why is accurate pipetting crucial in molecular projects?

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