Pipetting Proficiency in Molecular Projects
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Questions and Answers

Why is accurate pipetting crucial in molecular projects?

  • To ensure the success and reproducibility of experiments (correct)
  • To make the experiments more expensive
  • To prevent damage to the pipettes
  • To avoid contamination in PCRs

    • What should never be done to a pipette with a filled tip?

  • Adjust the volume beyond the maximum setting
  • Rotate the volume adjustor beyond the upper limit
  • Use it without a tip
  • Lay it down or turn it upside down (correct)

    • What happens if the plunger is allowed to snap back after withdrawing or ejecting fluid?

  • It damages the piston (correct)
  • It contaminates the liquid
  • It causes calibration changes
  • It results in high expense

    • What should never be done when using a pipette?

    <p>Adjust the volume beyond the maximum setting</p> Signup and view all the answers

    What indicates the decimal point on the volume adjustment of a pipette?

    <p>Red/black color</p> Signup and view all the answers

    What is the easiest means of determining DNA concentration?

    <p>Through spectrophotometric analysis</p> Signup and view all the answers

    At what concentration does dissolved DNA become viscous?

    <p>10 mg/ml</p> Signup and view all the answers

    What is the relationship between absorbance and DNA concentration?

    <p>Linear relationship</p> Signup and view all the answers

    Which formula is used to determine the DNA concentration of a solution?

    <p>$25 Unknown µg/ml = 50 µg/ml x Measured A260 x dilution factor$</p> Signup and view all the answers

    What should be added to the 'D' tube during concentration determination using the spectrophotometer?

    <p>1.99 ml of TE buffer</p> Signup and view all the answers

    What is the relationship between DNA concentration and UV light absorption?

    <p>The higher the DNA concentration, the more UV light it will absorb</p> Signup and view all the answers

    What happens to dissolved DNA at high concentrations (10 mg/ml and above)?

    <p>It becomes more viscous</p> Signup and view all the answers

    What should be added to the 'B' tube during concentration determination using the spectrophotometer?

    <p>2 ml of TE buffer</p> Signup and view all the answers

    What indicates the decimal point on the volume adjustment of a pipette?

    <p>A small line on the pipette barrel</p> Signup and view all the answers

    Why is accurate pipetting crucial in molecular projects?

    <p>To minimize contamination</p> Signup and view all the answers

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