Photosynthesis Light Independent Stage
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Questions and Answers

What is the primary function of ATP and reduced NADP in the photosynthesis process?

  • To reduce carbon dioxide (correct)
  • To absorb light energy
  • To regenerate ribulose bisphosphate
  • To form six-carbon sugars
  • Where does the light independent stage of photosynthesis take place?

  • In the stroma of chloroplasts (correct)
  • In the cytoplasm
  • In the thylakoid membranes
  • In the chloroplast envelope
  • What role does the enzyme ribulose bisphosphate carboxylase (rubisco) play in the Calvin cycle?

  • It catalyzes the fixation of carbon dioxide (correct)
  • It converts starch to sugars
  • It regenerates ribulose bisphosphate
  • It synthesizes ATP
  • What is formed when the unstable six-carbon compound in the Calvin cycle breaks down?

    <p>Two molecules of glycerate 3-phosphate</p> Signup and view all the answers

    How many triose phosphate molecules are used to regenerate ribulose bisphosphate in the Calvin cycle?

    <p>Five out of every six</p> Signup and view all the answers

    What is the main product of pairing two triose phosphate molecules in the Calvin cycle?

    <p>Hexose sugars</p> Signup and view all the answers

    Which process is primarily responsible for the synthesis of carbohydrates in plants?

    <p>Calvin cycle</p> Signup and view all the answers

    What is the initial molecule that carbon dioxide combines with in the Calvin cycle?

    <p>Ribulose bisphosphate</p> Signup and view all the answers

    What happens when light is absent concerning the light independent stage of photosynthesis?

    <p>It ceases rapidly</p> Signup and view all the answers

    What is the significance of the Calvin cycle in the context of plant metabolism?

    <p>It synthesizes organic molecules from inorganic compounds</p> Signup and view all the answers

    What happens to the majority of triose phosphate molecules produced in the Calvin cycle?

    <p>They are used to regenerate ribulose bisphosphate.</p> Signup and view all the answers

    What is the role of ATP in the Calvin cycle?

    <p>It is used to convert glycerate 3-phosphate to triose phosphate.</p> Signup and view all the answers

    During the Calvin cycle, what is the immediate product formed when carbon dioxide combines with ribulose bisphosphate?

    <p>Unstable six-carbon compound.</p> Signup and view all the answers

    How are hydrogen ions provided for the reduction reaction in the Calvin cycle?

    <p>They come from reduced NADP formed during the light dependent stage.</p> Signup and view all the answers

    What occurs in the stroma of chloroplasts during the light independent stage?

    <p>The Calvin cycle processes carbon dioxide into sugars.</p> Signup and view all the answers

    How does water contribute to temperature stability in aquatic environments?

    <p>It has a high specific heat capacity, enabling slow temperature changes.</p> Signup and view all the answers

    What is the primary reason ice floats on liquid water?

    <p>The molecular structure of ice is less dense than that of liquid water.</p> Signup and view all the answers

    Which property of water is responsible for its high surface tension?

    <p>Hydrogen bonds between water molecules.</p> Signup and view all the answers

    Which characteristic of water helps in the transport of molecules in living organisms?

    <p>Water's ability to dissolve polar substances.</p> Signup and view all the answers

    Why does water have high melting and boiling points compared to other substances?

    <p>Hydrogen bonds require a significant amount of energy to break.</p> Signup and view all the answers

    How does the process of cohesion contribute to the properties of water?

    <p>It enables water molecules to form a meniscus in tubes.</p> Signup and view all the answers

    What role does water's transparency play in aquatic ecosystems?

    <p>It allows sunlight to penetrate for photosynthesis.</p> Signup and view all the answers

    What is a consequence of ice floating on water for aquatic organisms?

    <p>It insulates the water beneath, reducing freezing risks for organisms.</p> Signup and view all the answers

    What defines water as an excellent solvent in biological systems?

    <p>It can interact with ions and polar molecules through hydrogen bonds.</p> Signup and view all the answers

    What causes the polarity of water molecules?

    <p>The unequal sharing of electrons between hydrogen and oxygen.</p> Signup and view all the answers

    How do water molecules demonstrate high surface tension?

    <p>Due to the strong hydrogen bonds between water molecules.</p> Signup and view all the answers

    What is one significant role of water as a solvent in biological systems?

    <p>It allows for metabolic reactions by dissolving reactants.</p> Signup and view all the answers

    Why does water have a high specific heat capacity?

    <p>Due to the large number of hydrogen bonds between water molecules.</p> Signup and view all the answers

    In which way does water facilitate transport in plants?

    <p>By dissolving and transporting nutrients through xylem vessels.</p> Signup and view all the answers

    What factor contributes to the high melting and boiling points of water?

    <p>The energy required to break the hydrogen bonds.</p> Signup and view all the answers

    What happens to the charge of water molecules?

    <p>Oxygen becomes slightly negatively charged while hydrogen is slightly positively charged.</p> Signup and view all the answers

    How does the dipolar nature of water affect ionic substances?

    <p>It allows them to dissolve by separating ions.</p> Signup and view all the answers

    What role does water play in maintaining cell membrane stability?

    <p>It maintains appropriate osmotic balance.</p> Signup and view all the answers

    What characteristic enables water to be an excellent solvent for polar molecules?

    <p>Its dipolar nature.</p> Signup and view all the answers

    Study Notes

    Light Independent Stage of Photosynthesis

    • ATP and reduced NADP (NADPH H+) produced in the light dependent stage are essential for the light independent stage, also known as the Calvin cycle.
    • This stage does not require light directly but relies on products from the light dependent stage, halting when light is absent.
    • Occurs in the stroma of chloroplasts and was described by Melvin Calvin and his team.
    • The Calvin cycle consists of several enzyme-controlled stages.

    Stages of the Calvin Cycle

    • Carbon dioxide enters the leaf through stomata, dissolves in surrounding water, and diffuses into the stroma of chloroplasts.
    • In the stroma, carbon dioxide reacts with ribulose bisphosphate (RuBP) using the enzyme ribulose bisphosphate carboxylase (rubisco), forming an unstable six-carbon compound.
    • This six-carbon compound splits into two molecules of glycerate 3-phosphate (GP), a three-carbon compound.
    • One ATP molecule from the light dependent reaction converts GP into triose phosphate (TP), also a three-carbon sugar.
    • Reduced NADP contributes hydrogen to reduce GP to TP.
    • Triose phosphate molecules pair up to form six-carbon (hexose) sugars.
    • Six-carbon sugars can polymerize to create starch, a storage carbohydrate.
    • Five out of every six TP molecules regenerate RuBP using remaining ATP, ensuring the cycle continues.

    Synthesis of Organic Compounds

    • Plants synthesize various organic molecules, including carbohydrates, lipids, and proteins, from Calvin cycle compounds.
    • Carbohydrates like sucrose are created by combining glucose and fructose; glucose serves as a respiratory substrate.
    • Starch (storage carbohydrate) and cellulose (cell wall component) are formed by polymerizing glucose differently.
    • Lipids consist of glycerol and fatty acids derived from triose phosphate and glycerate 3-phosphate, respectively.
    • Lipids serve for storage and structural functions in cell membranes.
    • Proteins, essential for cell structure and enzymatic functions, are composed of amino acids produced from glycerate 3-phosphate through acetyl coenzyme A and Krebs cycle intermediates.

    Light Independent Stage of Photosynthesis

    • ATP and reduced NADP (NADPH H+) produced in the light dependent stage are essential for the light independent stage, also known as the Calvin cycle.
    • This stage does not require light directly but relies on products from the light dependent stage, halting when light is absent.
    • Occurs in the stroma of chloroplasts and was described by Melvin Calvin and his team.
    • The Calvin cycle consists of several enzyme-controlled stages.

    Stages of the Calvin Cycle

    • Carbon dioxide enters the leaf through stomata, dissolves in surrounding water, and diffuses into the stroma of chloroplasts.
    • In the stroma, carbon dioxide reacts with ribulose bisphosphate (RuBP) using the enzyme ribulose bisphosphate carboxylase (rubisco), forming an unstable six-carbon compound.
    • This six-carbon compound splits into two molecules of glycerate 3-phosphate (GP), a three-carbon compound.
    • One ATP molecule from the light dependent reaction converts GP into triose phosphate (TP), also a three-carbon sugar.
    • Reduced NADP contributes hydrogen to reduce GP to TP.
    • Triose phosphate molecules pair up to form six-carbon (hexose) sugars.
    • Six-carbon sugars can polymerize to create starch, a storage carbohydrate.
    • Five out of every six TP molecules regenerate RuBP using remaining ATP, ensuring the cycle continues.

    Synthesis of Organic Compounds

    • Plants synthesize various organic molecules, including carbohydrates, lipids, and proteins, from Calvin cycle compounds.
    • Carbohydrates like sucrose are created by combining glucose and fructose; glucose serves as a respiratory substrate.
    • Starch (storage carbohydrate) and cellulose (cell wall component) are formed by polymerizing glucose differently.
    • Lipids consist of glycerol and fatty acids derived from triose phosphate and glycerate 3-phosphate, respectively.
    • Lipids serve for storage and structural functions in cell membranes.
    • Proteins, essential for cell structure and enzymatic functions, are composed of amino acids produced from glycerate 3-phosphate through acetyl coenzyme A and Krebs cycle intermediates.

    General Features of Water

    • Water is a dipolar molecule with an oxygen atom carrying a slight negative charge (Ƨ -) and hydrogen atoms carrying slight positive charges (Ƨ +).
    • Polarity leads to the formation of hydrogen bonds, where water molecules are attracted to one another.
    • Oxygen is more electronegative than hydrogen, causing uneven electron sharing; this results in the partial charges in the molecule.
    • As an excellent solvent, water enables the transport of polar molecules and ions, dissolving substances like oxygen, carbon dioxide, amino acids, and glucose.
    • Water has high melting and boiling points due to the significant energy required to break hydrogen bonds connecting the molecules.
    • High surface tension arises from stronger cohesion between water molecules compared to their adhesion with air, forming a 'skin' on the surface.

    Properties of Water

    • Acts as an excellent solvent for ions and polar molecules, facilitating biochemical reactions in living cells by allowing reactants to dissolve and move freely.
    • Maintains the stability of cell membranes, essential for cellular function.
    • Functions as a transport medium:
      • In animals, water carries digested food, hormones, antibodies, and waste in blood and lymphatic systems.
      • In plants, water transports salts through xylem and sugars in phloem.

    High Specific Heat Capacity

    • Water has a high specific heat capacity, requiring 4200 joules to raise 1 kg of water by 1°C.
    • This property enables water to resist temperature changes, stabilizing environments:
      • Lakes and oceans experience slow temperature change, providing a stable habitat for aquatic life.
      • Maintains constant temperatures in the bodies of living organisms, essential for biochemical reactions and optimal enzymatic activity.

    Density and Freezing Point

    • Ice floats on liquid water, creating an insulating layer, which protects aquatic life from freezing conditions.
    • Low-density ice reduces the risk of aquatic organisms freezing completely during cold temperatures.

    Cohesion and Surface Tension

    • Cohesion refers to water molecules sticking together due to hydrogen bonds, contributing to water's overall cohesion.
    • Adhesion is the attraction between water molecules and different substances, enhancing water interaction with the environment.
    • High surface tension minimizes surface area due to stronger hydrogen bond attractions among water molecules compared to the attraction to air.

    Implications for Aquatic Life

    • Transparency of water allows sufficient light penetration for photosynthesis in aquatic organisms.
    • Insulating properties from floating ice help maintain underlying water temperatures.
    • High specific heat capacity makes water more resistant to temperature fluctuations, benefiting the functioning of enzymes in aquatic environments.

    Transport of Molecules

    • Water’s polar nature enables it to dissolve ions and polar compounds effectively.
    • The positive hydrogen ends attract negatively charged ions (anions), while the negative oxygen end attracts positively charged ions (cations), facilitating nutrient transport in organisms.

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    Explore the light independent stage of photosynthesis, where ATP and NADPH are utilized to reduce carbon dioxide. Learn how this stage, occurring in the stroma of chloroplasts, is essential yet does not rely on direct light. Discover the impact of light absence on this crucial process.

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