BIOC 4520: Metabolic Process Lec 7
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Questions and Answers

what is the initial Stable Product of CO₂ fixation in Photosynthesis?

3-phosphoglycerate (3-PGA).

what is the acceptor Molecule for CO₂?

RuBP

How Is the Acceptor (RuBP) Synthesized?

RuBP is synthesized from glyceraldehyde-3-phosphate (G3P)

Path of Carbon to Sugar (Specifically Sucrose, Not Glucose)?

<p>After CO₂ is fixed and reduced to form G3P, some of it can be synthesized to form sugars. in the cytosol, G3P can combine with stuff to form sucrose</p> Signup and view all the answers

What was the primary purpose of using carbon-14 (¹⁴C) in Calvin's experiments?

<p>To trace the pathway of CO₂ fixation in photosynthesis.</p> Signup and view all the answers

Which technique was used by Calvin and his team to separate intermediates of the Calvin cycle?

<p>2D paper chromatography</p> Signup and view all the answers

What was the first stable compound identified in Calvin's carbon-14 experiments?

<p>3-PGA</p> Signup and view all the answers

Which of the following is NOT a direct product of the Calvin cycle as revealed by Calvin's ¹⁴C experiments?

<p>sucrose</p> Signup and view all the answers

What is the role of UDP-glucose (UPDG) in carbon metabolism?

<p>C) It is involved in the biosynthesis of sucrose.</p> Signup and view all the answers

In the carbon-14 pulse-chase experiment, what is the main purpose of the pulse phase?

<p>To introduce radioactive carbon into the products of carbon fixation</p> Signup and view all the answers

In the chase phase of a carbon-14 pulse-chase experiment, what happens to the radioactive carbon-14 label?

<p>It is incorporated into successive intermediates of the Calvin cycle.</p> Signup and view all the answers

Which enzyme catalyzes the CO₂ fixation step in the Calvin cycle, where ribulose-1,5-bisphosphate reacts with CO₂?

<p>rubisco</p> Signup and view all the answers

In the Calvin cycle, what is the product of CO₂ fixation involving 3 molecules of ribulose-1,5-bisphosphate (RuBP) and 3 molecules of CO₂?

<p>6 molecules of 3-PGA</p> Signup and view all the answers

During the reduction stage of the Calvin cycle, (3-PGA) is REDUCED to glyceraldehyde-3-phosphate (G3P) using, which molecules and which enzyme

<p>6 NADPH and GAP dehydrogenase</p> Signup and view all the answers

when 3-PGA is phosphorylated what is used

<p>6 ATP and 3-PGA kinase</p> Signup and view all the answers

What is the total number of ATP molecules required to complete one turn of the Calvin cycle and regenerate (RuBP)?

<p>9</p> Signup and view all the answers

What are the overall products of the Calvin cycle when 3 molecules of CO₂ are fixed?

<p>1 glyceraldehyde-3-phosphate (G3P), 6 NADP⁺, 9 ADP, 8 Pi , 3H20</p> Signup and view all the answers

Which of the following stages in the Calvin cycle requires additional ATP for the process to proceed?

<p>Regeneration of ribulose-1,5-bisphosphate (RuBP)</p> Signup and view all the answers

where does calvin cycle occur

<p>occurs in the stroma of the chloroplasts.</p> Signup and view all the answers

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