Photosynthesis and Bacterial Energetics Quiz

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Questions and Answers

What are the two main functions of photosynthesis?

  • ATP generation and NAD(P)H production (correct)
  • Light absorption and energy storage
  • Producing ATP and creating glucose
  • Water splitting and oxygen release

What role do accessory pigments like carotenoids play in photosynthesis?

  • They generate ATP directly through photophosphorylation.
  • They replace chlorophyll in oxygenic photosynthesis.
  • They assist in broader wavelength access for light absorption. (correct)
  • They are the primary light-capturing pigments.

Which molecule is primarily responsible for light absorption in photosynthesis?

  • Chlorophyll b
  • Carotene
  • Chlorophyll a (correct)
  • Bacteriophyll

What is the significance of CO2 fixation in photosynthesis?

<p>It converts CO2 back into organic matter. (C)</p> Signup and view all the answers

What is the primary energy source for the electron transport chain in photosynthesis?

<p>Light energy (C)</p> Signup and view all the answers

Which component is essential for generating ATP through photosynthesis in starved bacterial cells?

<p>Proteorhodopsin (C)</p> Signup and view all the answers

What happens to chlorophyll pigments during fall, contributing to visible colors?

<p>Chlorophyll is degraded, revealing accessory pigments. (C)</p> Signup and view all the answers

Which of the following statements about anoxygenic phototrophs is correct?

<p>They utilize alternative electron donors that are not water. (C)</p> Signup and view all the answers

What ability allows Salmonella typhimurium to outcompete other bacteria?

<p>Using tetrathionate as a terminal electron acceptor (C)</p> Signup and view all the answers

How do lactic acid bacteria (LAB) produce ATP?

<p>By substrate level phosphorylation during glycolysis (B)</p> Signup and view all the answers

In the context of bioenergetics, what role does ATP synthase play?

<p>It acts as an ATP-driven proton pump in some conditions (B)</p> Signup and view all the answers

Which pigment is NOT commonly found in cyanobacteria?

<p>Chlorophyll b (B)</p> Signup and view all the answers

What happens to electrons released during glucose oxidation?

<p>They flow through the electron transport chain for ATP production (D)</p> Signup and view all the answers

What is a product of the TCA cycle that is critical for energy production in cells?

<p>FADH (C)</p> Signup and view all the answers

What is the primary ecological role of Prochlorococcus?

<p>Dominant primary producer at mid latitudes (C)</p> Signup and view all the answers

What unique habitat can desert crust cyanobacteria thrive in?

<p>Harsh desert environments (D)</p> Signup and view all the answers

Which molecule is not a primary electron acceptor in aerobic respiration?

<p>Tetrathionate (D)</p> Signup and view all the answers

What does the term 'substrate level phosphorylation' refer to?

<p>ATP synthesis from inorganic phosphate and ADP without a proton gradient (D)</p> Signup and view all the answers

Which component is essential for the non-cyclic electron flow in photosystems?

<p>Mn4Ca site (D)</p> Signup and view all the answers

What characteristic of cyanobacteria allows them to be effective in oxygenic photosynthesis?

<p>Multiple accessory pigments (A)</p> Signup and view all the answers

What is the final product of glucose metabolism through fermentation?

<p>Lactic acid or ethanol (A)</p> Signup and view all the answers

Which of the following statements about microbial mats is true?

<p>They can consist of various groups of microorganisms. (A)</p> Signup and view all the answers

What depth range does Prochlorococcus typically inhabit?

<p>20-200 m (C)</p> Signup and view all the answers

What is the role of phycobilins in cyanobacteria?

<p>Absorb light for photosynthesis (D)</p> Signup and view all the answers

What is the primary function of chlorosomes in green sulfur bacteria?

<p>They improve photosynthetic efficiency under low light. (B)</p> Signup and view all the answers

Which electron donor is favored by green sulfur bacteria for photosynthesis?

<p>Hydrogen sulfide (H2S) (D)</p> Signup and view all the answers

What distinguishes the CO2 fixation pathway in green sulfur bacteria from the RPP cycle?

<p>It is based on a reverse tricarboxylic acid cycle. (A)</p> Signup and view all the answers

How do green sulfur bacteria manage to survive in environments with low light?

<p>By developing chlorosomes. (C)</p> Signup and view all the answers

Which of the following statements about the electron donor crisis is true?

<p>Anoxygenic photosynthetic bacteria were limited by available electron donors. (A)</p> Signup and view all the answers

In green sulfur bacteria, where do electrons flow after being released from the FeS protein?

<p>To ferredoxin (Fd) (A)</p> Signup and view all the answers

Which of the following organisms were the first to fix CO2 using a pathway different from the RPP cycle?

<p>Green sulfur bacteria (C)</p> Signup and view all the answers

What is a key difference between the electron flow in oxygenic photosynthesis and that in green sulfur bacteria?

<p>Oxygenic photosynthesis uses water as an electron donor. (C)</p> Signup and view all the answers

What is the primary role of photons in photosynthesis?

<p>To provide energy to eject electrons from chlorophyll. (D)</p> Signup and view all the answers

How does the reductive pentose phosphate (RPP) cycle function in purple bacteria?

<p>It relies on organic electron donors for carbon fixation. (D)</p> Signup and view all the answers

Which of the following is true about the electron donors in oxygenic and anoxygenic photosynthesis?

<p>Water is an unfavorable electron donor for CO2 fixation. (C)</p> Signup and view all the answers

What distinguishes purple sulfur bacteria from purple nonsulfur bacteria?

<p>Production of granules from internal S0. (B)</p> Signup and view all the answers

What is the significance of carotenoids in purple bacteria?

<p>They absorb light energy during photosynthesis. (C)</p> Signup and view all the answers

Which phylum includes both purple sulfur bacteria and purple nonsulfur bacteria?

<p>Proteobacteria (B)</p> Signup and view all the answers

In comparison to H2O, how does H2S function as an electron donor in CO2 fixation?

<p>H2S is a superior electron donor for CO2 fixation. (C)</p> Signup and view all the answers

What role does the CCCP play in bacterial metabolism?

<p>It acts as an uncoupler that disrupts the proton gradient. (A)</p> Signup and view all the answers

What is the expected output from the RPP cycle when fixing 6 CO2 molecules?

<p>1 glucose molecule and 18 ATP. (D)</p> Signup and view all the answers

Which of the following statements about energy requirements for photosynthesis is correct?

<p>Anoxygenic processes use less energy due to inefficient electron donors. (A)</p> Signup and view all the answers

Flashcards

Salmonella typhimurium

A type of bacteria that can use tetrathionate as a terminal electron acceptor. This allows Salmonella to outcompete other bacteria in the gut during an inflammatory response.

Respiratory chain using tetrathionate

A process where a bacterium uses waste products, like tetrathionate, as a final electron acceptor in its respiratory chain. This allows the bacterium to generate energy.

Lactic acid bacteria (LAB)

A type of bacteria that produces lactic acid as a byproduct of fermentation. They generate ATP through substrate-level phosphorylation during glycolysis.

F1Fo ATPase

The enzyme complex that generates ATP through oxidative phosphorylation. Its function is reversible, meaning it can either pump protons across the membrane or generate ATP, depending on the conditions.

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Electron transport chain

The movement of electrons along a chain of proteins, coupled with the pumping of protons across a membrane. This process generates a proton motive force that drives ATP synthesis.

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Oxidative phosphorylation

A process where electrons are transferred from a donor molecule, like glucose, to a final acceptor molecule, like oxygen. This process releases energy used for ATP synthesis.

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Glucose to CO2 overview

The breakdown of glucose into pyruvate and then into acetyl-CoA, followed by the citric acid cycle. This process produces electrons carried by NADH and FADH2, which are then used in the electron transport chain for ATP synthesis.

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Photosynthesis

The process by which plants capture light energy and convert it into chemical energy in the form of glucose. It is the foundation of life on Earth.

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Bacteriorhodopsin

A type of protein that uses light energy to pump protons across a membrane, creating a proton gradient that drives ATP synthesis.

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The two main functions of photosynthesis

Photosynthesis is the process by which organisms convert light energy into chemical energy in the form of ATP and NAD(P)H. The process is essentially a light-driven redox reaction and the light energy can be used to move electrons from a low-energy to a high-energy state to create a gradient that produces ATP.

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Chlorophyll a

Chlorophyll a is a pigment that absorbs light energy, primarily in the red and blue wavelengths, and uses it to power the light-dependent reactions of photosynthesis.

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Bacteriochlorophyll a

Bacteriochlorophyll a is similar to chlorophyll a, but absorbs light in the infrared region of the spectrum. It is found in some photosynthetic bacteria.

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Accessory pigments

Accessory pigments help expand the range of light wavelengths a photosynthetic organism can use. Carotenoids are an important example, absorbing light energy from the green wavelengths that chlorophyll a does not absorb well.

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Carotenoids

Carotenoids are a group of pigments that are important for light harvesting in photosynthesis. They absorb light in the blue and green wavelengths, giving plants their orange and yellow hues.

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Fall colors

The degradation of chlorophyll in autumn leaves reveals other pigments, such as carotenoids and anthocyanins, creating the vibrant fall colors.

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Exotic electron donors

Photosynthetic organisms utilize electron donors that are not chemically favorable on their own.  The light energy is harnessed to move electrons to higher energy states and create a favorable condition for energy production. 

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Oxygenic Photosynthesis

Photosynthesis which uses water as the source of electrons and produces oxygen as a byproduct.

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Anoxygenic Photosynthesis

Photosynthesis which uses other molecules like hydrogen sulfide as the source of electrons and does not produce oxygen.

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Cyanobacteria

A type of photosynthetic bacteria that uses water as the electron donor and releases oxygen as a byproduct.

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Purple bacteria

A group of photosynthetic bacteria that use various electron donors and DO NOT produce oxygen.

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Purple sulfur bacteria

Purple bacteria that use reduced sulfur compounds (like hydrogen sulfide) as electron donors.

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Purple non-sulfur bacteria

Purple bacteria that use organic molecules (like carbohydrates) as electron donors.

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Reverse electron flow

The process by which purple bacteria generate ATP after light absorption. It involves electrons flowing backwards through the electron transport chain.

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Calvin Cycle

The Calvin cycle is a series of metabolic reactions that fix CO2 to produce glucose. It's used by plants, cyanobacteria, and purple bacteria.

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CCCP

An example of a protonophore (uncoupler) that disrupts the proton gradient across the membrane, inhibiting ATP production.

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Reaction Center

A complex protein that captures light energy and drives the initial electron flow in photosynthesis.

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Great Oxidation Event

The Great Oxidation Event was a period in Earth's history when the atmosphere became oxygen-rich, primarily due to the emergence of oxygenic photosynthesis by cyanobacteria.

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Phycobilins

Phycobilins are accessory pigments found in cyanobacteria that absorb light energy and transfer it to chlorophyll.

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Prochlorococcus

Prochlorococcus is a type of cyanobacteria abundant in oceans, particularly in the mid-latitudes.

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Microbial mats

Microbial mats are layered communities of microorganisms, often including cyanobacteria, diatoms, and sulfur bacteria.

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Diatoms

Diatoms are a type of algae that use chlorophyll a for photosynthesis and are often found in microbial mats.

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Green sulfur bacteria

Green sulfur bacteria are photosynthetic bacteria that use sulfur compounds for energy and have unique chlorosomes for light harvesting.

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What are Green Sulfur Bacteria (GSB)?

Green sulfur bacteria (GSB) are anaerobic photosynthetic bacteria that use hydrogen sulfide (H2S) or other inorganic electron donors like thiosulfate or Fe2+ to generate energy. They deposit sulfur (S0) extracellularly.

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What are chlorosomes?

GSB have specialized light-harvesting structures called chlorosomes that allow them to grow in low-light environments, like deep waters or sediments.

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How do GSB fix carbon dioxide?

GSB use a modified version of the tricarboxylic acid (TCA) cycle, called the reverse TCA cycle, to fix carbon dioxide (CO2). This is different from the Calvin cycle used by plants and cyanobacteria.

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How are GSB's reaction centers like PSI?

GSB reaction centers resemble photosystem I (PSI) in cyanobacteria and chloroplasts, suggesting a common evolutionary origin.

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What is the electron donor crisis?

The electron donor crisis refers to the challenge faced by early photosynthetic organisms as they ran out of readily available electron donors like H2S. This led to the evolution of oxygenic photosynthesis.

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What is oxygenic photosynthesis?

Oxygenic photosynthesis uses water (H2O) as an electron donor, which is plentiful but difficult to oxidize. This process releases oxygen as a byproduct.

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How does oxygenic photosynthesis work?

In oxygenic photosynthesis, electrons flow through two photosystems (PSI and PSII) and produce ATP and NADPH. This allows for the fixation of CO2 via the Calvin cycle.

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Why was the development of oxygenic photosynthesis significant?

The development of oxygenic photosynthesis was a major evolutionary leap, as it allowed organisms to utilize the abundant water as an electron donor, leading to the rise of oxygen in Earth's atmosphere.

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Study Notes

Photosynthesis Lecture Notes

  • Diverse chlorophototroph bacteria are discussed.
  • Information is from Ann Rev Pl. Biol. 69:16 (2018).
  • S. Typhimurium utilizes waste products in its respiratory chain.
  • This bacterium outcompetes other bacteria due to its ability to use tetrathionate as a terminal electron acceptor, which it produces from thiosulfate found during inflammation.
  • Fermentative bacteria like lactic acid bacteria (LAB) produce ATP via substrate-level phosphorylation in glycolysis.
  • LAB still require a proton motive force (PMF) for transport, ion balance, and sometimes motility.
  • LAB use F₁F₀ ATPase in reverse as an ATP-driven proton pump.
  • ATPase/synthase is reversible, acting like a pump or a water wheel.
  • Bioenergetics involves electron transport chain moving electrons from donor to acceptor, outside the cell to the inside.
  • Glucose is converted to CO₂ releasing electrons, these are then used in the electron transport chain (oxidative phosphorylation) creating a large amount of ATP.
  • The next step after glucose and pyruvate and acetyl CoA is the conversion to CO₂ in the TCA cycle.
  • The main functions of photosynthesis include ATP generation via the electron transport chain which is light-driven and production of NAD(P)H for anabolic reactions, like CO₂ fixation, also light-driven.

Chlorophyll and Bacteriochlorophyll

  • Chlorophyll a and bacteriochlorophyll a are key molecules in photosynthesis for many anoxygenic phototrophs.
  • These molecules have absorption spectra in the visible and near-infrared region, with differences related to the organism's specific pigment.
  • These pigments are often associated with accessory pigments or proteins which broaden the wavelength absorption spectra.

Carotenoids

  • Carotenoids are accessory pigments that absorb light energy and increase the range of wavelengths that can be utilized by photosynthetic organisms.
  • Carotenoids are isoprenoids with conjugated double bonds and include beta-carotene, lycopene, and chlorobactene.

Photosynthetic Bacteria

  • There exist 7 phyla with photosynthetic members.
  • Oxygenic photosynthesis includes cyanobacteria.
  • Anoxygenic photosynthesis includes purple bacteria, green sulfur bacteria, green nonsulfur bacteria, heliobacteria, chloracidobacteria, and gemmatomonas.

More on Photosystems

  • Purple bacteria fix CO₂ using the reductive pentose phosphate (RPP), or Calvin cycle, same as plants and cyanobacteria.
  • The RPP cycle requires 18 ATP for each CO₂ fixed.
  • In all photosynthesis systems, the quinol/quinone pool has a key role in proton motive force ATP generation.
  • Light energy converts a weak electron donor into a strong electron donor in purple bacteria.
  • An example in bacterial mats are microbial communities, found with layers of photosynthetic bacteria.

Electron Donor Crisis

  • Anoxygenic photosynthetic bacteria use alternative electron donors available on early Earth, such as H₂S and Fe²⁺.
  • Oxygenic photosynthesis uses water (H₂O) as an electron donor, allowing for the conversion of CO₂ into sugars.

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