Phase Contrast Microscopy in Cell Study

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What effect does diffraction have on the brightness of the image in microscopy?

Causes flickering of brightness

Decreases the brightness (correct)

Increases the brightness

Has no impact on brightness

How does the thickness of the specimen affect the retardation of light in microscopy?

Decreases the speed of light (correct)

Reverses the direction of light

Increases the speed of light

No effect on the speed of light

In phase contrast microscopy, what happens when diffracted and undiffracted rays interfere?

Increase in brightness

No change in brightness

Change in color

Decrease in brightness (correct)

How does a phase contrast microscope enhance image contrast?

By reducing brightness Signup and view all the answers

What part of the positive phase plate do light rays from the specimen background pass through in a phase contrast microscope?

Thinner portion Signup and view all the answers

What is the primary function of a darkfield condenser in microscopy?

Enhance specimen contrast Signup and view all the answers

How does phase contrast microscopy enhance contrast in biological samples?

By increasing diffraction and scattering Signup and view all the answers

What is the main limitation of resolution in phase contrast microscopy?

The wavelength of visible light Signup and view all the answers

How does a darkfield condenser function differently from a phase plate in a microscope?

Darkfield condenser illuminates the specimen with oblique light while the phase plate accentuates phase changes Signup and view all the answers

Why is phase contrast microscopy particularly useful for studying living cells and tissues?

It allows observation of cell movement and cytoplasmic flow without staining Signup and view all the answers

What effect does a thicker part of a positive phase plate have on contrast in phase contrast microscopy?

Enhances contrast by increasing scattering Signup and view all the answers

How does special annulus and phase plates in a microscope contribute to phase contrast microscopy?

They amplify the phase changes produced by the specimen Signup and view all the answers

Why is it difficult to see cell contents using normal light microscopy?

Because living cells are colorless and transparent. Signup and view all the answers

What is the main advantage of phase contrast microscopy for examining live cells?

It enhances contrast in transparent and colorless live cells. Signup and view all the answers

How does darkfield microscopy differ from brightfield microscopy?

Darkfield microscopy uses a specialized darkfield condenser to enhance contrast. Signup and view all the answers

Why is staining not a suitable method for examining live cells?

Staining live cells can lead to disruption or cell death. Signup and view all the answers

What does phase contrast microscopy rely on to create contrast in images?

Differences in amplitude of light passing through the specimen. Signup and view all the answers

In which microscopy technique does the wavelength of light serve as a measure of color?

Phase-contrast microscopy Signup and view all the answers