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Questions and Answers
What effect does diffraction have on the brightness of the image in microscopy?
What effect does diffraction have on the brightness of the image in microscopy?
- Causes flickering of brightness
- Decreases the brightness (correct)
- Increases the brightness
- Has no impact on brightness
How does the thickness of the specimen affect the retardation of light in microscopy?
How does the thickness of the specimen affect the retardation of light in microscopy?
- Decreases the speed of light (correct)
- Reverses the direction of light
- Increases the speed of light
- No effect on the speed of light
In phase contrast microscopy, what happens when diffracted and undiffracted rays interfere?
In phase contrast microscopy, what happens when diffracted and undiffracted rays interfere?
- Increase in brightness
- No change in brightness
- Change in color
- Decrease in brightness (correct)
How does a phase contrast microscope enhance image contrast?
How does a phase contrast microscope enhance image contrast?
What part of the positive phase plate do light rays from the specimen background pass through in a phase contrast microscope?
What part of the positive phase plate do light rays from the specimen background pass through in a phase contrast microscope?
What is the primary function of a darkfield condenser in microscopy?
What is the primary function of a darkfield condenser in microscopy?
How does phase contrast microscopy enhance contrast in biological samples?
How does phase contrast microscopy enhance contrast in biological samples?
What is the main limitation of resolution in phase contrast microscopy?
What is the main limitation of resolution in phase contrast microscopy?
How does a darkfield condenser function differently from a phase plate in a microscope?
How does a darkfield condenser function differently from a phase plate in a microscope?
Why is phase contrast microscopy particularly useful for studying living cells and tissues?
Why is phase contrast microscopy particularly useful for studying living cells and tissues?
What effect does a thicker part of a positive phase plate have on contrast in phase contrast microscopy?
What effect does a thicker part of a positive phase plate have on contrast in phase contrast microscopy?
How does special annulus and phase plates in a microscope contribute to phase contrast microscopy?
How does special annulus and phase plates in a microscope contribute to phase contrast microscopy?
Why is it difficult to see cell contents using normal light microscopy?
Why is it difficult to see cell contents using normal light microscopy?
What is the main advantage of phase contrast microscopy for examining live cells?
What is the main advantage of phase contrast microscopy for examining live cells?
How does darkfield microscopy differ from brightfield microscopy?
How does darkfield microscopy differ from brightfield microscopy?
Why is staining not a suitable method for examining live cells?
Why is staining not a suitable method for examining live cells?
What does phase contrast microscopy rely on to create contrast in images?
What does phase contrast microscopy rely on to create contrast in images?
In which microscopy technique does the wavelength of light serve as a measure of color?
In which microscopy technique does the wavelength of light serve as a measure of color?
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