Phase-Contrast Microscopy
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Questions and Answers

What is the primary advantage of phase-contrast microscopy?

  • It produces images with a three-dimensional aspect.
  • It enables the study of living, unstained cells and tissues. (correct)
  • It allows for the examination of stained and fixed cells.
  • It enhances the optical density of cellular structures.

    • What principle is phase-contrast microscopy based on?

  • The refraction of light through cellular structures with different refractive indices. (correct)
  • The absorption of light by cellular pigments and dyes.
  • The polarization of light as it passes through the specimen.
  • The fluorescence emission of specific cellular components.

    • Which statement about phase-contrast microscopy is NOT true?

  • It requires the use of special staining techniques. (correct)
  • It produces images from transparent objects.
  • It is a prominent tool in cell culture laboratories.
  • It can be used to study unstained tissue sections.

    • What is the purpose of differential interference contrast microscopy with Nomarski optics?

    <p>To produce images with a more apparent three-dimensional aspect.</p> Signup and view all the answers

    Which microscopic technique is NOT mentioned in the text?

    <p>Fluorescence microscopy</p> Signup and view all the answers

    What is the main limitation of phase-contrast microscopy mentioned in the text?

    <p>The text does not mention any specific limitation.</p> Signup and view all the answers

    What type of specimens are typically studied using phase-contrast microscopy?

    <p>Unstained cells and tissues with similar optical densities</p> Signup and view all the answers

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