Pharmacology: MIC Analysis of Drug X
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Questions and Answers

What is the wavelength at which the absorbance values were obtained through spectrophotometric analysis?

  • 550 nm
  • 650 nm
  • 350 nm
  • 450 nm (correct)
  • Which of the following NSAIDs exhibited inhibitory activity against gram-negative E. coli at a MIC of 2.5 mg/mL?

  • Drug X
  • Ibuprofen and Paracetamol (correct)
  • Neither Ibuprofen nor Paracetamol
  • Paracetamol
  • What is the indication of a ZOI of 0 mm for both concentrations tested?

  • No effect on bacterial growth
  • Lack of antibacterial susceptibility (correct)
  • Partial antibacterial susceptibility
  • Complete antibacterial susceptibility
  • What is the significance of the MIC values of 2.5 mg/mL and 1.25 mg/mL in the context of the study?

    <p>They are the lowest concentrations tested</p> Signup and view all the answers

    What can be inferred from the results of the Kirby-Bauer test?

    <p>Drug X lacks antibacterial properties against E. coli and S. aureus</p> Signup and view all the answers

    What is the purpose of determining the Minimum Inhibitory Concentration (MIC) in the study?

    <p>To determine the effectiveness of NSAIDs against bacterial growth</p> Signup and view all the answers

    What is the primary purpose of determining the Minimum Inhibitory Concentration (MIC) of Drug X?

    <p>To evaluate the antibacterial susceptibility of E.coli and S.aureus</p> Signup and view all the answers

    What was the main advantage of using laboratory-grown bacterial strains in the experiment?

    <p>It safeguarded against acquiring personal information from human subjects and protected privacy</p> Signup and view all the answers

    What is the relationship between the Minimum Inhibitory Concentration (MIC) and the antibacterial susceptibility of E.coli and S.aureus?

    <p>A lower MIC indicates higher antibacterial susceptibility</p> Signup and view all the answers

    Which of the following NSAIDs was not used in the experiment to determine the Minimum Inhibitory Concentration (MIC)?

    <p>Aspirin</p> Signup and view all the answers

    What is the purpose of calculating the total sums of squares in the experiment?

    <p>To analyze the variance in the experiment</p> Signup and view all the answers

    What is the protocol used to determine the Minimum Inhibitory Concentration (MIC) of Drug X?

    <p>The protocol established by Baltzar (2017)</p> Signup and view all the answers

    What is the relationship between drug concentration and absorbance value in aureus?

    <p>Inversely proportional</p> Signup and view all the answers

    What is the effect of decreasing drug concentration on E. coli?

    <p>Decrease in absorbance value</p> Signup and view all the answers

    What do the MIC results of Drug X suggest about its activity against S. aureus and E. coli?

    <p>Potential inhibitory activity</p> Signup and view all the answers

    What is the effect of increasing Ibuprofen and Paracetamol concentration on S. aureus based on the ZOI results?

    <p>Increased susceptibility</p> Signup and view all the answers

    What was observed in S. aureus at a MIC of 1.25 mg/mL according to AL-Janabi (2010)?

    <p>Increased susceptibility</p> Signup and view all the answers

    What was observed in the study by Abd-El-Aziz et al. (2020) regarding Paracetamol?

    <p>Increased antibacterial activity</p> Signup and view all the answers

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