Pharmacology: MIC Analysis of Drug X

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What is the wavelength at which the absorbance values were obtained through spectrophotometric analysis?

450 nm

Which of the following NSAIDs exhibited inhibitory activity against gram-negative E. coli at a MIC of 2.5 mg/mL?

Ibuprofen and Paracetamol

What is the indication of a ZOI of 0 mm for both concentrations tested?

Lack of antibacterial susceptibility

What is the significance of the MIC values of 2.5 mg/mL and 1.25 mg/mL in the context of the study?

<p>They are the lowest concentrations tested</p> Signup and view all the answers

What can be inferred from the results of the Kirby-Bauer test?

<p>Drug X lacks antibacterial properties against E. coli and S. aureus</p> Signup and view all the answers

What is the purpose of determining the Minimum Inhibitory Concentration (MIC) in the study?

<p>To determine the effectiveness of NSAIDs against bacterial growth</p> Signup and view all the answers

What is the primary purpose of determining the Minimum Inhibitory Concentration (MIC) of Drug X?

<p>To evaluate the antibacterial susceptibility of E.coli and S.aureus</p> Signup and view all the answers

What was the main advantage of using laboratory-grown bacterial strains in the experiment?

<p>It safeguarded against acquiring personal information from human subjects and protected privacy</p> Signup and view all the answers

What is the relationship between the Minimum Inhibitory Concentration (MIC) and the antibacterial susceptibility of E.coli and S.aureus?

<p>A lower MIC indicates higher antibacterial susceptibility</p> Signup and view all the answers

Which of the following NSAIDs was not used in the experiment to determine the Minimum Inhibitory Concentration (MIC)?

<p>Aspirin</p> Signup and view all the answers

What is the purpose of calculating the total sums of squares in the experiment?

<p>To analyze the variance in the experiment</p> Signup and view all the answers

What is the protocol used to determine the Minimum Inhibitory Concentration (MIC) of Drug X?

<p>The protocol established by Baltzar (2017)</p> Signup and view all the answers

What is the relationship between drug concentration and absorbance value in aureus?

<p>Inversely proportional</p> Signup and view all the answers

What is the effect of decreasing drug concentration on E. coli?

<p>Decrease in absorbance value</p> Signup and view all the answers

What do the MIC results of Drug X suggest about its activity against S. aureus and E. coli?

<p>Potential inhibitory activity</p> Signup and view all the answers

What is the effect of increasing Ibuprofen and Paracetamol concentration on S. aureus based on the ZOI results?

<p>Increased susceptibility</p> Signup and view all the answers

What was observed in S. aureus at a MIC of 1.25 mg/mL according to AL-Janabi (2010)?

<p>Increased susceptibility</p> Signup and view all the answers

What was observed in the study by Abd-El-Aziz et al. (2020) regarding Paracetamol?

<p>Increased antibacterial activity</p> Signup and view all the answers

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